Therapeutic antibodies protected nerve–muscle connections in a mouse model of Lou Gehrig's disease

Amyotrophic Lateral Sclerosis
An MRI with increased signal in the posterior part of the internal capsule which can be tracked to the motor cortex consistent with the diagnosis of ALS. Credit: Frank Gaillard/Wikipedia

Amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig's disease, causes lethal respiratory paralysis within several years of diagnosis. There are no effective treatments to slow or halt this devastating disease. Mouse models of ALS reproduce the hallmarks of the disease, including a loss of nerve-muscle connections, called neuromuscular synapses, and a subsequent loss of nerve cells that connect to muscle, called motor neurons.

Now a new study led by NYU School of Medicine researchers identifies a novel treatment strategy that preserved neuromuscular in a mouse model of aggressive ALS. Published February 20 in the journal eLife, the study found that the loss of such synapses was reduced in ALS mice injected with that stimulate a molecule in muscle which keeps nerves attached to the muscle.

By boosting the action of a protein called MuSK, the new approach slowed the loss of neuromuscular synapses, temporarily extended survival of , and extended the lifespan of ALS mice.

"Our findings reveal a new therapeutic strategy for ALS that protects a pathway essential for keeping nerves and muscles connected," says Steven Burden, PhD, a professor in the Skirball Institute of Biomolecular Medicine, and in the Department of Neuroscience and Physiology, at NYU School of Medicine.

"There are few treatments for ALS, and the two FDA-approved drugs extend survival for only a few months in a subset of patients," says Burden. "We believe that our approach, mostly likely in combination with other drugs, may extend quality of life during the early phases of this ."

Study Details

The results are based on the ability of neurons to pass messages to one another or to other targets, including skeletal muscle, at specialized junctions. Motor neurons connect to skeletal muscle at neuromuscular synapses, and the dismantling of these synapses is an early sign of disease in ALS. Although this dismantling is known to be a primary cause of ALS paralysis, most experimental treatments have sought to instead stop the death of motor neurons, which happens later in the disease course, researchers say.

The new approach centers on MuSK, a receptor tyrosine kinase on the muscle cell surface. Once stimulated by a signal provided by the , MuSK provides instructions for building the neuromuscular synapse, including the attachment of motor nerve endings to muscle.

To test whether increasing attachment between nerve and muscle might keep synapses connected when they would otherwise be dismantled in ALS mice, the researchers used an antibody that stimulates MuSK to boost signaling from muscle to nerve. A single dose of a MuSK-stimulatory antibody, introduced into ALS mice after disease onset, increased the number of fully innervated neuromuscular synapses 2.6-fold.

Chronic dosing with the stimulatory antibody led to a sustained increase in the number of neuromuscular synapses for two months, and improved function of diaphragm , which is critical for breathing. Untreated ALS mice survive for about five months, and the MuSK-stimulatory antibody prolonged their survival by about week.

"The therapeutic strategy described here targets a disease mechanism, namely the loss of neuromuscular synapses, which is common to familial and sporadic forms of ALS; and is based on a therapeutic antibody format with considerable clinical precedence," says Burden. "Although the MuSK agonist antibody cannot override the many pathological pathways that occur in motor neurons and in non-neuronal cells, therapeutic interventions that preserve neuromuscular synapses have the potential to improve the quality of life for a majority of ALS patients."

More information: Sarah Cantor et al. Preserving neuromuscular synapses in ALS by stimulating MuSK with a therapeutic agonist antibody, eLife (2018). DOI: 10.7554/eLife.34375

Journal information: eLife

Provided by NYU Langone Health
Citation: Therapeutic antibodies protected nerve–muscle connections in a mouse model of Lou Gehrig's disease (2018, February 20) retrieved 1 October 2023 from
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