Mouse study points to potentially powerful tool for treating damaged hearts

Mouse study points to potentially powerful tool for treating damaged hearts

A type of cell that builds mouse hearts can renew itself, Johns Hopkins researchers report. They say the discovery, which likely applies to such cells in humans as well, may pave the way to using them to repair hearts damaged by disease—or even grow new heart tissue for transplantation.

In a study to be published in an upcoming issue of the journal eLife, the scientists also found that during , these so-called cardiac progenitor cells (CPCs) multiply without becoming heart cells in a cellular environment known as the second pharyngeal arch. This insight into the biology of CPCs may contribute to better understanding of how to prevent and treat , they say.

"Our finding that CPCs are self-renewing—that they can keep dividing to form new CPCs—means they might eventually be maintained in a dish and used to make specific types of heart cells," says Chulan Kwon, Ph.D., an assistant professor of cardiology and member of the Institute for Cell Engineering at the Johns Hopkins University School of Medicine. "Growing such cells in a dish would be an enormous step toward better treatment for ."

Kwon's research group's first step was figuring out the role of two genes, Numb and Numbl, in CPCs, which others' studies had shown are needed for guiding stem and to their fully mature, specialized functions. Numb and Numbl are highly conserved, meaning that they're nearly identical in mice, humans and other animals, a sign that they're likely very important. To find out whether these genes are required for heart formation, the group disabled Numb and Numbl in early CPCs in developing . "The embryos failed to develop normal hearts and died at an early stage of development, showing us that Numb and Numbl are needed for CPCs to build the heart," Kwon says.

This video is not supported by your browser at this time.
This shows heart tissue grown in a dish from mouse cardiac progenitor cells (CPCs). The CPCs, and the tissue they built, were engineered to produce a red protein. Credit: C. Kwon lab/eLife

The researchers next set out to find where CPCs live in the developing embryo. Using from mouse embryos, they again disabled Numb and Numbl while also engineering the cells to produce a glowing red protein, which would give away the CPCs' location. But because the engineered stem cells alone wouldn't grow into a viable embryo, the team injected them into normal mouse blastocysts—a structure formed in the early stage of mammalian development that forms both the embryo and placenta. "The normal cells in these blastocysts compensated for those that lacked Numb and Numbl, allowing the resulting embryos to survive," Kwon says.

When the team checked the hearts of the embryos, they found the glowing red cells in the second pharyngeal arch, which is known for forming parts of the neck and face. Kwon says theirs is the first study to identify it as home to CPCs. His team took cells surrounding CPCs from this arch and grew them with CPCs in a dish. They found that the CPCs self-renewed without developing into specialized . This is an important step, he says, toward using CPCs to treat heart disease.

The next step, he says, is to coax the lab-grown CPCs to form new that could be used to regenerate disease-damaged heart tissue. "Eventually, we might even be able to deliver to damaged hearts to repair heart disease," Kwon says.

More information: elifesciences.org/content/earl… 14/04/23/eLife.02164

add to favorites email to friend print save as pdf

Related Stories

Stem cells aid heart regeneration in salamanders

Apr 29, 2014

Imagine filling a hole in your heart by regrowing the tissue. While that possibility is still being explored in people, it is a reality in salamanders. A recent discovery that newt hearts can regenerate may ...

Diseased cartilage harbors unique migratory progenitor cells

Apr 02, 2009

A new study finds previously unidentified fibrocartilage-forming progenitor cells in degenerating, diseased human cartilage, but not in cartilage from healthy joints. The research, published by Cell Press in the April 3rd ...

Recommended for you

Growing a blood vessel in a week

9 hours ago

The technology for creating new tissues from stem cells has taken a giant leap forward. Three tablespoons of blood are all that is needed to grow a brand new blood vessel in just seven days. This is shown ...

Testing time for stem cells

12 hours ago

DefiniGEN is one of the first commercial opportunities to arise from Cambridge's expertise in stem cell research. Here, we look at some of the fundamental research that enables it to supply liver and pancreatic ...

Team finds key signaling pathway in cause of preeclampsia

Oct 23, 2014

A team of researchers led by a Wayne State University School of Medicine associate professor of obstetrics and gynecology has published findings that provide novel insight into the cause of preeclampsia, the leading cause ...

Rapid test to diagnose severe sepsis

Oct 23, 2014

A new test, developed by University of British Columbia researchers, could help physicians predict within an hour if a patient will develop severe sepsis so they can begin treatment immediately.

User comments