Research finds potential target for tumor-causing condition

UC research finds potential target for tumor-causing condition
Chenran Wang, assistant professor in the UC Department of Cancer Biology, and Jun-Lin Guan, Francis Brunning Endowed Chair and professor of cancer biology at UC. Credit: Colleen Kelley / University of Cincinnati

University of Cincinnati researchers have found additional ways to target the molecular processes involved in activating a certain protein complex to potentially develop new therapies for a tumor-causing disorder.

These findings are being published in the Nov. 11 issue of the journal Nature Metabolism.

"Tuberous sclerosis complex, or TSC, is a genetic disorder that causes tumors to form in many different organs including the brain and affects as many as 50,000 people in the U.S. yearly," says Dr. Chenran Wang, co-corresponding and leading author of the paper and assistant professor in the UC Department of Cancer Biology. "TSC can also affect the brain by causing seizures and autism, among other issues, in newborns and adults. In this condition, the mutations in the genes called Tsc1 or Tsc2 lead to loss of their tumor suppressing functions, which in turn, results in the hyper activation of mTORC1 and its abnormal function, responsible for the multiple symptoms of TSC."

Known as a "master regulator" of , mTORC1 is involved in causing the activity of most cell growth; however, it does not promote —a regulation method when a cell basically eats itself and an efficient way for fuel generation inside cells.

In this research, scientists saw higher autophagy activity in cells without Tsc1; they then created a "double knockout" —a model without both Tsc1 and an essential autophagy protein, FIP200, present in the developing nervous system and in adult neural stem cells.

"Using this unique model, we uncovered the essential functions of autophagy as a way to keep mTORC1 activity high and to show the abnormal development of neural stem cells without Tsc1," Wang adds.

Researchers looked at the molecular and metabolic mechanisms of autophagy involved in maintaining high mTORC1 activity and found that it was needed to activate the energy storage, namely lipid droplets in the cells without Tsc.

"The breakdown of lipid droplets by autophagy supplied fatty acid as an energy source to sustain energy production in Tsc1-deficient neural stem cells," Wang says. "We also used pharmacological methods to target autophagy and block used to treat defects, mimicking human TSC symptoms in these models."

Co-corresponding author on the study Jun-Lin Guan says these results are key in expanding knowledge of the formation of this disease and how it can be targeted at the .

"We are now more informed about the signaling pathways and metabolic alterations involved in TSC caused by Tsc gene mutations and overactive mTORC1," says the Francis Brunning Endowed Chair and professor of cancer biology at UC. "This will help in the development of new therapeutic concepts for treatment of patients with this devastating disease."


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Two independent mechanisms are involved in tuberous sclerosis

More information: Autophagic lipid metabolism sustains mTORC1 activity in TSC-deficient neural stem cells, Nature Metabolism (2019). DOI: 10.1038/s42255-019-0137-5 , https://nature.com/articles/s42255-019-0137-5
Journal information: Nature Metabolism

Citation: Research finds potential target for tumor-causing condition (2019, November 11) retrieved 12 December 2019 from https://medicalxpress.com/news/2019-11-potential-tumor-causing-condition.html
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