Seeing isn't believing: Biologists show how to shut off hunger 'alarm system'

December 5, 2017, University of Pennsylvania
Researchers at Penn identified a connection between the gut and brain that acts to reduce an animal's drive to eat. Targeting the neurons involved in this "hunger alarm system" could provide a strategy to treat obesity. Credit: University of Pennsylvania

Imagine you're in a restaurant, hungry, anxious and a bit irritable awaiting your food order to arrive at the table. The server exits the kitchen with a tray full of steaming plates and a flood of relief washes over you. But the server ferries the food right past you to another table, and the unpleasant sensation of hunger returns—at least until you take the first bite of your very own meal.

With a new study, at the University of Pennsylvania have explained the biological mechanism behind these hunger-driven highs and lows. While the mere sight or smell of can temporarily turn off responsible for the drive to eat, they showed that the neurons only stay off if the brain receives a signal from the stomach that calories have been ingested.

"When these neurons are firing, they're basically telling you, 'You'd better go get food; you're starving,'" said J. Nicholas Betley, assistant professor in the Department of Biology in Penn's School of Arts and Sciences. "They're a sensitive alarm system. And what this study conclusively demonstrated is that nutrients are the primary regulators of this alarm system."

Furthermore, Betley's team determined that giving mice a combination of hormones normally released during digestion significantly calmed the activity of these neurons, pointing to a potential therapeutic strategy for reducing overeating and obesity.

Betley collaborated with postdoctoral researchers and co-first authors Zhenwei Su and Amber L. Alhadeff on the study, which appears this week in the journal Cell Reports.

The brain's hypothalamus, which controls the sensation of hunger, is home to a population of neurons known as agouti-related protein-expressing neurons, or AgRP neurons. In earlier work, Betley and colleagues discovered that these neurons are highly active when an animal is hungry, that their activity is suppressed upon eating and that merely smelling or seeing food can also lead to a rapid decline in their activity. All signs pointed to these neurons functioning as a way for to avoid starvation.

"Being hungry can feel unpleasant, and these are the neurons that seem to mediate this," Betley said. "Animals don't like this stimulation. In the laboratory, we can turn these neurons on with the flash of a light. Interestingly, we found that animals will scamper to the other side of the room to turn off the light."

In the current study, the researchers wanted to distinguish between the effects of simply seeing or smelling food and actually consuming food on reducing the activity of the AgRP neurons.

They worked with mice that had been genetically engineered so that calcium, a marker of neuronal activity, increased the fluorescence of a molecular reporter in their AgRP neurons, allowing neuronal activity to be easily tracked as the animals were awake and moving about. Like the researchers had seen before, when they presented animals with normal chow, AgRP neuron activity rapidly decreased and stayed low during and after eating.

But what if the mice were presented with a food they'd never seen before and thus didn't associate with satiety? When they gave hungry mice a strawberry-flavored, calorie-free gel, a food item with which the animals were unfamiliar, the sight and smell had no effect on AgRP neuron activity levels. Ingesting the calorie-free gel led to a transient decrease in AgRP neuron activity, but this lasted only around 200 seconds. Repeated trials with the calorie-free gel led to a decrease in this transient effect showing that the animal comes to associate the taste and flavor characteristics of this food with a lack of calories.

But when those same animals were later given a calorie-containing gel with the same flavor profile, AgRP neuron activity declined after eating and stayed low. And upon a second exposure to the caloric gel, the researchers saw the mice experienced an anticipatory drop in AgRP neuron activity, suggesting that they had learned to associate the flavor and sight of the gel with satiety.

The researchers then reversed the experiment with a different group of mice, first giving the animals a calorie-containing gel. Again they saw no anticipatory drop in AgRP neuron activity in the first trial, but there was a sustained reduction in neuron activity after eating. When the researchers then gave these mice the calorie-free gel, they observed the anticipatory drop in AgRP neurons, but this lasted only 200 seconds and was likely due to the animals anticipating the consumption of calories. After 200 seconds, the neurons began firing again after the stomach "realized" it hadn't acquired any nutrients.

"In a single trial, the circuits in the brain's sensory system learn to associate the visual or olfactory stimulus of the [gel] with calories," Betley said. "If they get the caloric kind first and then see the calorie-free kind, they predict the nutrients because the flavor is the same, the taste is similar. It takes 200 seconds for the animal to realize that whatever has hit its gut isn't doing what it should be doing and the activity of those neurons comes back."

To confirm that it was only the caloric food and not external sensory input that led to the suppression of AgRP neuron activity, the researchers directly infused Ensure into the animals' stomachs and found sustained and dose-dependent reductions in AgRP neuron activity. The same calorie-dependent effect was seen when they switched the infusion to pure sugars, fats or protein. In other words, the more calories in the stomach of the mice, the stronger the reduction of neuron activity.

Confident that the nutrients were delivering a signal to the brain, the researchers next wanted to ascertain how they were doing it. They found that giving mice hormones, such as cholecystokinin, peptide tyrosine tyrosine and amylin, which are normally secreted during digestion, led to dramatic and dose-dependent decreases in AgRP neuron activity. And when the team combined low doses of these three peptides, they acted synergistically, leading to a robust decrease in AgRP neuron activity.

The combinatorial approach may have relevance to treating obesity in humans, Betley noted. While therapies involving these three hormones given individually are successfully used in humans, the doses required to have an effect often lead to nausea. But the researchers saw no sign of malaise in the when they were given the cocktail of three hormones, likely because each hormone is given at low doses.

Betley's team hopes to further explore the ways in which these hormones suppress AgRP neuron activity, perhaps looking for a way to trigger their release by mimicking the presence of nutrients in the gut. Not only could the work inform pharmaceutical approaches to treating obesity, but it could lend insight into behavioral strategies that could reduce total food intake.

"It would be interesting to see whether consuming smaller meals more frequently might lead to less activity in the neurons and thus less food intake overall," Betley said. "Or maybe we can develop better combinations of foods or better ways of eating so we can avoid that 9 p.m. binge on Oreo cookies when you've had a really great diet all day."

Explore further: Heparin stimulates food intake and body weight gain in mice

More information: Cell Reports (2017). DOI: 10.1016/j.celrep.2017.11.036

Related Stories

Heparin stimulates food intake and body weight gain in mice

September 5, 2017
Heparin is a medication widely used to prevent blood clotting; it is named after and mimics the naturally occurring anticoagulant in the body. However, research published today in Cell Reports shows a novel role of heparin ...

Scientists uncover mechanism that controls feeding behavior in mice

November 9, 2016
A team of scientists has discovered a mechanism that controls feeding behavior in mice by regulating the electrical activity of a few thousand brain cells. The researchers, from Baylor College of Medicine, South China Agricultural ...

Why our brain cells may prevent us burning fat when we're dieting

May 23, 2017
A study carried out in mice may help explain why dieting can be an inefficient way to lose weight: key brain cells act as a trigger to prevent us burning calories when food is scarce.

'Hunger' neurons in the brain are regulated by protein activated during fasting

July 6, 2016
Neurons in the brain that control hunger are regulated by AMPK, a protein activated during fasting, report researchers from Tufts University School of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School ...

Hate to diet? It's how we are wired

April 27, 2015
If you're finding it difficult to stick to a weight-loss diet, scientists at the Howard Hughes Medical Institute's Janelia Research Campus say you can likely blame hunger-sensitive cells in your brain known as AGRP neurons. ...

Fat tissue in energy saving mode

March 31, 2016
Scientists at the Max Planck Institute for Metabolism Research in Cologne have discovered brain cells that not only tell hungry mice to search for food, but also to limit blood sugar use by the brown fat tissue. This could ...

Recommended for you

Regulating microglial activity may reduce inflammation in neurodegenerative diseases

October 17, 2018
A group of Massachusetts General Hospital (MGH) investigators is proposing that targeting immune checkpoints—molecules that regulate the activity of the immune system—in immune cells called microglia could reduce the ...

New imaging tool captures how sound moves through the chinchilla ear

October 17, 2018
Researchers have developed a new device that can be used to visualize how sound-induced vibrations travel through the ear. The technology is providing new insight into how the ear receives and processes sound waves and, with ...

Sensory perception is not a one-way street

October 17, 2018
When we interact with the world, such as when we reach out to touch an object, the brain actively changes incoming sensory signals based on anticipation. This so-called 'sensory gating' has now been investigated by neuroscientists ...

Environmental factors may trigger onset of multiple sclerosis

October 16, 2018
A new Tel Aviv University study finds that certain environmental conditions may precipitate structural changes that take place in myelin sheaths in the onset of multiple sclerosis (MS). Myelin sheaths are the "insulating ...

Study points to possible new therapy for hearing loss

October 15, 2018
Researchers have taken an important step toward what may become a new approach to restore hearing loss. In a new study, out today in the European Journal of Neuroscience, scientists have been able to regrow the sensory hair ...

Scientists examine how neuropathic pain responds to Metformin

October 15, 2018
Scientists seeking an effective treatment for one type of chronic pain believe a ubiquitous, generic diabetes medication might solve both the discomfort and the mental deficits that go with the pain.


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.