Recapitulation of the entire hepatitis C virus life in engineered mouse cell lines

A US study presented today at The International Liver CongressTM 2012 demonstrates that the entire HCV lifecycle can be recapitulated in murine cells, implying that HCV permissive mouse models could soon be developed.

The data suggests that HCV replication in the murine environment is limited by innate immune responses. Inactivating these pathways and the expression of the appropriate entry factors and miR-122 creates murine fibroblasts that can be infected and support replication.

The study also corroborates previous data that the expression of apoE promotes production of infectious virus.

Although mouse orthologs of MAVS and TRIF could be cleaved by the HCV NS3/4A protease, this was not enough to establish robust HCV replication.

In contrast, impairment of type I and type III IFN signaling enhanced the permissiveness of murine fibroblasts for persistent HCV replication.

The in-depth study of pathogenesis and immune responses is currently hampered by the lack of suitable small animal models. This study is a positive step to addressing this issue.

More information: Vogt A, Recapitulation of the entire Hepatitis C virus life in engineered mouse cell lines. Abstract presented at the International Liver Congress 2012

Provided by European Association for the Study of the Liver

not rated yet
add to favorites email to friend print save as pdf

Related Stories

Scientists Model Hepatitis C Virus

May 25, 2007

One of the most common life-threatening viral infections in the United States today is hepatitis C virus (HCV). The standard treatment is successful in only about 50 percent of treated HCV chronic patients, with no effective ...

Keeping hepatitis C virus at bay after a liver transplant

Oct 01, 2009

One of the most common reasons for needing a liver transplant is liver failure or liver cancer caused by liver cell infection with hepatitis C virus (HCV). However, in nearly all patients the new liver becomes infected with ...

Recommended for you

Study finds acupuncture does not improve chronic knee pain

4 minutes ago

Among patients older than 50 years with moderate to severe chronic knee pain, neither laser nor needle acupuncture provided greater benefit on pain or function compared to sham laser acupuncture, according to a study in the ...

Ebola outbreak nears end in Nigeria

21 minutes ago

The Ebola outbreak in Nigeria is almost over, after an ordeal that began in July when a sick man with US-Liberian citizenship flew there from Liberia, US health authorities said Tuesday.

Diuretics in proton pump inhibitor-associated hypomagnesemia

1 hour ago

Proton pump inhibitor (PPI) therapy is associated with hospitalization for hypomagnesemia, particularly among patients also receiving diuretics, according to research published this week in PLOS Medicine. The study, conduc ...

Families wait in agony for word on Ebola patients

7 hours ago

First the ring tone echoed outside the barbed-wire-topped walls of the Ebola clinic. Then came the wails of grief, as news spread that 31-year-old Rose Johnson was dead just days after she was brought here ...

User comments