Scientists hit 'delete': Removing regions of shape-shifting protein explains how blood clots

SLU scientists hit 'delete': Removing regions of shape-shifting protein explains how blood clots
Kringles, twisted Scandinavian pastries, were the inspiration for naming the looped sections of proteins called kringle domains. Saint Louis University researcher Enrico Di Cera, M.D., examines kringle domains in an important blood-clotting protein. Credit: Riya Anandwala / Saint Louis University

In results recently published in Proceedings of the National Academy of Sciences (PNAS), Saint Louis University scientists have discovered that removal of disordered sections of a protein's structure reveals the molecular mechanism of a key reaction that initiates blood clotting.

Enrico Di Cera, M.D., chair of the Edward A. Doisy department of biochemistry and molecular biology at Saint Louis University, studies thrombin, a key vitamin K-dependent blood-clotting , and its inactive precursor prothrombin (or coagulation factor II).

"Prothrombin is essential for life and is the most important clotting factor," Di Cera said. "We are proud to report that our lab here at SLU has finally succeeded in crystallizing prothrombin for the first time."

Blood-clotting has long ensured our survival, stopping blood loss after an injury. However, when triggered in the wrong circumstances, clotting can lead to debilitating or fatal conditions such as a heart attack, stroke or .

Before thrombin becomes active, it circulates throughout the blood in the inactive (zymogen) form called prothrombin. When the active enzyme is needed (after a vascular injury, for example), the coagulation cascade is initiated and prothrombin is converted into the active enzyme thrombin that causes blood to clot.

Scientists hit 'delete': Removing regions of shape-shifting protein explains how blood clots
Using x-ray crystallography, SLU researchers published the first image of the important blood-clotting protein prothrombin (coagulation factor II). The protein’s flexible structure is key to the development of blood-clotting.

X-ray crystallography is one tool in scientists' toolbox for understanding processes at the molecular level. It offers a way to obtain a "snap shot" of a protein's structure.

In this technique, scientists grow crystals of the protein they want to study, shoot x-rays at them and record data about the way the rays are scattered by crystals. Then they use computer programs to create an image of the protein based on that data.

Once scientists can visualize the three dimensional structure of a molecule, they can begin to piece together the way in which the protein functions and interacts with other molecules in the body, or with drugs.

Last year, Di Cera and colleagues published the first structure of prothrombin. This first structure lacked a domain responsible for interaction with membranes and certain other sections were not detected by x-ray analysis. Though the scientists were able to crystallize the protein, there were disordered regions in the structure that they could not see.

Within prothrombin there are two kringle domains (looped sections of a protein named after the Scandinavian pastry) connected by a "linker" region that intrigued the SLU investigators because of its intrinsic disorder.

"We deleted this linker and crystals grew in a few days instead of months, revealing for the first time the full architecture of prothrombin," Di Cera said.

In addition to this remarkable discovery, Di Cera and colleagues found that the deleted version of prothrombin is activated to thrombin much faster than the intact prothrombin. The structure without the disordered linker is in fact optimized for conversion to thrombin and reveals key information on the mechanism of prothrombin activation.

For over four decades, scientists have tried to crystallize prothrombin but without success.

"It took us almost two years to discover that the disordered linker was the key," Di Cera said. "Finally, prothrombin revealed its secrets and with that the of a key reaction of finally becomes amenable to rational drug design for therapeutic intervention."

More information: Nicola Pozzi, Zhiwei Chen, Leslie A. Pelc, Daniel B. Shropshire, and Enrico Di Cera, The linker connecting the two kringles plays a key role in prothrombin activation, PNAS 2014 ; published ahead of print May 12, 2014, www.pnas.org/content/early/201… /1403779111.abstract

add to favorites email to friend print save as pdf

Related Stories

AB blood type strong risk factor for venous blood clots

Feb 04, 2013

The non-O ABO blood type is the most important risk factor for venous thromboembolism (blood clots in veins), making up 20% of attributable risk for the condition, according to a new study in CMAJ (Canadian Medical Associati ...

Recommended for you

A better way to track emerging cell therapies using MRIs

Sep 19, 2014

Cellular therapeutics – using intact cells to treat and cure disease – is a hugely promising new approach in medicine but it is hindered by the inability of doctors and scientists to effectively track the movements, destination ...

User comments