Three DNA methylation markers ID recurrence in bladder cancer
Sheng-Fang Su, from USC Norris Comprehensive Cancer Center at the University of Southern California in Los Angeles, and colleagues analyzed DNA methylation levels of six markers in 368 urine sediment samples from 90 patients with noninvasive urothelial carcinoma to assess their use in longitudinal tumor recurrence surveillance. Five-fold cross-validation was used to identify the optimum marker combination, which was then validated in separate samples.
The researchers identified a panel of three markers which were able to discriminate between patients with and without recurrence. The area under the curve was 0.90 in the testing set and 0.95 in the validation set, while sensitivity was 86 and 89 percent and specificity 80 and 97 percent, respectively. Tumor recurrence was reliably predicted by the three-marker test in 80 percent of patients, which was superior to cytology and cystoscopy (35 and 15 percent, respectively). The test accurately predicted no recurrence for 74 percent of patients who scored negative on the test.
"Given their superior sensitivity and specificity in urine sediments, a combination of hyper- and hypomethylated markers may help avoid unnecessary invasive exams and reveal the importance of DNA methylation in bladder tumorigenesis," the authors write.
One author disclosed financial ties to the pharmaceutical industry.
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