Coral reefs provide potent new anti-HIV proteins

Researchers have discovered a new class of proteins capable of blocking the HIV virus from penetrating T-cells, raising hope that the proteins could be adapted for use in gels or sexual lubricants to provide a potent barrier against HIV infection.

The proteins, called cnidarins, were found in a feathery coral collected in waters off Australia's northern coast. Researchers zeroed in on the proteins after screening thousands of natural product extracts in a biorepository maintained by the National Cancer Institute. "It's always thrilling when you find a brand-new that nobody else has ever seen before," said senior investigator Barry O'Keefe, Ph.D., deputy chief of the Molecular Targets Laboratory at the National Cancer Institute's Center for Cancer Research. "And the fact that this protein appears to block HIV infection—and to do it in a completely new way—makes this truly exciting."

In the global fight against AIDS, there is a pressing need for anti-HIV microbicides that women can apply to block HIV infection without relying on a man's willingness to use a condom. Koreen Ramessar, Ph.D., a postdoctoral research fellow at the National Cancer Institute and a member of the research team, said cnidarins could be ideally suited for use in such a product because the proteins block HIV transmission without encouraging the virus to become resistant to other HIV drugs.

"When developing new drugs, we're always concerned about the possibility of undermining existing successful treatments by encouraging drug resistance in the virus," said O'Keefe. "But even if the virus became resistant to these proteins, it would likely still be sensitive to all of the therapeutic options that are currently available."

The research team identified and purified the cnidarin proteins, then tested their activity against laboratory strains of HIV. The proteins proved astonishingly potent, capable of blocking HIV at concentrations of a billionth of a gram by preventing the first step in HIV transmission, in which the virus must enter a type of immune cell known as the T-cell.

"We found that cnidarins bind to the virus and prevent it from fusing with the T-cell membrane," said Ramessar. "This is completely different from what we've seen with other proteins, so we think the cnidarin proteins have a unique mechanism of action."

The next step is to refine methods for generating cnidarins in larger quantities so the proteins can be tested further to identify potential side effects or activity against other viruses. "Making more of it is a big key," said O'Keefe. "You can't strip the Earth of this coral trying to harvest this protein, so our focus now is on finding ways to produce more of it so we can proceed with preclinical testing."

The scientists discovered cnidarins while screening for proteins, a largely understudied component of natural product extracts found in the National Cancer Institute's extract repository. The institute maintains a large collection of natural specimens gathered from around the world under agreements with their countries of origin. The specimens are available to researchers across the United States.

"The natural products extract repository is a national treasure," said O'Keefe. "You never know what you might find. Hopefully, discoveries like this will encourage more investigators to use this resource to identify extracts with activity against infectious disease."

More information: Koreen Ramessar will present the team's findings during the Experimental Biology 2014 meeting on Tuesday, April 29 from 12:15 – 1:45 p.m. at the Mode of Action Natural Products poster session in Exhibit Halls A-D (Poster #D208), San Diego Convention Center.

add to favorites email to friend print save as pdf

Related Stories

Geranium extracts inhibit HIV-1

Jan 30, 2014

(Medical Xpress)—Extracts of the geranium plant Pelargonium sidoides inactivate human immunodeficiency virus type 1 (HIV-1) and prevent the virus from invading human cells. In the current issue of PLOS ON ...

New target to fight HIV infection identified

Oct 01, 2013

A mutant of an immune cell protein called ADAP (adhesion and degranulation-promoting adaptor protein) is able to block infection by HIV-1 (human immunodeficiency virus 1), new University of Cambridge research reveals. The ...

Recommended for you

Condoms 'too small' for Uganda men

Sep 19, 2014

Ugandan MPs have been inundated with complaints that many condoms on sale in the east African nation are too small, warning the problem is a blow to the fight against AIDS.

Withdrawal from the evolutionary race

Sep 18, 2014

In some HIV sufferers, the immune system does not fight off the immune deficiency virus. Instead, the body tolerates the pathogen. A research team headed by ETH Zurich has now determined how strongly patients ...

The genetics of coping with HIV

Sep 16, 2014

We respond to infections in two fundamental ways. One, which has been the subject of intensive research over the years, is "resistance," where the body attacks the invading pathogen and reduces its numbers. Another, which ...

User comments