E-cigarettes can damage DNA

August 20, 2018, American Chemical Society
Credit: CC0 Public Domain

The popularity of electronic cigarettes continues to grow worldwide, as many people view them as a safer alternative to smoking. But the long-term effects of e-cigarette usage, commonly called "vaping," are unknown. Today, researchers report that vaping may modify the genetic material, or DNA, in the oral cells of users, which could increase their cancer risk.

The researchers will present their results today at the 256th National Meeting & Exposition of the American Chemical Society (ACS).

"E-cigarettes are a popular trend, but the long-term health effects are unknown," says Romel Dator, Ph.D., who is presenting the work at the meeting. "We want to characterize the chemicals that vapers are exposed to, as well as any DNA damage they may cause."

Introduced to the market in 2004, e-cigarettes are handheld electronic devices that heat a liquid, usually containing nicotine, into an aerosol that the user inhales. Different flavors of liquids are available, including many that appeal to youth, such as fruit, chocolate and candy. According to a 2016 report by the U.S. Surgeon General, 13.5 percent of , 37.7 percent of and 35.8 percent of young adults (18 to 24 years of age) have used e-cigarettes, compared with 16.4 percent of older adults (25 years and up).

"It's clear that more carcinogens arise from the combustion of tobacco in regular cigarettes than from the vapor of e-cigarettes," says Silvia Balbo, Ph.D., the project's lead investigator, who is at the Masonic Cancer Center at the University of Minnesota. "However, we don't really know the impact of inhaling the combination of compounds produced by this device. Just because the threats are different doesn't mean that e-cigarettes are completely safe."

To characterize chemical exposures during , the researchers recruited five users. They collected saliva samples before and after a 15-minute vaping session and analyzed the samples for chemicals that are known to damage DNA. To evaluate possible of vaping, the team assessed DNA damage in the cells of the volunteers' mouths. The researchers used mass-spectrometry-based methods they had developed previously for a different study in which they evaluated oral DNA damage caused by alcohol consumption.

Dator and Balbo identified three DNA-damaging compounds, formaldehyde, acrolein and methylglyoxal, whose levels increased in the saliva after vaping. Compared with people who don't vape, four of the five e-cigarette users showed increased DNA damage related to acrolein exposure. The type of damage, called a DNA adduct, occurs when toxic chemicals, such as acrolein, react with DNA. If the cell does not repair the damage so that normal DNA replication can take place, cancer could result.

The researchers plan to follow up this preliminary study with a larger one involving more e-cigarette users and controls. They also want to see how the level of DNA adducts differs between e-cigarette users and regular cigarette smokers. "Comparing e-cigarettes and tobacco cigarettes is really like comparing apples and oranges. The exposures are completely different," Balbo says. "We still don't know exactly what these e-cigarette devices are doing and what kinds of effects they may have on health, but our findings suggest that a closer look is warranted."

Explore further: E-cigarette vapor disables key immune cells in the lung and boosts inflammation

More information: Integrating multi-"omics"- mass spectrometry-based methods to characterize electronic cigarette exposure in humans, the 256th National Meeting & Exposition of the American Chemical Society (ACS).

Abstract
Characterizing chemical exposures in humans remains a significant challenge. Our lab has been developing state-of-the-art high-resolution mass spectrometry-based methods to characterize the exposome and determine how it may influence overall health. Because it is challenging to capture the complexity of exposures, often at trace levels, improved tools are needed to move this field of research forward. We have recently developed a neutral loss (NL) screening and relative quantitation strategy for the targeted analysis of reactive carbonyls in biological fluids. In addition, a comprehensive DNA adductomics approach was developed to characterize covalent modifications in DNA generated from these exposures. Here, we have integrated these MS-based methods to characterize electronic cigarette exposure in humans, with the goal of identifying reactive carbonyls generated during vaping and the corresponding DNA adducts formed in the oral cavity. Human saliva and oral cell samples from e-cigarette users (n=10) and non-user controls (n=10) were obtained to screen reactive carbonyls and DNA adducts. Reactive carbonyls in saliva were derivatized with 2,4-dinitrophenylhydrazine to form hydrazones and analyzed by the NL screening method. Likewise, DNA from oral cells were isolated, hydrolyzed to nucleosides, and analyzed by both targeted and non-targeted DNA adductomics approaches. Using the NL loss screening strategy, increased levels of acrolein, methylglyoxal, and formaldehyde were observed after vaping, while the levels of acetaldehyde and glyoxal vary within subjects possibly due to variations in composition of the e-liquid used. This information was then used to develop targeted and non-targeted DNA adductomics approaches to monitor the corresponding DNA adducts in oral cells of e-cigarette users. Our results showed increased levels of acrolein-derived DNA adducts, in particular, gamma-OH-Acro-dG in e-cigarette users compared to non-users. We are currently investigating other DNA adducts that might be relevant to e-cigarette exposure using non-targeted DNA adductomics approaches and expanding our analysis to a larger sample size.

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11 comments

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Theseus
5 / 5 (2) Aug 20, 2018
Formeldahyde? Really, big tobacco? I had heard that the big e-cig companies put stuff like that in their products, but I'd hoped it was a myth. There's no absolutely no reason to include an ingredient like that other than to kill people.

I make my own liquid, and I use only the two essential ingredients that all e-liquid use: vegetable glycerine and nicotine. No flavors or any BS sweeteners. Vaping keeps me from reverting back to smoking cigarettes.

I'd love to see a study done on the most basic ingredients, including propylene glycol, which I don't use anymore. Then we'd have a much better understanding about vaping.

Seeing as how there's a plethora of e-liquids on the market with varying flavors, sweeteners, and straight up unnecessary additives, any study that doesn't take the basic ingredients into account is almost worthless to someone who vapes a different product than the one used in the study.
johnhew
5 / 5 (2) Aug 20, 2018
Didn't see a publication yet but not clear how/where the formaldehyde, acrolein and methylglyoxal that is being measured here is actually formed; not necessarily 'added'.
chemhaznet1
3 / 5 (2) Aug 20, 2018
Ah and of course "The researchers will present their results today at the 256th National Meeting & Exposition of the American Chemical Society (ACS)" but in the meantime lets take a crap all over e-cigarettes for big tobacco. It would be nice to have noted who helped fund the study in this article at the very least.
Old_C_Code
1 / 5 (1) Aug 20, 2018
"...all over e-cigarettes for big tobacco"
Big tobacco makes the vape products used at my local store.
carbon_unit
1 / 5 (1) Aug 20, 2018
No, Theseus, they aren't adding stuff like formaldehyde to kill people (or allowing a situation where it can be formed), they are adding stuff to make a bunch of money!! Experience with the tobacco industry shows they were adding all kinds of stuff to enhance the flavor/addictive power of cigarettes. Killing consumers was just an unfortunate side effect. Why should big nicotine behave any differently as it creates a new generation of fools who allow themselves to become addicts, believing it is safe?
Edit: Your uses of vaping, to avoid smoking, is the only legitimate use of e-cigarettes, IMHO.
Alan_J_Perrick
1 / 5 (1) Aug 20, 2018
"Anti-racists" say there's a RACE problem. They say it'll be solved when non-Whites pour into ALL & ONLY White nations and "assimilate" to get a brown mixture.

They say only White nations have this RACE problem; they say non-White nations are fine.

If I object to my own genocide these "anti-racists" say I am a naziwhowantstokillsixmillionjews.

They say they're anti-racist. What they are is anti-White.

Anti-racist is a codeword for anti-White.
ReedThompson
not rated yet Aug 20, 2018
This study bought and paid for the Tobacco Growers Association of 'Murica.
NJ citizen
5 / 5 (1) Aug 20, 2018
Now perform a study, modeled identically, against consumption of presumably innocuous food such as

• lettuce or apples

or somewhat more conspicuous fare, say

• barbecue or other charred meats
• anything smoked
• or hot, spicy dishes that burn the mouth

Do I sense an agenda here?
Anonym414679
not rated yet Aug 20, 2018
... May modify DNA? This statement is a guess. Or it may not. So there, so much for expert opinion. What nonsense!
JuanitaBroaddricksUpperLip
5 / 5 (1) Aug 20, 2018
Follow the money trail. Inhaling commercially-produced chemicals...what could possibly go wrong?
Anonym518498
not rated yet Aug 20, 2018
I bet you weed vaping is good though 'cause weed cures everything

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