The language of neural cells

by Angela Herring
Heather Clark uses nanosensors to monitor the chemical environment of various biological systems.

Imagine if we could under­stand the lan­guage two neu­rons use to com­mu­ni­cate. We might learn some­thing about how thoughts and con­scious­ness are formed. At the very least, our improved under­standing of neuron com­mu­ni­ca­tion would help biol­o­gists study the brain with more pre­ci­sion than ever before.

Heather Clark, an asso­ciate pro­fessor of phar­ma­ceu­tical sci­ences at North­eastern Uni­ver­sity, has received a $300,000 Young Fac­ulty Award from the to explore neural cell com­mu­ni­ca­tion using her exper­tise in .

"We were inter­ested in looking into because of the need to mea­sure chem­i­cals in the brain," she explained.

In sep­a­rate work, Clark had already been devel­oping nanosen­sors to mea­sure the bio­chem­ical envi­ron­ment inside a single neuron. The DARPA award will allow her team to extend the inves­ti­ga­tion to the bio­chem­ical reac­tions between cells. "We will use the chem­istry devel­oped for another DARPA project and apply it to making a sensor for neu­ro­trans­mitter release," Clark said.

The other DARPA project, she noted, enabled the team to incor­po­rate enzymes into their sensor format. Post­doc­toral researcher Kevin Cash did the bulk of this work, devel­oping sen­sors that mon­itor the byprod­ucts of enzy­matic reactions.

As Clark put it, "We can mea­sure a whole lot more mol­e­cules that way."

When a neural cell sends a mes­sage to another cell, it releases mol­e­cules into the inter­cel­lular envi­ron­ment. The new nanosensor will incor­po­rate an enzyme that reacts with these mol­e­cules to pro­duce acidity changes. A pH mon­itor also incor­po­rated into the nanosensor will rec­og­nize those acidity changes and pro­duce a signal equiv­a­lent to the con­cen­tra­tion of the neurotransmitter.

This indi­rect mea­sure­ment process will allow Clark's team to mea­sure neu­ro­trans­mitter levels without directly binding to them, a chal­lenge in its own right. "But enzymes do a really great job of rec­og­nizing [these mol­e­cules] nat­u­rally," said Clark. "It's what they do best."

The researchers have devel­oped other sen­sors that use this same chem­ical pro­tocol, but the dif­fi­culty lies in get­ting them into the inter­cel­lular space. "We're not actu­ally working in the whole brain, we're working in slices [of the mouse brain]," Clark explained. "So we're hoping to just flush it into the tissue layer."

Col­lab­o­ra­tors at Har­vard Med­ical School will apply their exper­tise in keeping brain tissue alive in solu­tion for these types of tests. But before reaching that level of inves­ti­ga­tion, the North­eastern researchers will val­i­date the nanosen­sors in in vitro and model cel­lular settings.

In addi­tion to receiving funding, YFA awardees are paired with DARPA pro­gram man­agers who share sim­ilar inter­ests. Clark's mentor is a med­ical doctor with exper­tise in microscale and nanoscale bio­med­ical tech­nolo­gies. "It's always my goal to do some­thing that is med­ically and clin­i­cally rel­e­vant," Clark said. "I think that will be good insight."

Related Stories

Fat for better drug function

date Aug 09, 2012

Sci­en­tists have long known that food diges­tion affects the way the body absorbs var­ious compounds—from nutri­ents to drugs and toxins.

Chipping away at cancer

date Jun 25, 2012

(Medical Xpress) -- In the last two decades, the number of deaths from col­orectal cancer has steadily declined, according to the Amer­ican Cancer Society. While some of the decrease can be attrib­uted ...

Recommended for you

Study on neurogenesis in the olfactory bulb

date May 21, 2015

A new study published by the team of Naguib Mechawar, Ph.D., a researcher at the Douglas Institute (CIUSSS de l'Ouest-de-l'île-de-Montréal) and Associate Professor in the Department of Psychiatry at McGill University, suggests ...

User comments

Adjust slider to filter visible comments by rank

Display comments: newest first

Tausch
Aug 23, 2012
Control climate and you control the world.
Control the molecular ('langauge') and you control the mind.

"It's always my goal to do some­thing that is med­ically and clin­i­cally rel­e­vant," Clark said. "I think that will be good insight."


Yes. Only in light of the esuing ethnics.

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.