Passing the COVID test in just five minutes

Passing the COVID test in just five minutes
Figure. Operating principle of artificial intelligence nanopore for coronavirus detection. Credit: Osaka University

A team of scientists headed by SANKEN (The Institute of Scientific and Industrial Research) at Osaka University demonstrated that single virus particles passing through a nanopore could be accurately identified using machine learning. The test platform they created was so sensitive that the coronaviruses responsible for the common cold, SARS, MERS, and COVID could be distinguished from each other. This work may lead to rapid, portable, and accurate screening tests for COVID and other viral diseases.

The global coronavirus pandemic has revealed the crucial need for rapid pathogen screening. However, the current gold-standard for detecting RNA viruses—including SARS-CoV-2, the that causes COVID—is reverse transcription-polymerase chain reaction (RT-PCR) testing. While accurate, this method is relatively slow, which hinders the timely interventions required to control an outbreak.

Now, scientists led by Osaka University have developed an intelligent nanopore system that can be used for the detection of SARS-CoV-2 virus particles. Using machine-learning methods, the platform can accurately discriminate between similarly sized coronaviruses responsible for different respiratory diseases. "Our innovative technology has and can even electrically identify single virus particles," first author Professor Masateru Taniguchi says. Using this platform, the researchers were able to achieve a sensitivity of 90% and a specificity of 96% for SARS-CoV-2 detection in just five minutes using clinical saliva samples.

To fabricate the device, nanopores just 300 nanometers in diameter were bored into a silicon nitride membrane. When a virus was pulled through a nanopore by the electrophoretic force, the opening became partially blocked. This temporarily decreased the ionic flow inside the nanopore, which was detected as a change in the . The current as a function of time provided information on the volume, structure, and surface charge of the target being analyzed. However, to interpret the subtle signals, which could be as small as a few nanoamps, machine learning was needed. The team used 40 PCR-positive and 40 PCR-negative saliva samples to train the algorithm.

"We expect that this research will enable rapid point-of-care and screening tests for SARS-CoV-2 without the need for RNA extraction," Professor Masateru Taniguchi explains. "A and non-invasive method such as this is more amenable to immediate diagnosis in hospitals and screening in places where large crowds are gathered." The complete test platform consists of machine learning software on a server, a portable high-precision current measuring instrument, and cost-effective semiconducting modules. By using a method, the researchers expect that this system can be adapted for use in the detection of emerging infectious diseases in the future. The team hopes that this approach will revolutionize public health and disease control.

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More information: Masateru Taniguchi et al, Combining machine learning and nanopore construction creates an artificial intelligence nanopore for coronavirus detection, Nature Communications (2021). DOI: 10.1038/s41467-021-24001-2
Journal information: Nature Communications

Provided by Osaka University
Citation: Passing the COVID test in just five minutes (2021, June 17) retrieved 28 July 2021 from
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