Stressed dads affect offspring brain development through sperm microRNA

Penn: Stressed dads affect offspring brain development through sperm microRNA
University of Pennsylvania researchers have shown at the molecular level how experiencing stress changes a male mouse's sperm in such a way that it affects his offspring's response to stress. This change is imparted epigenetically, or through a means other than the DNA code, by molecules called microRNAs, or miRs. Credit: University of Pennsylvania

More and more, scientists have realized that DNA is not the only way that a parent can pass on traits to their offspring. Events experienced by a parent over a lifetime can also have an impact.

Now University of Pennsylvania researchers have shown at the molecular level how experiencing stress changes a 's sperm in such a way that it affects his offspring's response to stress. This change is imparted epigenetically, or through a means other than the DNA code, by molecules called microRNAs, or miRs.

The work, led by Tracy L. Bale, professor of neuroscience in Penn's School of Veterinary Medicine and Perelman School of Medicine, provides important clues for understanding how a father's life experiences may affect his children's brain development and through a purely biological and not behavioral means.

"It's remarkable to me that seemingly mild stress to a male mouse would trigger this massive change in microRNA response and that that would get wired into the course of his offspring's development," Bale said.

She collaborated on the work with graduate students Ali B. Rogers and Christopher P. Morgan and research specialist N. Adrian Leu of Penn Vet. The paper will appear in Proceedings of the National Academy of Sciences.

In earlier research, Bale's lab had shown that male mice that were stressed, prior to being bred, by such means as changing cages or exposing them to a predator odor of fox urine, had offspring with a dampened response to stress. When they compared sperm from the stressed fathers to their unstressed counterparts, they found increased expression of nine miRs in the stressed animals. Unlike some other types of RNA, miRs do not code for a protein; instead, they serve to silence or degrade specific messenger RNAs, preventing them from being translated into proteins.

"Just showing that the levels were different doesn't make it relevant or interesting," Bale said. "We wanted to find out whether they were having a causal role."

To find out, the team microinjected the nine miRs into mouse zygotes, which were then implanted into normal female mice who carried them as surrogates. They also included control groups in which zygotes received either a sham injection or an injection of a single miR. When the offspring became adults, the researchers examined their response to stress, just as they had done in their 2013 study.

"The results mapped right onto what we had shown before," Bale said.

When subjected to a mild stress, in this case, being restrained briefly, the offspring that arose from the zygotes that received the multi-miR injections had lower cortisone levels compared to offspring in the control groups. The mice in the multi-miR injection group also had significant changes in the expression of hundreds of genes in the paraventricular nucleus, a brain region involved in directing stress regulation, suggesting wide-spread changes in early neurodevelopment.

Finally, the researchers aimed to determine how the miRs were carrying out this effect after fertilization. Because miRs are known to target and degrade mRNA, the team looked at the stored maternal mRNA, a genetic bundle that is contained in the egg when it is fused with the sperm and exists for only a brief window of time to direct early zygotic development.

"People used to think that because that stored maternal mRNA gets translated during that initial two-cell and four-cell development, the mom gets a lot of say in those early stages and the dad gets no say," Bale said. "But we thought maybe these sperm miRs could be attacking that maternal mRNA and directing which mRNAs get translated."

The researchers again injected miRs into zygotes and performed control injections, but this time they incubated the zygotes for eight hours and then amplified the RNA in each single cell to look for gene expression levels. They found that, indeed, the multi-miR injection appeared to be attacking the maternal mRNA, resulting in a reduction in those mRNA levels compared to control injections. Specifically affected were genes involved in chromatin remodeling.

Bale suspects that when a male experiences stress it may trigger the release of miRs contained in exosomes from the epithelial cells that line the epididymis, the storage and maturation site for sperm between the testes and the vas deferens. These miRs may be incorporated into the maturing sperm and influence development at fertilization.

Up next for the group, including Penn Vet graduate student Jen Chan, who is taking over the project, is to examine what upstream factors could lead to exosome and miR release and whether an intervention, such as providing stressed males with enrichment or a reward, might prevent them from passing on an abnormal stress response to the next generation.

They also hope to study the role of miRs in humans to discern whether some may vary in response to in a similar way as in mice.

The work was supported by the National Institute of Mental Health.


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Dad's life stress exposure can affect offspring brain development, study finds

More information: Transgenerational epigenetic programming via sperm microRNA recapitulates effects of paternal stress, PNAS, www.pnas.org/cgi/doi/10.1073/pnas.1508347112
Citation: Stressed dads affect offspring brain development through sperm microRNA (2015, October 19) retrieved 22 July 2019 from https://medicalxpress.com/news/2015-10-stressed-dads-affect-offspring-brain.html
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JVK
Oct 19, 2015
"It's remarkable... that seemingly mild stress ... would trigger this massive change in microRNA response and that that would get wired into the course of his offspring's development," Bale said.


It is remarkable to me that Bale did not recognize the obvious link from The epigenetics of sex differences in the brain. http://www.ncbi.n...19828794

We reported the obvious link in the context of the molecular epigenetics section of our 1996 Hormones and Behavior review article: From Fertilization to Adult Sexual Behavior http://www.hawaii...ion.html

It's not just the microRNAs in sperm, it's the difference in the viral microRNAs and nutrient-dependent microRNAs that links the transgenerational epigenetic inheritance of virus-induced pathology or nutrient-dependent healthy longevity via RNA-mediated gene duplication and RNA-mediated amino acid substitutions that stabilize organized genomes.

Oct 20, 2015
Cool, does this mean that the more stressed people are the more laid back the next generation. ;-)

Oct 23, 2015
The researchers caused stress-induced changes at an early stage of embryonic development with microRNA injections. The resultant adverse effects weren't observed until the subjects were adults. This study should inform those who restrict their view of causes for adverse effects to only postnatal environments and events.

The transgenerational epigenetic mechanism of microRNA differed from a study that showed how treating the father's symptoms of an inherited disease can epigenetically treat the son. The mechanism researched in that study was DNA and histone methylation. This study should inform those who restrict their view of epigenetic changes to only DNA methylation.

There's also the point of most of the news coverage focus, that it was a male's rather than a female's stress that affected the developing embryo.

http://surfaceyou...ealself/

JVK
Oct 23, 2015
Nutrient-dependent pheromone-controlled ecological adaptations: from atoms to ecosystems
http://figshare.c...s/994281

"This atoms to ecosystems model of ecological adaptations links nutrient-dependent epigenetic effects on base pairs and amino acid substitutions to pheromone-controlled changes in the microRNA / messenger RNA balance and chromosomal rearrangements. The nutrient-dependent pheromone-controlled changes are required for the thermodynamic regulation of intracellular signaling, which enables biophysically constrained nutrient-dependent protein folding; experience-dependent receptor-mediated behaviors, and organism-level thermoregulation in ever-changing ecological niches and social niches. Nutrient-dependent pheromone-controlled ecological, social, neurogenic and socio-cognitive niche construction are manifested in increasing organismal complexity in species..."

Oct 23, 2015
Nutrient-dependent pheromone-controlled ecological adaptations: from atoms to ecosystems
http://figshare.c...s/994281

Criticisms of the nutrient-dependent pheromone-controlled evolutionary model
http://www.ncbi.n...4049134/

"Kohl demonstrates a blatant disregard for established nomenclature."

"Based on his writings, both published and unpublished, James Kohl presents an unsupported challenge to modern evolutionary theory and misrepresentations of established scientific terms and others' research."

JVK
Oct 23, 2015
Re:
"Kohl demonstrates a blatant disregard for established nomenclature."


So does every other serious scientist. The established nomenclature was established by biologically uninformed science idiots, and some of them are still trying to use it to link mitochondrial mutations to biodiversity.

See: Genetics probe identifies new Galapagos tortoise species http://www.nature...-1.18611

Re:
James Kohl presents an unsupported challenge to modern evolutionary theory and misrepresentations of established scientific terms and others' research.


See the parody. There is nothing left of what anonymous fools let themselves be taught to believe in.

https://www.youtu...youtu.be All About that Base (Meghan Trainor Parody) 10 Dec 2014

The whole world of serious scientists is laughing at evolutionary theorists. Why not join us in the real world?

JVK
Oct 23, 2015
A virocentric perspective on the evolution of life http://www.scienc...13001028

• The existence of hallmark genes implies that viruses and related selfish elements evolved from the primordial gene pool.

Creationists claim that viruses steal the energy that cells need to support thermodynamic cycles of protein biosynthesis and degradation that link the epigenetic landscape to the physical landscape of DNA in all cell types of all living genera via their physiology of reproduction.

Phys,org removes any of my posts that contain links to the creationist's literature.

The biologically uninformed science idiots here think they can make claims based on less than half of what is currently known about the difference between healthy longevity and virus-driven pathology.

See instead: https://www.youtu...G_9EEeeA

Oct 23, 2015
"The Bible, consisting of the thirty-nine canonical books of the Old Testament and the twenty-seven canonical books of the New Testament, is the divinely-inspired revelation of the Creator to man. Its unique, plenary, verbal inspiration guarantees that these writings, as originally and miraculously given, are infallible and completely authoritative on all matters with which they deal, free from error of any sort, scientific and historical as well as moral and theological."

This is from the site JVK cries PO won't let him link to.

There's more.

"The eventual accomplishment of God's eternal purposes in creation, with the removal of His curse and the restoration of all things to divine perfection, will take place at the personal bodily return to earth of Jesus Christ to judge and purge sin and to establish His eternal kingdom."

JVK's choice of links is another indication of his lack of credibility.


JVK
Oct 23, 2015
JVK's choice of links is another indication of his lack of credibility.


The anonymous fool Vietvet (aka Steven Taylor) provides no links.

See also: The Bull Sperm MicroRNAome and the Effect of Fescue Toxicosis on Sperm MicroRNA Expression http://dx.doi.org....0113163

Everything known about nutrient-dependent RNA-mediated cell type differentiation in soil bacteria is linked via the biophysically constrained chemistry of plants to the physiology of reproduction in all invertebrates and invertebrates by the conserved molecular mechanisms we detailed in the molecular epigenetics section of our 1996 review. From Fertilization to Adult Sexual Behavior http://www.hawaii...ion.html

See also: "The Darwin Code: Intelligent Design without God" http://rna-mediat...eg-bear/

The issues here are not about religion; they are about information dissemination.

Oct 24, 2015
The quotes I posted came from The Institute For Creation Research, the site PO barred JVK from linking.

JVK
Oct 24, 2015
PO prevented me from linking to an article with the title: "Viral Genome Junk Is Bunk" and claimed that the article was PSEUDOSCIENCE.

See instead: Viral and Cellular Genomes Activate Distinct DNA Damage Responses
http://dx.doi.org...5.07.058

Excerpt:"...γH2AX foci discriminate "self" and "non-self" genomes and determine whether a localized anti-viral or global ATM response is appropriate. This provides an elegant mechanism to neutralize viral genomes without jeopardizing cellular viability."

See also: Akt Kinase-Mediated Checkpoint of cGAS DNA Sensing Pathway http://dx.doi.org...5.09.007

Excerpt: "OAS proteins limit viral propagation through the activation of the enzyme RNase L, which degrades host and viral RNA. 2′-5′-linked cGAMP activates downstream signaling pathways to induce de novo antiviral gene expression (Hornung et al., 2014)."

Steven Taylor (aka Vietvet) is a biologically uninformed science idiot!

JVK
Oct 24, 2015
Re: "...de novo antiviral gene expression..."

The term "de novo" is consistently used by theorists to indicate that they have no ideas about how to link biologically-based cause and effect from atoms to ecosystems.

"Magic of evolution" and de novo are interchangeable in theories. "Viruses are linked to all pathology" and "Control of pathology is nutrient-dependent" are the proven claim of creationists.

Oct 25, 2015
Phys,org removes any of my posts that contain links to the creationist's literature
[sic]
@jk
that is because of two things:
1- there is NO SCIENCE in the creationist religion: when you actively attempt to manipulate your results in order to attempt to justify a belief or a comic book, be it manga, novella or the bible, then it is called PSEUDOSCIENCE

2- https://en.wikipe...Arkansas

when you link this site as well as other creationist literature on your personal sites, they become PSEUDOSCIENCE religious sites

and because you personally own and control them, they also are PHISHING sites capable of mining personal info from visitors
reported

Oct 25, 2015
"Viruses are linked to all pathology" and "Control of pathology is nutrient-dependent" are the proven claim of creationists.
@jk
so they're proven?
all pathology is virus linked?
Pathology (from the Ancient Greek roots of pathos (πάθος), meaning "experience" or "suffering", and -logia (-λογία), "an account of") is a significant component of the causal study of disease and a major field in modern medicine and diagnosis.
The term pathology itself may be used broadly to refer to the study of disease in general, incorporating a wide range of bioscience research fields and medical practices (including plant pathology and veterinary pathology), or more narrowly to describe work within the contemporary medical field of "general pathology," which includes a number of distinct but inter-related medical specialties which diagnose disease mostly through the analysis of tissue, cell, and body fluid samples
https://en.wikipe...athology

2Bcont'd

Oct 25, 2015
@jk con't
Viruses are linked to all pathology
so... radiation poisoning is virus driven?
how about heavy metal poisoning?
from the above link
Forensic pathology focuses on determining the cause of death by post-mortem examination of a corpse or partial remains
so, per your "proven claim[s] of creationists", there is a virus that kills everything, thus we only need to do a blood/fluid check for an autopsy, correct?
per your claim
Viruses are linked to all pathology
e-coli cannot kill, and neither can any other bacteria, be it MRSA or any other...

but we already know that isn't true just be watching CSI,, Law and Order, NCIS, or any other crime show on TV, so, by logical extrapolation, we can thus conclude, you are either:
1- drunk
2- delusional
3- mentally impaired
4- stupid
5- all of the above

take your pick
http://www.dpa.st...h17.html

JVK
Oct 25, 2015
so, per your "proven claim[s] of creationists", there is a virus that kills everything


I wrote: "Viruses are linked to all pathology" and "Control of pathology is nutrient-dependent" are the proven claim of creationists.

I did not claim
there is a virus that kills everything
Supercoiled DNA protects organized genomes against entropy or there would be no reason for serious scientists to promote precision medicine, which links nutritional epigenetics to pharmacogenomic in the context of models that link atoms to ecosystems.

Clinically Actionable Genotypes Among 10,000 Patients With Preemptive Pharmacogenomic Testing http://www.medsca...24253661

Oct 25, 2015
I did not claim
lets examine that statement again, shall we?
start with the definition of pathology: https://en.wikipe...athology

it's quoted above: now, when you state
"Viruses are linked to all pathology"
[sic]
so, per your words, all pathology is virus linked (simply reworded above, but exact same message)
thus, per your own words, Any and all pathology has a viral link to it, so that any hostile deadly death MUST BE, by logical extrapolation of your wording, viral

this is the problem with your lack of literacy, reading, comprehension, Dunning-Kruger, and more: you make grand assumptions that are fallacious (means you lie like a turd in a toilet bowl) and then assume everyone will accept it because "you're someone special"

you've never brought evidence proving all pathology is viral. not one bit!
just a claim.
and a completely stupid one considering the definition of pathology
2Bcont'd

Oct 25, 2015
@jk cont'd
you can't even say that you meant that all viruses are pathological, because that would infer that either: all viruses are deadly (they're not- everyone on earth is routinely exposed to viruses and not dead from them. for instance: the common cold, or the flu)
OR
they would think you meant that all types of pathology are virus driven (which we discussed above, and that is proven to be false with the link provided)

Thus, we can see, because you have previously stated
I don't use definitions ...
JUN 26 http://phys.org/n...ion.html

this is one reason you couldn't pass a college course, because it requires you to learn the BASICS, which are important: like terminology, definitions

refusing them means you will always be a pseudoscience crackpot posting religion over science

reported

JVK
Oct 25, 2015
requires you to learn the BASICS, which are important: like terminology, definitions


What definitions are used in this insulting parody that integrates what is known to serious scientists about base pairs and cell type differentiation?

https://www.youtu...youtu.be All About that Base (Meghan Trainor Parody)

What is your definition of a retrotransposon? Can you place the definition into the context of this article "MIR retrotransposon sequences provide insulators to the human genome" http://www.pnas.o...abstract

JVK
Oct 26, 2015
See also: Phobias may be memories passed down in genes from ancestors
http://www.telegr...ors.html

This can be addressed in the context of reports on "snake-centric" evolution of the human brain. If your ancestors were not eaten, their brains evolved better detection systems.

"Isbell calls these findings "the first neuroscientific support" for her snake-centric evolutionary theory." excerpted from http://news.scien...te-brain

See my comment: "The context of adaptive evolution in which the spontaneous binding between congruent olfactory and visual information forms a multimodal saliency map in species with adaptively evolved eyes and individual humans who are not congenitally blind has been removed.

Instead, in two primates, the saliency of the visual input is automagically established outside the context..."

JVK
Oct 26, 2015
See for comparison: How we remember what we can do
http://www.socioa...ew/24807

Excerpt: "...neuropsychological data demonstrating that the visual perception of an object automatically triggers some activity in the motor areas (especially when the object is located in the peripersonal space) does not necessarily mean that a data processing mechanism is simulating an action directed to the object with the purpose of determining its feasibility in current circumstances, i.e. 'provide the self with information on the feasibility of action potentials' (Coello & Delevoye-Turrell, 2007)."

See also: Nutrient-dependent/pheromone-controlled adaptive evolution: a model. http://www.ncbi.n...24693353 "The honeybee already serves as a model organism for studying human immunity, disease resistance, allergic reaction, circadian rhythms, antibiotic resistance, the development of the brain and behavior, mental health..."

Oct 26, 2015
blah blah adaptive evolution: a model
which has only ONE cite, here: http://www.ncbi.n...4049134/

and said cite also falsifies your model
What definitions are used in this insulting parody
continuing to link the OT parody because you can't answer questions or demonstrate validated scientific data while continuing to post religious dogma? is this because you are outed as a chronic liar?
perhaps you should read this:
https://www.psych...ttle-ego

What is your definition of a retrotransposon?
since you are too illiterate to actually utilise a search engine or open a dictionary:
https://en.wikipe...ansposon

Can you place the definition into the context of this article
please demonstrate that YOU can so we can determine if your comprehension skills have degraded or are remaining the same WRT interpretations of science
thanks!

JVK
Oct 26, 2015
Thanks for taking the bait! YOU MORON!

Others, see: https://en.wikipe...ansposon

"The retrotransposons' replicative mode of transposition by means of an RNA intermediate rapidly increases the copy numbers of elements and thereby can increase genome size."

The retrotransposons link nutrient-dependent RNA-directed DNA methylation to RNA-mediated gene duplication and the RNA-mediated experience-dependent de novo gene creation linked directly fron odor exposure to cell type differentiation in all cell types of all individuals of all living genera via the physiology of their biophysically constrained nutrient-dependent reproduction and mutation-driven loss of genes that are not required for species survival.

which has only ONE cite, here: http://www.ncbi.n...4049134/


You fool! Every serious scientist knows about this citation to my work: http://www.scienc...14004006

JVK
Oct 26, 2015
Criticisms of the nutrient-dependent pheromone-controlled evolutionary model http://www.ncbi.n...4049134/
cites:

Darwin C. On the origin of species. 1st ed. 1859. p. 61. [Online forum comment]. Retrieved March 10, 2014 from http://darwin-onl...eseq=76.
Kohl J. V. Mammalian olfactory-genetic-neuronal-hormonal-behavioral reciprocity and human sexuality. Sarasota, FL: Association for Chemoreception Sciences; 1996.
Kohl J. V. Human pheromones and food odors: epigenetic influences on the socioaffective nature of evolved behaviors. Socioaffective Neuroscience & Psychology. 2012;2:17338. [PMC free article] [PubMed]
Kohl J. V. Nutrient-dependent/pheromone-controlled adaptive evolution: a model. Socioaffective Neuroscience & Psychology. 2013a;3:20553. [PMC free article] [PubMed]

[cont]

JVK
Oct 26, 2015
[cont]

Kohl J. V. Evolutionary psychology; 2013b. Jun 29, [Online forum comment]. Retrieved March 10, 2014 from https://groups.ya.../150648.
Kohl J. V. A third of Americans don't believe in evolution; 2014a. Jan 4, [Online forum comment]. Retrieved March 10, 2014 from http://phys.org/n...on.html.
Kohl J. V. One crank dies, another rises to take his place [Blogpost comment]; Pharyngula; 2014b. Jan 7, Retrieved March 10, 2014 from http://freethough...-page-1/
Nei M. Mutation-driven evolution; 2013. Retrieved March 10, 2014 from books.google.com.
Prakash M. Encyclopaedia of gene evolution. New Delhi: Discovery Publishing House; 2006.

No experimental evidence of biologically-based cause and effect is included by the anonymous fool (aka anonymous_9001), who seems to think that genes evolve.

Oct 26, 2015
this citation to my work
@jk
1- THAT citation is not your model, which has NO citations
2- making a claim that your work is cited but linking a pay-walled study which cannot verify the claim is like telling us you are a diagnostician while not being able to prove it with a license
Thanks for taking the bait
thank you for demonstrating you still can't comprehend WTF the scientific method is, or how to validate a claim
Kohl J. V
posting your publication history is a fallacy known as argument from authority
considering you've still not been able to actually prove Evolution Theory false
AND
any claims you have WRT your medical license
AND
that you are also proven to be a chronic liar here on PO (repeatedly, by more than just myself)
THEN
your argument from authority is not only invalid, but useless, as it doesn't prove anything
- except that you're trying to argue from false assumptions and can't produce evidence

JVK
Oct 26, 2015
2- making a claim that your work is cited but linking a pay-walled study which cannot verify the claim is like telling us you are a diagnostician while not being able to prove it with a license


Thanks again for taking the bait. Here's a link to the pdf and the quote from it.

http://www.resear...35f4.pdf

The OL acting as control centre may be target organ for metabolic hormones such as leptin like
and insulin like peptides, and olfactory organ could exert regulatory action on the OL via epigenetic effects of nutrients and pheromones on gene expression (Kohl, 2013; Elekonich and Robinson, 2000).

Elekonich and Robinson (2000) linked our 1996 Hormones and Behavior review to insects.
http://www.ncbi.n...10980296

Now you can complain about lack of access to that article, if you wish. YOU MORON!

Oct 26, 2015
for taking the bait
@the idiot kohl
for starters: this is how you should actually form an evidence based scientific argument (you have had plenty of demonstrations of that already), so it is not "taking the bait", you moron, it was "forcing you to finally demonstrate an evidence based scientific argument"

for two: at least you are actually posting a scientific reference, not a religious one. kudos for that! (i would say that you should be proud, but the law of averages stated you had to eventually do it... i am more surprised you had not done it earlier)
The OL acting as
you should have first quoted the beginning of the paragraph
To date many questions remain still open. Future work on O. vulgaris olfaction must also consider how animals acquire the odours detected by the olfactory organ and what kind of odour the olfactory organ perceives
It makes more sense that way
it also gives more context (another problem of yours... we'll deal with that later)

Oct 26, 2015
@jk cont'd
you make a LOT of claims. these claims are not usually backed by science, evidence or even logical thought. such as your claims of being a diagnostician ( http://phys.org/n...nes.html ) or being a microbiologist or that all mutations perturb protein folding ( http://medicalxpr...mic.html ) despite the evidence to the contrary (Lenski et al; Extavour et al)

Then there is the fact that you are a chronic religious fanatic posting creationist diatribe
http://phys.org/n...ies.html

http://phys.org/n...nce.html

http://phys.org/n...ane.html

http://phys.org/n...rds.html

Don't be mad when you're taken as a liar because of past performance
I will give credit to ACTUAL evidence, but not your LIES or religion/pseudoscience

Oct 26, 2015
@jk last point:
your link: http://www.resear...35f4.pdf

there is NO INDICATION WHATSOEVER that this study in ANY way usurps, refutes, undermines or debunks the Theory of Evolution

the ONLY thing that it does do is validate the claim of yours that it was cited by someone other than Mr. Jones, BA.
also
In aquatic animals ''Odours'' are molecules in solution that guide them to locate food, partners, nesting sites, and dangers to avoid. Fish, crustaceans and aquatic molluscs possess sensory systems that have anatomical similarities to the olfactory systems of land-based animals...
The reproductive behaviour of Octopus vulgaris is under the control of a complex set of signal molecules ...These signals are intercepted by the olfactory organs and integrated in the olfactory
none of this states it is effective in humans, either

Oct 26, 2015
@jk and lastly, your claims, historically, are that your "model" undermines evolution and that your "model" is equally demonstrated from humans to single celled organisms.

it doesn't

the above linked study (not your model) demonstrates "Role of olfaction in Octopus vulgaris reproduction"... it in no way demonstrates the effective application of your "model" to humans, etc per your claims

And about your "model"
until you can prove your conclusions (which you do not), and until you can reconcile your previous comments about mutations (which your "model" requires, despite your claims that they're always negatively selected for or pathological) and until you can actually find "robust bioassay-led evidence" that demonstrates human pheromones and is validated ( http://rspb.royal...full.pdf ) then you are STILL making unfounded claims with regard to your beliefs, and not evidence based conclusions

got it yet?

JVK
Oct 26, 2015
your claims, historically, are that your "model" undermines evolution...


That claim was made by Andrew Jones, you MORON. http://www.ncbi.n...4049134/ "...James Kohl presents an unsupported challenge to modern evolutionary theory..."

... and that your "model" is equally demonstrated from humans to single celled organisms.


I provided examples that linked nutrient-dependent RNA-mediated events to amino acid substitutions and ecological adaptations across species.

you are STILL making unfounded claims with regard to your beliefs, and not evidence based conclusions


What kind of biologically uninformed science idiot alters my claims and claims that my claims are unfounded? Does Captain Stumpy think everyone else here is a biologically uninformed science idiot?

See also: http://www.newyor...ur-brain What aspect of brain cell type differentiation in offspring is mutation-driven?

Oct 26, 2015
your claims, historically, are that your "model" undermines evolution...

That claim was made by Andrew Jones, you MORON


In other news, Kohl doesn't know what "undermines" means. Undermine does not mean "presents an unsupported challenge".

Oct 26, 2015
That claim...
@jk
so, are you actually trying to state that you support Evolution Theory?
or are you saying that you have never posted your "model" to define what is happening and then take the stance that it undermines Evolution?

so i really need to start linking your specific quotes and threads AGAIN?
aren't you in enough hot water for lying, posting pseudoscience and claiming to be a licensed professional without even being able to show a degree for it?
...across species.
you can't say its applicable to humans when you still can't produce "robust bioassay-led evidence" that demonstrates human pheromones exist
(which was the point of this link: http://rspb.royal...full.pdf )

alters my claims
i never alter your claims: they're stupid enough without need for that
i usually quote them VERBATIM and paraphrase at times
hence the LINKS, you idiot-girl

2Bcont'd

Oct 26, 2015
@the idiot jk cont'd
... my claims are unfounded?
as for them being unfounded... as i stated above:
this is because you've demonstrated YOURSELF to be a chronic liar, by your own words, and by your actions, and by false representation (of the science AND yourself)

the bulk of your "comments" are totally unfounded as well as being misleading or based upon religious and/or delusional beliefs that have NO bearing on reality, let alone science

and i can prove that with links/quotes, so it is not conjecture: it is an evidenciary based assessment of your historical posts

your week is ticking by...
i suggest linking the evidence required.

otherwise...

JVK
Oct 26, 2015
http://www.newyor...d-latest

Excerpt: "Our own DNA is a battlefield between self and other".

My comment: Supercoiled DNA links nutrient energy-dependent base pair changes to protection from virus-driven genomic entropy via links from nutritional epigenetics to pharmacogenomics that begin with virus-driven changes in gut microbes. The changes attributed to viral microRNAs would more quickly destroy our immune system protection if not for the contribution of nutrient-dependent microRNAs to RNA-mediated DNA repair and healthy longevity.

Evolutionary theorists would like you to think that natural section links the anti-entropic effects of nutrient-dependent microRNAs to evolution, but their claims place natural selection too far away from food selection, which is required to support the innate immune system of all cell types in all individuals of all living genera.

JVK
Oct 27, 2015
See also: Four microRNAs identified as playing key roles in cholesterol, lipid metabolism
http://medicalxpr...pid.html

Serious scientists will link yet another fact about the nutrient-dependent microRNA/messenger RNA balance from atoms to ecosystems via what is currently known about epigenetic effects on metabolic networks and genetic networks that also link transgenerational epigenetic inheritance of morphological and behavioral phenotypes via sperm microRNAs.

Theorists will continue to play the game of definitions and assumptions. They will revise their theories to make it seem as if they had the explanatory power of facts supported by experimental evidence that links the epigenetic landscape to the physical landscape of DNA in all living genera via protection against virus-driven genomic entropy.

Oct 27, 2015
Theorists will continue to play the game of definitions and assumptions. They will revise their theories to make it seem as if they had the explanatory power of facts supported by experimental evidence...
First thing: this means you don't have any clue as to how the scientific method works
https://en.wikipe...c_method

secondly, a theorist takes past known factual data and builds a hypothesis about something, then methods of how to test it to see if it is factual or not. Then, in conjunction with experimental scientists, they test said hypothesis and gather data... revise if necessary, and retest
https://en.wikipe...c_method#/media/File:The_Scientific_Method_as_an_Ongoing_Process.svg

making a statement like yours above means you are not familiar at all with science or it's methodology

your statement demonstrates the effects of your fanatical religious beliefs over science
IOW- it's delusional

Oct 27, 2015
@jk cont'd
the game of definitions and assumptions
and do i really need to address this again?

there is a typical professional lexicon / nomenclature associated with any scientific field. this is developed in order to facilitate clear, concise communication as well as enable comprehension and understanding (something that is anathema to you, apparently)

knowing the definitions of a word or the terminology of the field allows a person to not only accurately communicate with other professionals, but also with the public

failure to actually learn said lexicon or utilise it in a field demonstrates that a person is untrained, ignorant, and that said person is likely not familiar with the field (a telling marker of your pseudoscience and religious proclamations)

when you refuse to learn about your own field, why then should anyone else take you seriously?

JVK
Oct 27, 2015
See also: http://medicalxpr...ome.html

"The genes we found are called long non-coding RNAs, or LncRNAs," said Gay Crooks, co-director of the UCLA Broad Stem Cell Research Center, a member of the UCLA Jonsson Comprehensive Cancer Center and co-senior author of the study. "They make up much of what we used to think of as the 'dark matter' of our genome because, unlike the better-known messenger RNA genes, they do not produce proteins. The function of LncRNAs is not well-known but it is becoming increasingly apparent that they are not inert; they have a critical role in controlling how other genes function."

My comment: Does anyone agree that all the problems with neo-Darwinian theory can be solved by claiming that microRNAs and messenger RNAs are genes?

Oct 27, 2015
My comment:
your comment is irrelevant and OT

JVK
Oct 28, 2015
My comment can be viewed in the context of this webinar, which is available on demand.

The Hidden Effects of Epigenetics https://www.labro...nd-oct28

Although there is "No Boundary Really Between Genetic and Epigenetic" and "...evolutionary science has now "...moved on to such an extent" that she and Peter Saunders don't really care anymore about "trying to convince the neo-Darwinists." see:

http://www.huffin...450.html

-- the next generation will not be taught to believe that RNAs are genes. No one who is raised in this century will ever be as ignorant as theorists have been since they invented neo-Darwism at the turn of the 20th century.


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