Vaping while pregnant found to have potential impact on prenatal brain development in zebrafish
A trio of researchers, two with the University of Calgary, the other McMaster University, has found that vaping has the potential of impacting prenatal brain development in zebrafish. In their paper published in the journal Biology Letters, Patrick Gauthier, Alison Holloway and Mathilakath Vijayan describe experiments they conducted with vaping and zebrafish and what they learned about the impact of vaping chemicals on maturing zebrafish.
Vaping is the act of inhaling vapor from an electronic cigarette or other similar type of device. During its initial introduction to consumers, many people believed that vaping was harmless. For that reason, many non-smokers began using e-cigarettes and many smokers switched, believing they were less harmful than regular cigarettes. Not long after their introduction, medical scientists began studying e-cigarettes and vaping to determine if they were as safe as believed. They found mixed results because of the many kinds of ingredients used to make vapor—one of which, most notably, is nicotine. In this new effort, the researchers wondered if vaping during pregnancy might have an impact on the fetus, but because they were not able to carry out experiments with humans, they used zebrafish, a very common research creature.
To test vaping in pregnant zebrafish, the researchers exposed multiple pregnant specimens to several types of vape aerosol extracts. They included those with and without added flavors, and those with and without nicotine. They allowed the eggs to be fertilized and then monitored the growing embryos using a technique called PMR to induce them to move under a light. The shells of fertilized zebrafish eggs are transparent.
The researchers found that unflavored vapes had no impact on zebrafish embryo brain development (as measured by the way they behaved just prior to hatching). Flavored vapes, on the other hand, had what the researchers describe as a profound impact on the behavior of the embryos, as did nicotine. Vapes with both flavoring and nicotine produced the most striking changes to behavior in the embryos. The researchers conclude that vaping holds the potential to adversely impact prenatal brain development in zebrafish. More research is required to determine if the same is true for humans.
More information: Patrick T. Gauthier et al. Vape flavourants dull sensory perception and cause hyperactivity in developing zebrafish embryos, Biology Letters (2020). DOI: 10.1098/rsbl.2020.0361
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