Hydra in control media before contraction (left) and during contraction (right). Credit: Wataru Yamamoto

Although it may seem counterintuitive, researchers are turning to an animal without a brain to crack the neural code underlying behavior.

Hydra vulgaris, a tiny, tentacled, freshwater organism, uses "nets" of neurons dispersed throughout its tube-like body to coordinate stretching, contracting, somersaulting, and feeding movements. This simple nervous system is one reason that Hydra is well suited for studying how translates into motion.

In a study published in eNeuro, a duo from Columbia University and the Marine Biological Laboratory (MBL) has begun to crack the behind Hydra's simplest behavior, called contraction bursts (when the torso shrinks down and expands outward, over and over again). The scientists found that the concentration of dissolved particles in the surrounding water—a property known as osmolarity—affects the activity of a neural circuit in one of Hydra's nerve nets, which can trigger a specific set of muscle cells to contract the torso.

"One by one, we want to decipher the neural and muscular activity behind each of Hydra's behaviors," says senior author and MBL Whitman Center Fellow, Rafael Yuste of Columbia University. "This paper is the beginning of our journey."

Five Hydra freely behaving in 50mM sucrose (high osmolarity) solution. This video is sped up 300-fold. Credit: Wataru Yamamoto

Yuste and first author Wataru Yamamoto conducted their experiments in Woods Hole during the summers of 2017 and 2018, in consultation with their MBL Hydra Lab research consortium. They used whole-body calcium imaging to visualize Hydra's neurons and muscles, and tested whether tweaking various environmental conditions such as , body size, nutritional state, and osmolarity would affect contractions. They were surprised to find that just one of those parameters, osmolarity, had an impact.

Boosting the concentration of sugar particles in the water triggered fewer contractions and decreased activity in the "contraction burst" (CB) neural circuit as well as in one set of muscles. Lowering particle concentration had the opposite effect, increasing cellular activity and contractions. Although additional experiments are needed to confirm their theory, the researchers propose that CB neurons respond to changes in osmolarity by altering muscle activity, which in turn influences frequency.

Hydra ectoderm muscle activity during contraction in control media. The animal was allowed to move between coverslips in the mounted configuration. This video is sped up 20-fold. Credit: Wataru Yamamoto

Reacting to changes in particle concentration could mean the difference between life and death for Hydra—especially if salt is involved. As freshwater dwellers lacking advanced excretory systems, Hydra are not well-equipped to maintain the proper balance of salinity inside and outside their bodies. Water is constantly flowing in and out of their gastrovascular cavities, and too much internal salty solution causes them to balloon and literally explode. The researchers surmise that Hydra contract in response to high osmolarity to "wring" themselves out and expel excess water.

Freely-behaving Hydra vulgaris exhibiting different motor behaviors (elongation, contraction, somersaulting). Credit: Wataru Yamamoto

In addition to affecting contractions, osmolarity also influenced how often Hydra detached and repositioned its tube foot, likely preparing to move to a new location. Yamamoto plans to continue to investigate how and why this happens. He hopes his line of inquiry will eventually help decode a more complex behavior: somersaulting, when Hydra flips tentacles-over-tube foot, like a circus acrobat.

More information: Wataru Yamamoto et al, Whole-Body Imaging of Neural and Muscle Activity during Behavior in Hydra vulgaris: Effect of Osmolarity on Contraction Bursts, eneuro (2020). DOI: 10.1523/ENEURO.0539-19.2020