Mother's stem cells likely key to treating genetic disease before birth

January 18, 2011, University of California - San Francisco
This ultrasound image shows a fetal mouse heart accompanied by an M‑mode Doppler reading of the heart rate. Credit: UCSF

( -- UCSF researchers have tackled a decade-long scientific conundrum, and their discovery is expected to lead to significant advances in using stem cells to treat genetic diseases before birth. Through a series of mouse model experiments, the research team determined that a mother's immune response prevents a fetus from accepting transplanted blood stem cells, and yet this response can be overcome simply by transplanting cells harvested from the mother herself.

"This research is really exciting because it offers us a straightforward, elegant solution that makes fetal stem cell transplantation a reachable goal," said senior author Tippi MacKenzie, MD, an assistant professor of pediatric surgery at UCSF and fetal surgeon at UCSF Benioff Children's Hospital. "We now, for the first time, have a viable strategy for treating congenital stem cell disorders before birth."

Scientists have long viewed in utero blood stem cell transplantation as a promising treatment strategy for many genetic diseases diagnosed as early as the first trimester of pregnancy, including sickle cell disease and certain immune disorders. Fetal stem cell transplantation involves taking healthy cells from the bone marrow of a donor and transplanting them into the fetus through ultrasound-guided injections. When successful, the implanted cells, or graft, replenish the patient's supply of healthy blood-forming cells.

In theory, the developing fetus with an immature immune system should be a prime target for successful transplantation, since the risk of graft rejection is low and the need for long-term immunosuppressive therapy may be avoided. However, most previous attempts to transplant blood stem cells into a human fetus have been unsuccessful, prompting some researchers to lose interest in this promising field, according to MacKenzie, who also is an investigator with the Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research.

Findings from the study will appear online January 18, 2011, in the Journal of Clinical Investigation, available at They also will be published in the journal's February 2011 issue.

"The fact that fetal stem cell transplantation has not been very successful has been puzzling, especially given the widely accepted dogma that the immature fetal immune system can adapt to tolerate foreign substances," said co-senior author Qizhi Tang, PhD, an assistant professor of transplant surgery and director of the UCSF Transplantation Research Lab. "The surprising finding in our study is that the mother's immune system is to blame."

In the study's first phase, researchers examined the cellular content of fetal mouse blood and found a large proportion of maternal blood cells in the fetus. Their analyses indicated that up to 10 percent of the fetus' blood cells came from the mother – a significantly larger percentage of maternal cells than what is found anywhere else in the fetus.

"We had previously known that a minute amount of cells travel from the mother into a developing fetus and that this is an important tolerance mechanism in all healthy pregnancies," MacKenzie said. "However, the unexpectedly large proportion of maternal blood cells in the fetus made us think that perhaps it was the maternal, rather than the fetal, immune response that poses the real barrier to effective stem cell transplantation."

To further investigate this hypothesis, the team transplanted fetal mice with blood stem cells from a second strain of mice that were not matched to the fetus or the mother. Following transplantation, the researchers observed an influx of T cells – the major driving force behind an immune response – from the mother into the fetus, which subsequently led to rejection of the transplanted graft.

However, if the researchers removed T cells only from the mother before carrying out the transplant, nearly 100 percent of the injected fetuses engrafted, or accepted the transplanted cells, indicating that maternal T cells play the critical role in triggering transplant rejection. Finally, the researchers transplanted fetal mice with blood stem cells matched to the mother, which, as expected, resulted in a very high success rate.

"As long as the transplanted stem cells are matched to the mother, it does not seem to matter if they are matched to the fetus," said first author Amar Nijagal, MD, a postdoctoral research fellow and surgery resident at UCSF. "Transplanting stem cells harvested from the mother makes sense because the mother and her developing fetus are prewired to tolerate each other."

As next steps, researchers will need to confirm that the findings are consistent in humans and also will investigate how exactly maternal T cells cause a graft rejection.

"Now that we know a fetus can become tolerant to a foreign stem cell source, we can really think big and consider looking at how other types of stem cells might be used to treat everything from neurological disorders to muscular disorders before birth," MacKenzie added.

Related Stories

Recommended for you

Researchers illustrate how muscle growth inhibitor is activated, could aid in treating ALS

January 19, 2018
Researchers at the University of Cincinnati (UC) College of Medicine are part of an international team that has identified how the inactive or latent form of GDF8, a signaling protein also known as myostatin responsible for ...

Bioengineered soft microfibers improve T-cell production

January 18, 2018
T cells play a key role in the body's immune response against pathogens. As a new class of therapeutic approaches, T cells are being harnessed to fight cancer, promising more precise, longer-lasting mitigation than traditional, ...

Weight flux alters molecular profile, study finds

January 17, 2018
The human body undergoes dramatic changes during even short periods of weight gain and loss, according to a study led by researchers at the Stanford University School of Medicine.

Secrets of longevity protein revealed in new study

January 17, 2018
Named after the Greek goddess who spun the thread of life, Klotho proteins play an important role in the regulation of longevity and metabolism. In a recent Yale-led study, researchers revealed the three-dimensional structure ...

The HLF gene protects blood stem cells by maintaining them in a resting state

January 17, 2018
The HLF gene is necessary for maintaining blood stem cells in a resting state, which is crucial for ensuring normal blood production. This has been shown by a new research study from Lund University in Sweden published in ...

Magnetically applied MicroRNAs could one day help relieve constipation

January 17, 2018
Constipation is an underestimated and debilitating medical issue related to the opioid epidemic. As a growing concern, researchers look to new tools to help patients with this side effect of opioid use and aging.


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.