Brain organoids with the potential to predict drug permeability

Brain organoids with the potential to predict drug permeability
Microscopy image of the highly convoluted choroid plexus epithelium in organoids expressing tight junction markers and membrane transporters that regulate entry of molecules into the brain. Credit: MRC Laboratory of Molecular Biology

The human brain is bathed in a supportive fluid called the cerebrospinal fluid (CSF) that provides nutrients and is required for proper brain function. The composition of human CSF and how it is made are poorly understood due to a lack of experimental access. Madeline Lancaster's group in the LMB's Cell Biology Division has now developed a new brain organoid that produces CSF and has the potential to predict whether drugs can access the brain.

CSF is produced and secreted by a tissue found deep within the called the choroid plexus (ChP). The ChP also filters blood, acting as a barrier to most substances transported in the blood, while selectively permitting access of certain small molecules. To study the development and function of the human ChP, including how CSF is made, Madeline's group developed a new of this tissue.

What are organoids?

Organoids are collections of organ-specific cell types that are produced from stem and can be studied as simplified miniature versions of organs. Organoids display similar organization of cell types to the organ they model and can perform some specific functions.

Scientists produce organoids by directing stem cells toward particular cell types through the use of signaling molecules, similarly to how the tissues would arise during development.

To develop a human-CSF-in-a-dish model, Laura Pellegrini from Lancaster's group established a protocol to produce ChP organoids from human based on the group's method for producing . These organoids display key features of human ChP and develop CSF-filled compartments that are isolated from the surrounding culture media in which the organoids are grown.

Brain organoids with the potential to predict drug permeability
Drawing of choroid plexus (ChP) organoids producing CSF-like fluid protected from the outside by a tight barrier that is highly similar to the barrier of the actual human brain. The ability of drugs, such as dopamine and L-dopa (pictured) to cross this barrier can be examined by extracting the fluid and analyzing it for their presence. Credit: MRC Laboratory of Molecular Biology

The team found that this fluid contained known CSF biomarkers and they were able to observe changes in secretion of CSF components over time, as well as the distinct cell types contributing to these dynamic changes of CSF composition. Importantly, they discovered a previously unidentified cell type in the ChP: myoepithelial cells. These cells could be important in the generation of mechanical forces involved in CSF secretion.

The ChP organoids were also found to form a tight barrier exhibiting the same selectivity for small molecules as is seen for the ChP in the brain. For example, they prevented entry of the small molecule dopamine, but allowed transport of its precursor, L-Dopa. This demonstrates the accuracy of the model with regard to the tissue it represents, which means that ChP organoids could have predictive potential for the permeability of novel drugs. To demonstrate this, the team investigated one drug that recently failed in phase 1 , BIA-10-2474, and report that the organoids could have predicted that the would inappropriately accumulate and cause neurotoxicity.

The composition of the brain barrier is different between species, so development of a human-specific ChP model is important for its capacity to predict the permeability of novel drugs and reduce the number of new drugs that fail in phase 1 clinical trials. This model is also a source of more authentic CSF that has been made specifically by ChP tissue. This will enable scientists to study the secretion of factors and disease-related biomarkers whose functions are still not well understood.

More information: Laura Pellegrini et al. Human CNS barrier-forming organoids with cerebrospinal fluid production, Science (2020). DOI: 10.1126/science.aaz5626

Journal information: Science
Provided by MRC Laboratory of Molecular Biology
Citation: Brain organoids with the potential to predict drug permeability (2020, June 12) retrieved 26 February 2024 from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Researchers create three-dimensional organoid models of human forebrain


Feedback to editors