Adeno-associated virus serotype-5 delivery to the rat trigeminal ganglion

March 21, 2014

Today during the 43rd Annual Meeting & Exhibition of the American Association for Dental Research, held in conjunction with the 38th Annual Meeting of the Canadian Association for Dental Research, Lauren Roper, University of Texas - San Antonio / Health Science Center, San Antonio, will present research titled "Adeno-Associated Virus Serotype-5 Delivery to the Rat Trigeminal Ganglion."

The objective of this study was to evaluate transduction efficiency of adeno-associated viruses (AAV) serotype-5 in trigeminal sensory system following a direct injection into the rat trigeminal ganglion (TG) by way of the infraorbital (IO) foramen at different survival times. Sections from TG, IO nerve and trigeminal sensory nuclei (TSN) from rats with AAV-5/CMV/chicken-beta-actin hybrid promoter with enhanced-Green Fluorescent Protein (GFP) transgene (Vector Biolabs; Philadelphia, PA) injected animals were evaluated with indirect immunofluorescence method and confocal microscopy following staining with GFP antibody.

Rats survived 2, 6 and 10 weeks (n=2/group). Viral particles delivered = 3x109 for 2 week subjects; 1.5x1010 for 6 and 10 week subjects. AAV-5 transduction, as detected by GFP staining, was present in the trigeminal system including TG sensory neurons, IO nerve peripheral processes and in central processes throughout the TSN, including the main sensory nucleus and trigeminal spinal nucleus, in 6 and 10 week survival subjects, but not in 2 week survival subjects.

Direct delivery of AAV-5 into TG can be accomplished by IO foramen injection and results in sensory neuron transduction including presence of protein (i.e. GFP) in peripheral and central processes. These results support the use of AAV-5 based gene therapy approaches to evaluate the role of target proteins and as a possible future treatment approach in trigeminal pain disorders.

Explore further: Gene therapy for lysosomal storage disease shown to be safe and well tolerated

More information: This is a summary of abstract #1280, "Adeno-Associated Virus Serotype-5 Delivery to the Rat Trigeminal Ganglion," which will be presented on Friday, March 21, 2014, 3:30 p.m. – 4:45 p.m. at the Charlotte Convention Center, Exhibit Hall AB.

Related Stories

Gamma Knife helps patients with painful facial nerve disorder

February 26, 2014

Research by Beaumont Health System radiation oncologists and neurosurgeons found that symptoms of trigeminal neuralgia, or TN, a nerve disorder causing severe facial pain, were reduced in those treated with Gamma Knife stereotactic ...

Recommended for you

Success in the 3-D bioprinting of cartilage

April 28, 2017

A team of researchers at Sahlgrenska Academy has managed to generate cartilage tissue by printing stem cells using a 3-D-bioprinter. The fact that the stem cells survived being printed in this manner is a success in itself. ...

Mouse teeth providing new insights into tissue regeneration

April 27, 2017

Researchers hope to one day use stem cells to heal burns, patch damaged heart tissue, even grow kidneys and other transplantable organs from scratch. This dream edges closer to reality every year, but one of the enduring ...

Dentistry research ID's novel marker for left-handedness

April 27, 2017

Individuals with a slender lower face are about 25 percent more likely to be left-handed. This unexpected finding was identified in 13,536 individuals who participated in three national surveys conducted in the United States.

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.