Cutting-edge imaging techniques for neuroscientists available in latest laboratory manual

Shown here are multicolor pyramidal neurons in the hippocampus of a Brainbow mouse, in which cells express different fluorescent proteins in a combinatorial fashion. Credit: © 2011 Cold Spring Harbor Laboratory Press

Neuroscientists have long pioneered the use of new visualization techniques. Imaging in Neuroscience: A Laboratory Manual continues that tradition by presenting an outstanding collection of methods for visualizing the nervous system. It is the third volume in a series of imaging manuals published by Cold Spring Harbor Laboratory Press.

"This series of manuals originated in the Cold Spring Harbor Laboratory course on Imaging Structure and Function of the Nervous System, taught continuously since 1991," notes Rafael Yuste, the Series Editor. "Since its inception, the course quickly became a "watering hole" for the imaging community and especially for neuroscientists and cellular and developmental neurobiologists, who are traditionally always open to microscopy approaches."

In recent years, the ability to directly observe neural events at various spatial and temporal scales has enormously expanded because of the development of new imaging technologies and novel functional indicators. This manual provides the foundational methods for imaging and presents the latest techniques for visualizing cells, synapses, neuromolecules, circuits, and in health and disease.

The manual is organized into eight sections that are roughly ordered according to spatial level of neural organization. Section 1 introduces molecular tools for labeling receptors and cells and for optical readout and control of neural activity. In Section 2 these tools are applied to the study of axonal propagation of excitation and synaptic transmission. Section 3 focuses on the integration of synaptic inputs in neuronal dendrites. Sections 4 and 5 provide tools for investigating functional properties of neurons and in brain slice preparations (in vitro) and in living animals (in vivo), respectively. Section 6 looks at the dynamic properties of various , an important area of neuroscience. Section 7 presents new methods for performing high-resolution imaging studies of the brain during behavior. Finally, Section 8 provides examples of imaging studies in various animal models of disease.

More information: This new manual is the third volume in the imaging series that began with Imaging: A Laboratory Manual(www.cshlpress.com/link/imagingp.htm), released last November, followed by Imaging in Developmental Biology: A Laboratory Manual(www.cshlpress.com/link/imagingdevbiop.htm), released in January 2011.

add to favorites email to friend print save as pdf

Related Stories

Identification of a novel neural stem cell type

Jan 14, 2008

As published in the upcoming issue of G&D, sesearchers from the Sloan-Kettering Institute, led by Dr. Lorenz Studer, have discovered a novel type of neural stem cell, which has a broader differentiation potential than previously ...

Recommended for you

New research supporting stroke rehabilitation

4 hours ago

Using world-leading research methods, the team of Dr David Wright and Prof Paul Holmes, working with Dr Jacqueline Williams from the Victoria University in Melbourne, studied activity in an area of the brain ...

Team finds an off switch for pain

9 hours ago

In research published in the medical journal Brain, Saint Louis University researcher Daniela Salvemini, Ph.D. and colleagues within SLU, the National Institutes of Health (NIH) and other academic institutions have d ...

Brain researchers pinpoint gateway to human memory

11 hours ago

An international team led by researchers of the University of Magdeburg and the DZNE has successfully determined the location, where memories are generated with a level of precision never achieved before. ...

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.