Researchers learn how to break a sweat

by Marie Rippen
This image shows staining of slow-cycling sweat gland cells (green) with the protein laminin (red) and the fluorescent stain DAPI (blue). Credit: Yvonne Leung

Without sweat, we would overheat and die. In a recent paper in the journal PLOS ONE, USC faculty member Krzysztof Kobielak and a team of researchers explored the ultimate origin of this sticky, stinky but vital substance—sweat gland stem cells.

Kobielak and his team used a system to make all of the sweat gland cells in a mouse easy to spot: labeling them with (GFP), which is visible under ultraviolet light.

Over time, the GFP became dimmer as it was diluted among dividing sweat gland cells. After four weeks, the only cells that remained fluorescent were the ones that did not divide or divided very slowly—a known property among stem cells of certain tissues, including the and cornea. Therefore, these slow-dividing, fluorescent cells in the sweat gland's coiled lower region were likely also stem cells.

Then, the first author of this paper, graduate student Yvonne Leung, tested whether these fluorescent cells could do what stem cells do best—differentiate into multiple cell types. To the researchers' surprise, these glowing cells generated not only , but also hair follicles when placed in the skin of a mouse without GFP.

The researchers also determined that under certain conditions, the sweat gland could heal skin wounds and regenerate all layers of the epidermis.

"That was a big surprise for us that those very quiescent sweat gland stem maintain multilineage plasticity—participating not only in their own regeneration, but also in the regeneration of hair follicles and skin after injury," said Kobielak, assistant professor of pathology at the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC.

This offers exciting possibilities for developing future stem cell-based treatments for skin and sweat gland-related conditions, such as hyperhidrosis or hypohidrosis (excessive or insufficient sweating). It could also lay the foundation for creating fully functional skin—containing both glands and hair follicles—for burn victims.

More information: www.ncbi.nlm.nih.gov/pmc/articles/PMC3776797/

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