Improved outlook for immune-based therapies: Assay identifies T cells most capable of fighting infections and cancers

Prof. Dirk Busch is director of the Institute for Medical Microbiology, Immunology, and Hygiene at the Technische Universitaet Muenchen (TUM) and host of the TUM Institute for Advanced Study focus group on clinical cell processing and purification. Technology for the T-cell "off rate" assay, which identifies T cells most likely to be effective in adoptive transfer therapy, was developed primarily in his lab and tested in collaboration with partners in Seattle, Mainz, and Berlin. Credit: Astrid Eckert, copyright TUM

The idea of fighting infections and even cancers by inducing protective immune responses may now be a step closer to clinical practice. Researchers have removed a major obstacle to widespread use of so-called adoptive transfer therapy, in which a patient receives "killer" immune cells targeting a disease agent. Existing technologies can easily provide T cells that will recognize a specific antigen, but it has been challenging to identify individual cells most likely to succeed in fighting the disease – until now.

Scientists at the Technische Universitaet Muenchen (TUM) have developed the first assay for living T that can quantify how strongly the cells' bind to their targeted antigen. Optimal binding strength or "structural avidity" is thought to be the most important predictor of success in adoptive transfer therapies. Together with collaborators in Seattle, Mainz, and Berlin, the TUM researchers have successfully tested their assay in preclinical animal models very close to and with human T cells. They published their results in the journal Science Translational Medicine.

A new key to predictability

There are countless kinds of T cells, each one expressing a single type of receptor—a capable of locking onto a particular ligand molecule that may, for example, be associated with a virus or a tumor. If a patient's immune system cannot mount an effective defense, it may be possible to fight the disease by introducing the right kind of T cell, either prepared from the patient's own cells or obtained from a donor.

But in a population of T cells equipped to recognize a particular antigen, individual T cells can vary greatly in structural avidity. A main determinant of a T cell's binding strength is the so-called "off rate"—simply put, a slower off rate indicates stronger binding—which thus predicts its likely fitness for use in adoptive transfer therapy. And that is what the TUM assay measures, directly and precisely.

The TUM technology offers three marked advantages over previous methods: It is (relatively) simple; it can be used on the surface of living T cells without harming or changing them; and it gives, for the first time, a quantitative measure of . "This is a true value that we can repeat, and we can compare individual cells on the basis of these measurements," explains Prof. Dirk Busch, director of the TUM Institute for Medical Microbiology, Immunology, and Hygiene. "Furthermore, we now have been able to show that there is indeed a correlation between these values and what the cells can do when we put them into a clinically relevant setting."

Bridging the distance from the labs to the clinic

The Munich-based team had been working on this technology and the underlying scientific questions for nearly a decade before making one crucial connection—to Seattle. With support from the TUM Institute for Advanced Study, a bridge was formed between Busch's labs and those of Prof. Stanley Riddell at the Fred Hutchinson Cancer Research Center, University of Washington.

A pioneer in cell-based therapy, Riddell had been looking for new ways to achieve higher quality and purity in cells for clinical use. Busch was seeking a partner with top-level expertise in human cell-therapy research, trials, and treatment. Together, they developed a close-knit collaboration across nine time zones that transformed both laboratories. Over the course of three years, Riddell spent months in Munich and hosted a number of Busch's postdocs and doctoral candidates, including some of the authors of the current paper, in Seattle. Weekly video conferences filled in the gaps.

As Busch describes it, though, the most significant gap they bridged was the distance from the labs to the clinic. "Stan taught us a lot about how to work with human T cells," he says, "and a lot of the data in this paper, in particular the data on human T cells, is a direct result of our TUM-IAS focus group on clinical cell processing and purification." He is optimistic about the prospects for the T-cell selection assay to play a role in clinical trials and ultimately in therapies, as well as in helping to address critical open questions about immune responses to major diseases.

"Immunotherapy is now beginning to have a major impact for patients with cancer and infectious diseases," Riddell says. "This work provides a novel approach for evaluating the qualities of T cells that may be used for cellular immunotherapy, and it facilitates the selection of those T cells that would be most effective in treating disease."

More information: TCR-ligand k-off rate predicts protective capacity of antigen-specific CD8+ T cells for adoptive transfer. Magdalena Nauerth, Bianca Weissbrich, Robert Knall, Tobias Franz, Georg Doessinger, Jeanette Bet, Paulina J. Paszkiewicz, Lukas Pfeifer, Mario Bunse, Wolfgang Uckert, Rafaela Holtappels, Dorothea Gillert-Marien, Michael Neuenhahn, Angela Krackhardt, Matthias J. Reddehase, Stanley R. Riddell, and Dirk H. Busch. Science Translational Medicine 2013, vol. 5, issue 192. DOI: 10.1126/scitranslmed.3005958

Related Stories

Universal donor immune cells

Jul 25, 2011

One of the latest attempts to boost the body's defenses against cancer is called adoptive cell transfer, in which patients receive a therapeutic injection of their own immune cells. This therapy, currently tested in early ...

Modified immune cells seek and destroy melanoma

Jun 24, 2013

In this issue of the Journal of Clinical Investigation, researchers led by Scott Pruitt at Duke University and Merck Research Laboratories report on a human clinical trial in which modified dendritic cells, a component of the ...

Recommended for you

Could trophoblasts be the immune cells of pregnancy?

Dec 18, 2014

Trophoblasts, cells that form an outer layer around a fertilized egg and develop into the major part of the placenta, have now been shown to respond to inflammatory danger signals, researchers from Norwegian University of ...

Moms of food-allergic kids need dietician's support

Dec 18, 2014

Discovering your child has a severe food allergy can be a terrible shock. Even more stressful can be determining what foods your child can and cannot eat, and constructing a new diet which might eliminate entire categories ...

Multiple allergic reactions traced to single protein

Dec 17, 2014

Johns Hopkins and University of Alberta researchers have identified a single protein as the root of painful and dangerous allergic reactions to a range of medications and other substances. If a new drug can ...

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.