Human antibody for dengue virus isolated
A TEM micrograph showing Dengue virus virions (the cluster of dark dots near the center). Image: CDC
(Phys.org) -- A group of scientists in Singapore and the UK have isolated a human antibody capable of effectively neutralizing the mosquito-borne dengue virus. Dengue fever is currently incurable and infects an estimated 100,000 people a year, mostly in the tropics. The only treatment is alleviating the symptoms, which can include intense joint and muscle pain, nausea, vomiting, high fever, and death in severe cases.
Dengue virus (DENV) has four strains or serotypes (1 to 4), and a person infected by one serotype produces antibodies that make them immune for life to infection from that serotype, but that usually only give limited or transient immunity to the other three. The newly isolated antibody is extremely effective for serotype 1.
The researchers isolated the human antibody, HM14c10, which was formed in the body of a patient in Singapore who had recovered from a DENV1 infection. The antibody turned out to be extremely fast-acting and gave powerful immunity to the virus.
The group recruited around 100 recovered dengue patients and found over 200,000 antibodies in total. The HM14c10 antibody turned out to be so powerful that it kills the virus before it is able to infect the cells, according to lead researcher, Professor Lok of the National University of Singapore.
After isolating the antibody the researchers carried out experiments on mice and discovered that it functions by stretching across the virus surface, preventing the changes to its surface proteins that must take place for the virus to be able to infect cells.
The paper was published in the journal Science Translational Medicine, and the findings may help researchers develop new therapies to treat or prevent infection by the dengue virus. The research showed the antibody to be far more effective at neutralizing viruses than the anti-dengue chemicals now in development.
The next phase of the research on DENV1 will be clinical trials to test the antibody on patients infected with DENV1. The team will also continue to check the remaining antibodies in their library to determine if any are as effective against the other serotypes, and they have already found a likely candidate against serotype 2.
Another lead author of the paper, Dr Paul A. MacAry of the National University of Singapore said that in Singapore around 90% of dengue fever cases were either DENV1 or 2, and their research should lead to an antibody for each of these strains within about six months.
More information: The Structural Basis for Serotype-Specific Neutralization of Dengue Virus by a Human Antibody, Sci Transl Med 20 June 2012:
Vol. 4, Issue 139, p. 139ra83. DOI: 10.1126/scitranslmed.3003888
Dengue virus (DENV) is a mosquito-borne flavivirus that affects 2.5 billion people worldwide. There are four dengue serotypes (DENV1 to DENV4), and infection with one elicits lifelong immunity to that serotype but offers only transient protection against the other serotypes. Identification of the protective determinants of the human antibody response to DENV is a vital requirement for the design and evaluation of future preventative therapies and treatments. Here, we describe the isolation of a neutralizing antibody from a DENV1-infected patient. The human antibody 14c10 (HM14c10) binds specifically to DENV1. HM14c10 neutralizes the virus principally by blocking virus attachment; at higher concentrations, a post-attachment step can also be inhibited. In vivo studies show that the HM14c10 antibody has antiviral activity at picomolar concentrations. A 7 Å resolution cryoelectron microscopy map of Fab fragments of HM14c10 in a complex with DENV1 shows targeting of a discontinuous epitope that spans the adjacent surface of envelope protein dimers. As found previously, a human antibody specific for the related West Nile virus binds to a similar quaternary structure, suggesting that this could be an immunodominant epitope. These findings provide a structural and molecular context for durable, serotype-specific immunity to DENV infection.
Journal reference: Science Translational Medicine
© 2012 Phys.Org
- Rethinking the antibody-dependent enhancement dengue hemorrhagic fever model Oct 26, 2009 | not rated yet | 0
- Analyzing disease transmission at the community level May 28, 2012 | not rated yet | 0
- New understanding of dengue fever could help with vaccine May 06, 2010 | not rated yet | 0
- Scientists prove hypothesis on the mystery of dengue virus infection Feb 11, 2010 | not rated yet | 0
- Human clinical trial of NIH-developed dengue vaccine begins Aug 09, 2010 | not rated yet | 0
- Motion perception revisited: High Phi effect challenges established motion perception assumptions Apr 23, 2013 | 3 / 5 (2) | 2
- Anything you can do I can do better: Neuromolecular foundations of the superiority illusion (Update) Apr 02, 2013 | 4.5 / 5 (11) | 5
- The visual system as economist: Neural resource allocation in visual adaptation Mar 30, 2013 | 5 / 5 (2) | 9
- Separate lives: Neuronal and organismal lifespans decoupled Mar 27, 2013 | 4.9 / 5 (8) | 0
- Sizing things up: The evolutionary neurobiology of scale invariance Feb 28, 2013 | 4.8 / 5 (10) | 14
Classical and Quantum Mechanics via Lie algebras
Apr 15, 2011 I'd like to open a discussion thread for version 2 of the draft of my book ''Classical and Quantum Mechanics via Lie algebras'', available online at http://lanl.arxiv.org/abs/0810.1019 , and for the...
- More from Physics Forums - Independent Research
More news stories
Saudi Arabia said Friday it would send samples taken from animals possibly infected with a deadly SARS-like virus to the United States for testing in a bid to find the source of disease.
Diseases, Conditions, Syndromes 1 hour ago | not rated yet | 0
The World Health Organization voiced deep concern Thursday over the SARS-like virus that has killed 22 people in less than a year, saying it might potentially spread more widely between humans.
Diseases, Conditions, Syndromes 5 hours ago | 5 / 5 (1) | 0
International efforts to combat a new pneumonia-like virus that has now killed 22 people are being slowed by unclear rules and competition for the potentially profitable rights to disease samples, the head ...
Diseases, Conditions, Syndromes 17 hours ago | not rated yet | 0
(HealthDay)—A shortage of a critical tuberculosis drug has hampered the efforts of health departments across the United States to contain the spread of the highly infectious lung disease, federal officials ...
Diseases, Conditions, Syndromes 18 hours ago | not rated yet | 0
Maintaining a heart healthy lifestyle may also help protect chronic kidney disease patients from developing kidney failure and dying prematurely, according to a study appearing in an upcoming issue of the Journal of the Am ...
Diseases, Conditions, Syndromes 18 hours ago | not rated yet | 0
Biological processes are generally based on events at the molecular and cellular level. To understand what happens in the course of infections, diseases or normal bodily functions, scientists would need to ...
10 minutes ago | not rated yet | 0 |
The news about youth and diabetes keeps getting worse. The latest data from the national TODAY diabetes study shows that children who develop Type 2 diabetes are at high risk to develop heart, kidney and eye problems faster ...
8 minutes ago | not rated yet | 0
In Parkinson's disease, the protein "alpha-synuclein" aggregates and accumulates within neurons. Specific areas of the brain become progressively affected as the disease develops and advances. The mechanism underlying this ...
7 minutes ago | not rated yet | 0
Transcatheter aortic valve implantation (TAVI) is effective and safe in Asian patients, according to early experience based on first results from a multicentre Asian registry reported at EuroPCR 2013.
5 minutes ago | not rated yet | 0
Young children who missed more than half of recommended well-child visits had up to twice the risk of hospitalization compared to children who attended most of their visits, according to a study published today in the American Jo ...
9 minutes ago | not rated yet | 0
(Medical Xpress)—A new study by researchers in the US has shown that an ancient virus can be modified to help in the fight against the simian immunodeficiency virus SIV, which is the equivalent in monkeys ...
2 hours ago | 5 / 5 (3) | 0 |