In the ongoing hunt to find better treatments for heart disease, the top cause of death globally, new research from Stanford shows promising results using an unusual strategy: photosynthetic bacteria and light.
Researchers found that by injecting a type of bacteria into the hearts of anesthestized rats with cardiac disease, then using light to trigger photosynthesis, they were able to increase the flow of oxygen and improve heart function, according to a study published today in Science Advances.
"The beauty of it is that it's a recycling system," said Joseph Woo, MD, chair of cardiothoracic surgery at Stanford and senior author of the study. "You deliver the bacteria, they take up carbon dioxide, and with energy from the light, they form oxygen."
The genesis of this somewhat mind-boggling concept sprang from scientists searching for new ways to deliver oxygen to the heart when blood flow is restricted, Woo said. This condition, known as cardiac ischemia, is most often caused by coronary artery disease.
"We thought there is an interesting relationship in nature," he said. "In nature, humans exhale carbon dioxide and plants convert it back to oxygen. During a heart attack, the muscle is still trying to pump. There's carbon dioxide but no oxygen. We wondered if there were any way to use plant cells and put them next to heart cells to produce oxygen from the carbon dioxide."
Researchers first tried grinding up spinach and kale and combining each with heart cells in a dish, but the chloroplasts—the photosynthetic organs—of those plants weren't stable enough to survive outside of the plant cell.
"So we kept looking around," Woo said. Next, they tried photosynthetic bacteria, referred to as cyanobacteria, or blue-green algae, since it has a more rugged structure necessary for living in water. They repeated the same tests to see whether these photosynthetic bacteria had the ability to survive with heart cells in a dish.
"It was a little bit pie-in-the-sky," Woo said. "But it worked really well."
The next round of experiments involved injecting the cyanobacteria into the beating hearts of anesthetized rats with cardiac ischemia. They then compared the heart function of rats with their hearts exposed to light (for less than 20 minutes) to those who were kept in the dark.
"The group that received the bacteria plus light had more oxygen and the heart worked better," Woo said. The bacteria dissipated within 24 hours, but the improved cardiac function continued for at least four weeks, he said.
"This is still very preliminary," Woo said.
The researchers plan to investigate how to apply this concept to humans and how to deliver a light source to the human heart. They are also examining the potential of using artificial chloroplasts to eliminate the need for bacteria.
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An innovative biologic system for photon-powered myocardium in the ischemic heart Science Advances. DOI: 10.1126/sciadv.1603078