Deregulated mTOR is responsible for autophagy defects, exacerbating kidney stone formation

Deregulated mTOR is responsible for autophagy defects exacerbating kidney stone formation
Numbers of GFP-MAP1LC3 puncta (white arrows) and autophagosomes (black arrows) in renal tubular cells were increased with mTOR inhibitor of GFP-MAP1LC3-mice treated with GOX. This means the activation of autophagy. Amount of crystals formed in kidneys extracted from mice after GOX and mTOR inhibitor injection was suppressed. Credit: Takahito Yasui, M.D., Ph.D.

Kidney stone disease is a lifestyle-related disease; however, effective medical treatment is not yet well established. As cellular damage in renal tubular cells is responsible for the disease, researchers have focused on the role of autophagy in a new study. They report that autophagy compromised by mTOR deregulation is a fundamental feature in the pathology of kidney stone formation, and propose that chemical inhibition of mTOR could be a prospective strategy for disease suppression.

Dr. Takahiro Yasui (Professor, Nagoya City University) and Dr. Rei Unno (Research fellow, Nagoya City University) in collaboration with Dr. Tsuyoshi Kawabata (Associate Professor, Nagasaki University) have revealed a novel mechanism for stone formation using a for kidney stone disease, and human kidney tissue. They found that autophagic activity was significantly decreased in mouse renal tubular cells (RTCs) exposed to (CaOx) monohydrate crystals and in the kidneys of GFP-conjugated MAP1LC3B (microtubule- associated protein 1 light chain 3 beta) transgenic mice with CaOx nephrocalcinosis induced by glyoxylate (GOX). This caused accumulation of damaged intracellular organelles, such as mitochondria and lysosomes, the normal functioning of which is mediated by functional .

An impairment of autophagy was also observed in the mucosa with plaques of CaOx kidney stone formers. Moreover, they determined that the decrease in autophagy was caused by an upregulation of mTOR, which consequently resulted in the suppression of the upstream autophagy regulator TFEB (transcription factor EB). Furthermore, they showed that an mTOR inhibitor could recover a decrease in autophagy and alleviate crystal-cell interactions and the formation of crystals associated with increased inflammatory responses (Figure 1). As chemical inhibition of mTOR ameliorates kidney stone development, this result proposed that deregulated mTOR and resultant impairment in autophagy is a key target for prevention or treatment of the (Figure 2).

Deregulated mTOR is responsible for autophagy defects exacerbating kidney stone formation
During kidney stone development, mTOR is activated and nuclear TFEB expression is suppressed, impairing autophagy. Ablation of autophagy causes accumulation of damaged organelles. This enhances the inflammatory response, which facilitates crystal-cell interactions and promotes kidney stone formation. Therefore, autophagy defect caused by deregulation of the mTOR-TFEB axis could be an effective target for kidney stone formation. An mTOR inhibitor, TFEB inducer, or autophagy inducer could suppress the development of kidney stones. Credit: Takahito Yasui, M.D., Ph.D.

More information: Rei Unno et al, Deregulated MTOR (mechanistic target of rapamycin kinase) is responsible for autophagy defects exacerbating kidney stone development, Autophagy (2019). DOI: 10.1080/15548627.2019.1635382

Provided by Nagoya City University
Citation: Deregulated mTOR is responsible for autophagy defects, exacerbating kidney stone formation (2019, August 7) retrieved 15 July 2024 from
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