Scientists uncover a new face of a famous protein, SWI2/SNF2 ATPase

May 17, 2018 by Blair Fannin, Texas A&M University
The lack of the CHR2 gene alters plant architecture. Drs. Xiuren Zhang and Zhiye Wang, along with a team of scientists in the department of biochemistry and biophysics and Institute for Plant Genomics and Biotechnology at Texas A&M University in College Station have had their findings published online as a full article in the journal Nature. Credit: Texas A&M AgriLife Research photo

A team of Texas A&M and Texas A&M AgriLife Research scientists now have a deeper understanding of a large switch/sucrose non-fermentable (SWI/SNF) protein complex that plays a pivotal role in plant and human gene expression that causes life-threatening diseases such as cancer.

Their findings could lead to more targeted therapies and help with physiological improvements in both crops and animal agriculture. The work may provide an new idea to manipulate the functions of this protein for a better therapeutic strategy for curing human cancers.

The team has been working for years on how microRNAs are produced in the model plant Arabidopsis. MicroRNAs are tiny regulatory RNA molecules widely present in multicellular organisms. In humans, microRNAs inhibit more than 60 percent of human genes and are actively exploited as potent drugs to cure human diseases, according to the scientists.

In plants, the molecules can also control many aspects of life such as plant architecture, and responses to hostile environmental conditions. MicroRNAs are also widely engineered in agricultural crops and animals for better yield and quality.

MicroRNAs are produced in a factory inside cells from long substrates that can be hundreds or thousands of bases and also contain a distinct hairpin-structure. The factory contains a scissor-like enzyme called Dicer, and some assistants that help to fetch the long substrates. One of the assistants is known as Serrate protein, Zhang said.

"Also, the shape of the substrates is very critical for microRNA production," Zhang said. "If the shapes are changed, then the substrates do not fit the Dicer scissor and can not be cut, and microRNAs are not made."

"We are interested in finding whether there are other workers in the factory," Wang said. "We used Serrate protein as a fishing bait to catch the prey; and we caught chromatin-remodeling factor 2 (CHR2)."

CHR2 is a SWI2/SNF2 motor protein with ATPase activity, Wang said.

"It also has a nickname Braham, after a creator God in Hinduism that has four heads and multiple arms, representing brilliancy and power," he said.

Wang said CHR2 is essential for producing RNA from DNA templates because its ATPase activity breaks down ATP to generate energy.

"It loosens chromatin where DNA is packed so that RNA-producing enzymes can now access the DNA templates to make RNAs including the long substrates for microRNAs," Wang said. "This is really surprising. We asked, why does CHR2 leave its own home in the chromatin and elope with Serrate into the microRNA producing factory?"

Using the plants that lack CHR2 gene, they found that the long substrates of microRNAs are reduced.

"This totally makes sense because the chromatin cannot be opened for the RNA-producing enzymes. However, the amount of final product microRNA is even increased—this is an unexpected thing," Wang said. "That meant CHR2 must have two faces: it behaves well in its home chromatin, but is a disturber in the microRNA-producing factory, causing a malfunction."

Wang wanted to test this possibility, so he mixed CHR2 with the scissor enzyme, microRNA and other cocktails.

"Indeed, the scissor failed to cut the substrates anymore," he said.

Wang also supplied CHR2 without the motor engine ATPase to the factory. This time the scissor-like enzyme functioned just fine.

"The same thing happens if the tie between CHR2 and Serrate protein is broken," he said. "That meant, CHR2, when brought into the factory by Serrate, changes the settings inside the factory through its motor activity."

The team began to search where the disorders take place. After a series of tests, they eventually found the shapes of the microRNA substrates are changed; and the scissor enzyme cannot cut the substrates anymore.

"The results are significant because they provide an additional unknown layer of microRNA level regulation. For the first time an explanation is provided for many earlier reports showing that the level of microRNA substrates in many cases does not reflect the amount of mature microRNA", according to one reviewer for the paper.

"The study is novel and exciting. It shows that the secondary structure of microRNA substrates contains a new informational code that needs to be interpreted by CHR2 and Serrate proteins (before operation of the factory)", according to the other reviewers for the paper.

"CHR2 ATPase and its associated partners in the SWI/SNF complex are immensely implicated in numerous physiological disorders and diseases in plants and human," Zhang said.

The groundbreaking work from Zhang's lab reveals the two separate functions for CHR2 in production of microRNAs.

"The work identifies a unique gene-editing target to control microRNA amount for systematically improving agricultural traits such as plant architecture, yield, quality and response to hostile environments," Zhang said.

In human the SWI/SNF complex have been known as a potent tumor suppressor," he said. In fact, mutations in SWI/SNF components have been found to occur at a frequency of greater than 20 percent across a spectrum of human cancers.

Explore further: Study shines light on gut microbiome in colon cancer

More information: Zhiye Wang et al. SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production, Nature (2018). DOI: 10.1038/s41586-018-0135-x

Related Stories

Study shines light on gut microbiome in colon cancer

May 15, 2018
Researchers have identified a correlation between gut microbial composition and microRNA expression in human colorectal cancer, according to a recent study published in the journal mSystems. The study is the first to demonstrate ...

Scientists discover a key function of ALS-linked protein

March 8, 2018
The protein FUS, whose mutation or disruption causes many cases of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), works as a central component of one of the most important regulatory systems in cells, ...

Choreographing the microRNA-target dance

January 23, 2017
Scientists face a conundrum in their quest to understand how microRNAs regulate genes and therefore how they influence human disease at the molecular level: How do these tiny RNA molecules find their partners, called messenger ...

Recommended for you

Robots grow mini-organs from human stem cells

May 17, 2018
An automated system that uses robots has been designed to rapidly produce human mini-organs derived from stem cells. Researchers at the University of Washington School of Medicine in Seattle developed the new system.

Scientists uncover a new face of a famous protein, SWI2/SNF2 ATPase

May 17, 2018
A team of Texas A&M and Texas A&M AgriLife Research scientists now have a deeper understanding of a large switch/sucrose non-fermentable (SWI/SNF) protein complex that plays a pivotal role in plant and human gene expression ...

Antibacterial in your toothpaste may combat severe lung disease

May 17, 2018
A common antibacterial substance found in toothpaste may combat life-threatening diseases such as cystic fibrosis, or CF, when combined with an already FDA-approved drug.

Blocking two enzymes could make cancer cells mortal

May 17, 2018
EPFL scientists have identified two enzymes that protect chromosomes from oxidative damage and shortening. Blocking them might be a new anticancer strategy for stopping telomerase, the enzyme that immortalizes tumors.

Emergency contact information helps researchers branch out family tree

May 17, 2018
When you go to the doctor or hospital, one piece of information that you're always asked to provide—in addition to your name, address, and insurance information—is an emergency contact. Often, that person is a blood relative. ...

Study identifies bacterial gene responsible for producing enzyme that consumes cardiac drug

May 17, 2018
Though digoxin has been widely prescribed for decades to treat a variety of heart conditions, doctors have also long understood that, for some patients, the drug simply doesn't work.

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.