News tagged with genotyping
Variation in the gene MUC5B among patients with idiopathic pulmonary fibrosis was associated with improved survival, according to a study published online by JAMA. The study is being released early online to coincide with i ...
Diseases, Conditions, Syndromes May 21, 2013 | not rated yet | 0
(HealthDay)—Older individuals with nonmelanoma skin cancer (NMSC) seem to have a significantly reduced risk of developing Alzheimer's disease (AD), according to a study published online May 15 in Neurology.
Alzheimer's disease & dementia May 16, 2013 | 5 / 5 (1) | 0
(Medical Xpress)—A new study out today reveals that the emergence and spread of a rapidly evolving invasive intestinal disease, that has a significant mortality rate (up to 45%) in infected people in sub-Saharan ...
Genetics Sep 30, 2012 | 5 / 5 (3) | 1 |
Large-scale genetic study defines relationship between primary sclerosing cholangitis and other autoimmune diseases
For the first time, scientists show that a leading cause of liver transplant, primary sclerosing cholangitis (PSC), is a distinct disease from inflammatory bowel disease, opening up new avenues for specific PSC treatments.
Genetics Apr 21, 2013 | not rated yet | 0 |
Researchers have identified 38 new genetic regions that are associated with glucose and insulin levels in the blood. This brings the total number of genetic regions associated with glucose and insulin levels to 53, over half ...
Genetics Aug 12, 2012 | 5 / 5 (1) | 0 |
With the number of doses and cost of human papillomavirus (HPV) vaccines a barrier to global implementation, researchers have found that girls who received two doses of HPV vaccine had immune responses to HPV-16 and HPV-18 ...
Cancer Apr 30, 2013 | not rated yet | 0
If you don't like the taste of pork, the reason may be that your genes cause you to smell the meat more intensely, according to a new study.
Genetics May 02, 2012 | not rated yet | 1 |
A gene so powerful it nearly triples the risk of Alzheimer's disease has been discovered by an international team including researchers from Mayo Clinic. It is the most potent genetic risk factor for Alzheimer's identified ...
Alzheimer's disease & dementia Nov 14, 2012 | 5 / 5 (1) | 0 |
Scientists have discovered that a gene that influences empathy, parental sensitivity and sociability is so powerful that even strangers observing 20 seconds of silent video identified people with a particular genetic variation ...
Genetics Nov 14, 2011 | 5 / 5 (3) | 2 |
Have you ever wondered why some people find it so much easier to stop smoking than others? New research shows that vulnerability to smoking addiction is shaped by our genes.
Genetics Sep 11, 2012 | 5 / 5 (1) | 0 |
Defying the widely held belief that a specific gene is the biggest risk factor for Alzheimer's disease, two Cornell developmental psychologists and their colleagues report that people with that gene are more ...
Alzheimer's disease & dementia Feb 12, 2013 | 5 / 5 (1) | 0 |
(Medical Xpress)—A new study has revealed how the spread of hepatitis B coincides with dates of human migration throughout history, starting around 40 000 years ago. The study could provide a framework ...
Diseases, Conditions, Syndromes Oct 11, 2012 | not rated yet | 0 |
(Medical Xpress)—Platelet disorders are heavily underdiagnosed, little understood and cannot be cured. University of Birmingham researchers and the Birmingham Platelet Group are running a UK-wide clinical trial 'Genotyping ...
Medical research Feb 11, 2013 | 5 / 5 (1) | 0 |
Lost in the euphoria of the 2003 announcement that the human genome had been sequenced was a fundamental question: how can we be sure that an individual's genome has been read correctly?
Genetics Feb 03, 2012 | 5 / 5 (3) | 0
New research shows that more than 75 per cent of people with a particular version of a gene don't produce under-arm odour but use deodorant anyway.
Health Jan 17, 2013 | 5 / 5 (2) | 6
Genotyping is the process of determining differences in the genetic make-up (genotype) of an individual by examining the individual's DNA sequence using biological assays and comparing it to another individual's sequence or a reference sequence. It reveals the alleles an individual has inherited from their parents . Traditionally genotyping is the use of DNA sequences to define biological populations by use of molecular tools. It does not usually involve defining the genes of an individual.
Current methods of genotyping include restriction fragment length polymorphism identification (RFLPI) of genomic DNA, random amplified polymorphic detection (RAPD) of genomic DNA, amplified fragment length polymorphism detection (AFLPD), polymerase chain reaction (PCR), DNA sequencing, allele specific oligonucleotide (ASO) probes, and hybridization to DNA microarrays or beads. Genotyping is important in research of genes and gene variants associated with disease. Due to current technological limitations, almost all genotyping is partial. That is, only a small fraction of an individual’s genotype is determined. New mass-sequencing technologies promise to provide whole-genome genotyping (or whole genome sequencing) in the future.
Genotyping applies to a broad range of individuals, including microorganisms. For example, viruses and bacteria can be genotyped. Genotyping in this context may help in controlling the spreading of pathogens, by tracing the origin of outbreaks. This area is often referred to as molecular epidemiology or forensic microbiology.
Humans can also be genotyped. For example, when testing fatherhood or motherhood, scientists typically only need to examine 10 or 20 genomic regions (like single-nucleotide polymorphism (SNPs)). That is a tiny fraction of the human genome, which consists of three billion or so nucleotides.
When genotyping transgenic organisms, a single genomic region may be all that needs to be examined to determine the genotype. A single PCR assay is typically enough to genotype a transgenic mouse; the mouse is the mammalian model of choice for much of medical research today.
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