MicroRNA-708 overexpression suppresses beta-cell proliferation

MicroRNA-708 overexpression suppresses β-cell proliferation

(HealthDay)—Researchers have identified a novel mechanism of glucose regulation of β-cell function and growth by repressing stress-induced microRNA-708 (miR-708), according to a study published online Oct. 2 in Diabetes.

Júlia Rodríguez-Comas, from the Institut d'Investigacions Biomèdiques August Pi i Sunyer in Spain, and colleagues examined miRNAs that are modulated by glucose in mouse pancreatic islets.

The researchers found that miR-708 was the most upregulated miRNA in islets cultured at low glucose concentrations, which triggers a strong stress response. MiR-708 was also potently upregulated by triggering endoplasmic reticulum stress with thapsigargin and in islets of ob/ob mice. Treatment with the chemical chaperone 4-phenylbutyrate blocked low-glucose induction of miR-708. Neuronatin (Nnat) was identified as a potential glucose-regulated target of miR-708 in integrative analysis.

There was an inverse correlation for Nnat expression with miR-708 in islets cultured at different and in ob/ob mouse islets; this expression was reduced after of miR-708. MiR-708 overexpression impaired glucose-stimulated insulin secretion (GSIS), which was recovered by overexpression of Nnat, consistent with the role of Nnat in the secretory function of β-cells. In islets cultured at low glucose, miR-708 inhibition recovered GSIS. Overexpression of miR-708 suppressed β-cell proliferation and induced β-cell apoptosis.

"To fully understand whether changes in the expression of this microRNA are the cause or the consequence of β-cell dysfunction in these models still requires further investigation," the authors write.


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Citation: MicroRNA-708 overexpression suppresses beta-cell proliferation (2017, October 19) retrieved 6 December 2019 from https://medicalxpress.com/news/2017-10-microrna-overexpression-suppresses-beta-cell-proliferation.html
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