Live-action films of worm sperm help researchers track critical fertility enzymes

November 1, 2011
This is a micrograph of a C. elegans ameoboid sperm. Credit: Aiza Go and Diana Chu

Compared to most other cells in an organism, sperm undergo a radical transformation to become compact and mobile delivery systems for paternal DNA. Even though sperm looks and moves quite differently across species, SF State researcher Diana Chu and colleagues now say that there are at least a few key enzymes that are critical for sperm development and mobility in species as different as mice and nematode worms.

The study by Chu, et al., was published today online by the journal Genetics.

These enzymes (called PP1 phosphatases) are multitaskers in the nematode, which Chu and the others discovered through unique live-action films of the enzymes at work. First, the enzymes help to separate during sperm cell division. After that, they play a role in the development of the sperm's pseudopods—the appendages that nematode sperm use to move. Pseudopods propel the sperm with a "treadmilling" motion, and the enzymes help disassemble the cell's inner skeleton in a way that pushes the treadmilling forward.

Fluorescent images of C. elegans sperm. Shown in red are GSP-3/4, phosphatases required for fertility, white are membranous organelles and green is major sperm protein, a C. elegans protein important for motility. GSP-3/4 is shown to be an important regulator of MSP in the Wu et al. paper. Credit: Jui-ching Wu and Diana Chu

Sperm in mammals like mice—and men—don't have pseudopods and don't move in the same way, but they still rely on the phosphatases for development and mobility. Further study of the phosphatases could someday shed light on some of the causes of human infertility, since the enzymes seem to be critical for sperm function.

More information: "PP1 phosphatases regulate multiple stages of sperm development and motility in Caenorhabditis elegans," published in advance online by Genetics. www.genetics.org/content/early/recent

Related Stories

Recommended for you

How our cells use mother's and father's genes

September 28, 2016

Researchers at Karolinska Institutet and Ludwig Institute for Cancer Research have characterized how and to what degree our cells utilize the gene copies inherited from our mother and father differently. At a basic level ...

Questions, concerns about 'three-parent' baby

September 28, 2016

The surprise announcement that a healthy baby boy was born from a new technique mixing the DNA of two women and a man raises as many questions—scientific and ethical—as it settles, experts said Wednesday.

Regulatory RNA essential to DNA damage response

September 26, 2016

Stanford researchers have found that a tumor suppressor known as p53 is stabilized by a regulatory RNA molecule called DINO. The interaction helps a cell respond to DNA damage and may play a role in cancer development and ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.