Association between virus, bladder cancers detected

September 11, 2013 by Stephen P Wampler, Lawrence Livermore National Laboratory
Livermore Lab biologist Crystal Jaing prepares a Microbial Detection Array slide, the primary detection technology used in an international study of bladder cancer samples.

A Lawrence Livermore National Laboratory (LLNL)-developed biological detection technology has been employed as part of an international collaboration that has detected a virus in bladder cancers.

The research, performed in conjunction with scientists from the University of Split in Croatia, LLNL and the University of Jordan in Amman is believed to be the first study to demonstrate an association between Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8, and bladder cancers.

The team's paper was published last month in the journal Tumor Biology, a publication of the International Society of Oncology and BioMarkers. Janos Terzic of the University of Split was the paper's lead author. The team included two researchers from LLNL—Crystal Jaing, a biologist in LLNL's Biosciences and Biotechnology Division, and Kevin McLoughlin, a computational biologist in LLNL's Computation Directorate.

Biopsy specimens were collected from a total of 55 Croatian patients—44 men and 11 women—who had been diagnosed with different stages of bladder cancer.

An initial screening of DNA extracts from three randomly selected biopsy specimens for the presence of viruses, using the Lawrence Livermore Microbial Detection Array (LLMDA), revealed that all three had the KSHV pathogen.

The Livermore results for the three tested samples were confirmed through KSHV-specific (PCR) testing. Then PCR tests were performed on the remaining 52 biopsy specimens from the study group. Those tests showed that KSHV DNA was detected in 30 of the 55 patients, or 55 percent of the group.

This one-inch wide by three-inch long Lawrence Livermore Microbial Detection Array contains 388,000 probes used to detect viruses and bacteria.

"We're pleased that the LLMDA performed well in the testing of the bladder cancer samples," Jaing said. "For us, it is the first publication showing the technology used as the primary detector of a virus associated with a specific disease, which was then confirmed using other techniques."

In their paper, the authors noted that with the high prevalence of KSHV infection demonstrated in their study, the pathogen may play a role in the formation of bladder cancer, and warrants further study.

Bladder cancer is the seventh most common human malignancy and represents a global health problem. In addition to recognized risk factors, such as smoking and exposure to chemicals, various infectious agents also have been viewed as factors in the causes of the disease.

Developed between October 2007 and February 2008, the LLMDA detects viruses and bacteria with the use of 388,000 probes that fit in a checkerboard pattern in the middle of a one-inch-wide, three-inch-long glass slide.

The current operational version of the LLMDA contains probes that can detect about 3,100 viruses and about 2,000 bacteria. The array also encompasses fungi and protozoa—with probes representing 136 fungi and 94 protozoa. Analysis of samples with the LLMDA can be completed within 24 hours.

In 2010, the LLMDA was used in a research study by scientists from five institutions who evaluated the DNA content of eight vaccines. Seven of the vaccines' contents turned out as expected, but one—a vaccine used to prevent diarrhea in babies—contained a pig virus, porcine circovirus-1 (PCV-1). There have been no signs of safety problems with the vaccine and the virus is not known to cause any kind of illness in people or animals.

Explore further: 'Scent device' could help detect bladder cancer

More information: link.springer.com/article/10.1 … 07/s13277-013-1079-2

Related Stories

'Scent device' could help detect bladder cancer

July 8, 2013
Researchers from the University of Liverpool and University of the West of England, (UWE Bristol), have built a device that can read odours in urine to help diagnose patients with early signs of bladder cancer.

Study identifies trigger for alternate reproduction of HIV-related cancer virus

April 17, 2012
A research team led by Children's National Medical Center has identified a trigger that causes latent Kaposi's sarcoma-associated herpesvirus (KSHV) to rapidly replicate itself. KSHV causes Kaposi's sarcoma, primary effusion ...

Scientists make new discoveries in the transmission of viruses between animals and humans

August 12, 2013
Outbreaks such as the severe acute respiratory syndrome (SARS) Middle East respiratory syndrome coronavirus (MERS) have afflicted people around the world, yet many people think these trends are on the decline.

Recommended for you

How cancer metastasis happens: Researchers reveal a key mechanism

January 18, 2018
Cancer metastasis, the migration of cells from a primary tumor to form distant tumors in the body, can be triggered by a chronic leakage of DNA within tumor cells, according to a team led by Weill Cornell Medicine and Memorial ...

Modular gene enhancer promotes leukemia and regulates effectiveness of chemotherapy

January 18, 2018
Every day, billions of new blood cells are generated in the bone marrow. The gene Myc is known to play an important role in this process, and is also known to play a role in cancer. Scientists from the German Cancer Research ...

These foods may up your odds for colon cancer

January 18, 2018
(HealthDay)—Chowing down on red meat, white bread and sugar-laden drinks might increase your long-term risk of colon cancer, a new study suggests.

The pill lowers ovarian cancer risk, even for smokers

January 18, 2018
(HealthDay)—It's known that use of the birth control pill is tied to lower odds for ovarian cancer, but new research shows the benefit extends to smokers or women who are obese.

Researchers develop swallowable test to detect pre-cancerous Barrett's esophagus

January 17, 2018
Investigators at Case Western Reserve University School of Medicine and University Hospitals Cleveland Medical Center have developed a simple, swallowable test for early detection of Barrett's esophagus that offers promise ...

Scientists zoom in to watch DNA code being read

January 17, 2018
Scientists have unveiled incredible images of how the DNA code is read and interpreted—revealing new detail about one of the fundamental processes of life.

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.