Novel technique demonstrates interactions between malaria parasite and HIV
Here, scientist Guadalupe Andreani prepares cells for culture. Credit: Journal of Visualized Experiments
The World Health Organization estimates that in 2011 there were 216 million cases of malaria and 34.2 million people living with HIV. These diseases particularly afflict sub-Saharan Africa, where large incidence of co-infection result in high mortality rates. Yet, in spite of this global pandemic, interactions between the parasite that causes malaria, Plasmodium falciparum, and HIV-1 are poorly understood. However, a new video article in JoVE, the Journal of Visualized Experiments, that describes a novel technique to study the interactions between HIV-1 and P. falciparum in cultured human cells, will allow scientists to explore different parameters of co-infection by the two microbes.
The study is led by Dr. David Richard of the Centre Hospitalier Universitaire de Quebec (CHUQ). Dr. Richard explains, "We don't know much about what is happening at the cellular level when HIV-1-infected immune cells encounter the malaria parasite. Results obtained from the few studies exploring the interaction of these two diseases are sometimes conflicting. We hope that our model will allow us to thoroughly dissect these interactions in a simplified system."
Each disease attacks a different component of human blood, thus disturbing normal immune function. P. falciparum infect red blood cells and cause fever, shivering, vomiting, or convulsions in patients. HIV-1 causes acquired immune deficiency syndrome (AIDS) by infecting components of the immune system, including macrophages and helper T cells, and then replicates and destroys the host cells. By studying co-infection at different phases of each disease in vitro, scientists can better understand how different stages of malaria infection and HIV reproduction affect the onset and severity of the other disease. As such, Dr. Richard and his laboratory present a technique that investigates how P. falciparum-infected red blood cells affect the replication of HIV-1 in monocyte-derived macrophages.
Dr. Richard points out that, "by publishing in JoVE, you really see what is happening in the experiment. The visual representation helps succinctly explain a long procedure, and gives you a fuller picture of the schematic complexity." He hopes that this publication will give the scientific community the tools to look at the interactions on a cellular level, which would be an initial step in improving the quality of life for individuals infected by these deadly diseases. "This protocol provides a tool to examine the interactions between P. falciparium and HIV," states JoVE editor Dr. Charlotte Frank Sage, "Publication of the protocol in JoVE will allow researchers around the world to see a detailed demonstration of this system which will help in bring the technology to their laboratories."
More information: Richard et. al.: www.jove.com/video… iparum-hiv-1
Journal reference: Journal of Visualized Experiments
Provided by The Journal of Visualized Experiments
- Exhausted B cells fail to fight HIV Jul 14, 2008 | not rated yet | 0
- Improving human immunity to malaria Aug 01, 2012 | not rated yet | 0
- Scientists find another key to HIV success Mar 22, 2006 | not rated yet | 0
- Researchers discuss challenges to developing broadly protective HIV vaccines Sep 07, 2011 | not rated yet | 0
- Unveiling malaria's 'invisibility cloak' Jan 18, 2012 | not rated yet | 0
- Motion perception revisited: High Phi effect challenges established motion perception assumptions Apr 23, 2013 | 3 / 5 (2) | 2
- Anything you can do I can do better: Neuromolecular foundations of the superiority illusion (Update) Apr 02, 2013 | 4.5 / 5 (11) | 5
- The visual system as economist: Neural resource allocation in visual adaptation Mar 30, 2013 | 5 / 5 (2) | 9
- Separate lives: Neuronal and organismal lifespans decoupled Mar 27, 2013 | 4.9 / 5 (8) | 0
- Sizing things up: The evolutionary neurobiology of scale invariance Feb 28, 2013 | 4.8 / 5 (10) | 14
Classical and Quantum Mechanics via Lie algebras
Apr 15, 2011 I'd like to open a discussion thread for version 2 of the draft of my book ''Classical and Quantum Mechanics via Lie algebras'', available online at http://lanl.arxiv.org/abs/0810.1019 , and for the...
- More from Physics Forums - Independent Research
More news stories
(AP)—Alabama health officials say a mysterious respiratory illness has left five people hospitalized and two dead in the southeastern part of the state.
Diseases, Conditions, Syndromes 11 minutes ago | not rated yet | 0
A paper recently published in the New England Journal of Medicine and co-written by physicians and scientists at the University of Colorado School of Medicine finds that an important genetic risk factor for pulmonary fibros ...
Diseases, Conditions, Syndromes 13 hours ago | not rated yet | 0
Using the Department of Defense Serum Repository (DoDSR), University of Cincinnati (UC) researchers have identified a number of biomarkers for inflammatory bowel disease (IBD), which could help with earlier diagnosis and ...
Diseases, Conditions, Syndromes 13 hours ago | 5 / 5 (1) | 0
(HealthDay)—Three-quarters of public schools in the metro Atlanta area contain microbes, including bacteria indicating the presence of fecal matter, according to research published in the May 17 issue of ...
Diseases, Conditions, Syndromes 14 hours ago | not rated yet | 0
(HealthDay)—Factors such as increased case finding may explain why Michigan had half of the total spinal infections associated with contaminated methylprednisolone acetate in the recent fungal meningitis ...
Diseases, Conditions, Syndromes 15 hours ago | not rated yet | 0
Italian lawmakers on Wednesday gave their final approval to a law that allows limited use of a controversial type of stem cell therapy which has been condemned by many scientists but has given hope to families of terminally-ill ...
1 minute ago | not rated yet | 0
(AP)—The mayor of Portland, Ore., has conceded defeat in an effort to add fluoride to the city's drinking water.
30 minutes ago | not rated yet | 0
Is it permissible to harm one to save many? Those who tend to say "yes" when faced with this classic dilemma are likely to be deficient in a specific kind of empathy, according to a report published in the scientific journal ...
2 hours ago | not rated yet | 0 |
Phthalates: Study links chemicals widely found in plastics, processed food to elevated blood pressure in children, teens
Plastic additives known as phthalates (pronounced THAL-ates) are odorless, colorless and just about everywhere: They turn up in flooring, plastic cups, beach balls, plastic wrap, intravenous tubing and—according to the ...
3 hours ago | not rated yet | 1 |
(Medical Xpress)—Native peoples in regions where cameras are uncommon sometimes react with caution when their picture is taken. The fear that something must have been stolen from them to create the photo ...
19 hours ago | 4.2 / 5 (5) | 0 |