Two novel cockroach allergen proteins identified

Two novel cockroach allergen proteins identified

(HealthDay)—Two novel allergen proteins from cockroaches have been identified, according to a study published online Sept. 12 in Allergy.

Yaqun Fang, from the Kunming Institute of Zoology in China, and colleagues purified and characterized two novel allergen proteins—Per a 11 (alpha-amylase) and Per a 12 (chitinase), with molecular weights around 55 and 45 kDa, respectively)—from the midgut of cockroaches. Edman degradation, , and cDNA cloning were used to determine their primary sequences.

The researchers found that sera from 39 and 30 of 47 patients (83 percent and 63.8 percent, respectively) reacted to Per a 11 and Per a 12 on immunoblots. A competitive ELISA, basophil activation test, and skin prick test further confirmed the allergenicity of Per a 11 and Per a 12.

"They appear to be of importance for the induced by cockroach, and have a potential for component-based diagnosis of allergy," the authors write.


Explore further

Immunological cross-reactions may increase food allergies

More information: Abstract
Full Text (subscription or payment may be required)
Journal information: Allergy

Copyright © 2015 HealthDay. All rights reserved.

Citation: Two novel cockroach allergen proteins identified (2015, September 21) retrieved 14 November 2019 from https://medicalxpress.com/news/2015-09-cockroach-allergen-proteins.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
82 shares

Feedback to editors

User comments