A new application allows online statistical analysis of gene-expression data

The journal Computers in Biology and Medicine has published an article on the new IT application BootstRatio, created by IDIBELL researchers. The application allows online statistical analysis of data from gene expression. It is accessible through http://regstattools.net/br and any scientist is already to use it.

Researchers at the group at IDIBELL, led by Dr. Virginia Nunes, had a problem to provide signification to the results of of gene expression data. Most of the statistical calculations which are done to compare assume a normal distribution of data and this distribution is not always real.

Together with Ramon Clèries, statistician at IDIBELL's Cancer Prevention and Control group, Dr. Nunes decided to work on a using resampling techniques that allows to generate a hypothetical distribution of the data without any initial distribution and exposing the data in a comprehensible language to the scientist.

That was the beginning of BootstRatio, which can be accessed from a remote server. "After the researcher enters the data, in a few time the program sends the results via email in a comprehensible language to the scientist," explains Miguel Lopez de Heredia, CIBERER researcher at the Human Molecular Genetics group at IDIBELL. "Statisticians and biologists sometimes speak different languages. This new tool allows a biological interpretation of the results of statistical analysis. "

Dr. Clèries explains that the application BootstRatio "is very easy to use and it doesn't require statistical expertise."

More information: Clèries R., Galvez J., Espino M., Ribes J., Nunes V. and López de Heredia M. BootsRatio: A web-based stsatistical analysis of fold-change in qPCR and RT-qPCR data using resampling methods. Computers in Biology and Medicine 42 (2012) 438-445. doi: 10.1016/j.compbiomed.2011.12-012

Provided by IDIBELL-Bellvitge Biomedical Research Institute

not rated yet
add to favorites email to friend print save as pdf

Related Stories

BRG1 mutations confer resistance to hormones in lung cancer

Mar 15, 2012

Retinoic acid (vitamin A) and steroids are hormones found in our body that protect against oxidative stress, reduce inflammation and are involved in cellular differentiation processes. One of the characteristics of tumours ...

Cloud computing method greatly increases gene analysis

Sep 08, 2010

Researchers at the Johns Hopkins Bloomberg School of Public Health have developed new software that greatly improves the speed at which scientists can analyze RNA sequencing data. RNA sequencing is used to compare differences ...

Recommended for you

Mysterious esophagus disease is autoimmune after all

5 hours ago

(Medical Xpress)—Achalasia is a rare disease – it affects 1 in 100,000 people – characterized by a loss of nerve cells in the esophageal wall. While its cause remains unknown, a new study by a team of researchers at ...

Diagnostic criteria for Christianson Syndrome

Jul 21, 2014

Because the severe autism-like condition Christianson Syndrome was only first reported in 1999 and some symptoms take more than a decade to appear, families and doctors urgently need fundamental information ...

New technique maps life's effects on our DNA

Jul 20, 2014

Researchers at the BBSRC-funded Babraham Institute, in collaboration with the Wellcome Trust Sanger Institute Single Cell Genomics Centre, have developed a powerful new single-cell technique to help investigate how the environment ...

User comments