Modified proteins as vaccines against peach allergy

January 20, 2014
Modified proteins as vaccines against peach allergy

A research conducted by the Centre for Plant Biotechnology and Genomics (UPM-INIA) and led by Araceli Díaz Perales has studied the allergy to peach, the most common food allergy, and the Pru p 3 protein. As a result of this research work, they have developed three hypoallergenic variants of this protein. All can be good candidates for the usage of specific immunotherapy for peach allergy and also can be used as a vaccine.

Nowadays, allergy affects over 25% of the population of developed countries. Currently, the treatment of food allergy consists of avoiding the intake of the food causing the allergy. However, the possibility of cross-reactivity (reaction to related food) might be ineffective.

The specific immunotherapy is the only treatment to prevent more severe signs of allergy progression. Immunotherapy consists of the intake of growing doses of allergen extracts in affected patients. However, the use of this extract could induce anaphylactic reactions or lead to sensitization to new allergens found in the mixture of allergen extracts. According to this, the usage of hypoallergenic molecules (with lower ability of binding antibodies) but with the ability to stimulate the , would be a useful tool for immunotherapy.

The most common in Spain and the Mediterranean areas is the peach allergy which is mainly caused by the Pru p 3 proteins. The current treatment of this allergy consists of avoiding peach consumption, neither fresh nor processed peaches (juices jam). As an alternative, this allergy research has defined the regions of this allergenic that is involved in the antibody binding and the stimulation of the immune system cells. After that, researchers developed three hypoallergenic variants of this protein that can be used as a .

These variants are the result of modifying epitopes (regions of binding antibodies) of this protein and they were used in a research with patient allergic to peach in order to confirm its capacity as immune system stimulation. Each variant has a different modification that was designed by using genetic tools. Although the 1 variant (Pru p 3.01) showed quite similar allergenic activity with the natural protein, the variants Pru p 3.02 and Pru p 3.03 presented lower capacity for binding antibodies. Also, it maintained its ability to stimulate immune system cells (lymphocytes) of the patients allergic to peach during the in vitro trials.

The results show that these two molecules (Pru p 3.02 and Pru p 3.03) could be good candidates for the usage of specific immunotherapy for allergy to peach.

This research work has established the basis to set a new strategy although it would be required further trials of these two molecules with animal to verify its effectiveness in the treatment for allergy to peach.

More information: "Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines". Clinical & Developmental Inmunology, Vol. 2013. dx.doi.org/10.1155/2013/385615

Related Stories

Merck: FDA reviewing tablet to eliminate allergy

March 27, 2013

Drugmaker Merck & Co. says federal regulators are reviewing its application to sell a new type of treatment for grass pollen allergy that gradually reduces allergy symptoms over time, rather than just temporarily relieving ...

Merck says FDA reviewing its ragweed allergy therapy

May 8, 2013

Drugmaker Merck & Co. says the Food and Drug Administration is reviewing its second application to sell a new type of allergy treatment meant to gradually reduce allergic reactions over time, rather than just relieving sneezing, ...

You can have a food allergy, and eat it too

November 8, 2013

Have food allergies? If you answered yes, you know the best way to prevent a severe allergic reaction is to totally avoid the offending food. But according to a presentation at the Annual Scientific Meeting of the American ...

Recommended for you

How to become a T follicular helper cell

July 30, 2015

Follicular helper Tcells (TFH cells), a rare type of immune cell that is essential for inducing a strong and lasting antibody response to viruses and other microbes, have garnered intense interest in recent years but the ...

Uncovering the secrets of immune system invaders

July 20, 2015

The human immune system is a powerful and wonderful creation. If you cut your skin, your body mobilizes a series of different proteins and cells to heal the cut. If you are infected by a virus or bacteria, your immune system ...

The role of the microbiota in preventing allergies

July 10, 2015

The human body is inhabited by billions of symbiotic bacteria, carrying a diversity that is unique to each individual. The microbiota is involved in many mechanisms, including digestion, vitamin synthesis and host defense. ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.