Congenital disease linked to adipocyte development

Congenital disease linked to adipocyte development
Some patients with congenital generalized lipodystrophy, who lack adipocytes and develop severe insulin resistance, have a defect in adipocyte development that can be partially reversed, according to a study published online Aug. 28 in Diabetes.

(HealthDay)—Some patients with congenital generalized lipodystrophy (CGL), who lack adipocytes and develop severe insulin resistance, have a defect in adipocyte development that can be partially reversed, according to a study published online Aug. 28 in Diabetes.

To identify the defect in adipogenesis in patients with CGL due to mutations in AGPAT2, Angela R. Subauste, M.D., from the University of Michigan in Ann Arbor, and colleagues studied adipogenesis using 3T3-L1 preadipocytes with knockdown or of AGPAT2, as well as muscle-derived multipotent cells from controls and from patients harboring AGPAT2 mutations.

The researchers found that individuals with mutated AGPAT2 had an adipogenic defect which was rescued by a retrovirus expressing AGPAT2. Inducing adipogenesis led to increased cell death in the absence of AGPAT2. Akt activation was reduced by lack of AGPAT2, and lipogenesis was partially restored by overexpression of constitutively active AKT. AGPAT2 modulated the lipome, altering activation of the phosphatidylinositol 3-kinase/Akt and peroxisome proliferator-activated receptor gamma (PPARγ) pathways. The adipogenic defect in CGL cells was partially rescued by the PPARγ agonist pioglitazone.

"Our findings demonstrate that CGL secondary to AGPAT2 mutation is not a disease of empty adipocytes, rather a disease of defective adipose due to a disruption in the modulation of the lipome during adipogenesis," Subauste and colleagues conclude.

More information: Abstract
Full Text (subscription or payment may be required)

add to favorites email to friend print save as pdf

Related Stories

Fat-free diet reduces liver fat in fat-free mice

Feb 03, 2009

Researchers at UT Southwestern Medical Center have uncovered crucial clues about a paradoxical disease in which patients with no body fat develop many of the health complications usually found in obese people.

Researchers find more clues to causes of breast cancer

Oct 27, 2011

Publishing in the current issue of The Journal of Biological Chemistry (Vol. 286, No 43), researchers at Moffitt Cancer Center in Tampa, Fla., have discovered additional mechanisms of "Akt" activation and suggest a component ...

Recommended for you

Scientist finds clearer obesity, diabetes connection

Aug 27, 2014

(Medical Xpress)—New findings about the biological links between obesity and insulin resistance and Type 2 diabetes may also shed light on the connection between obesity and cancer, says a scientist at ...

User comments