Huntington's disease affects muscle as well as neurons, study reveals

Huntington's disease affects muscle as well as neurons, study reveals
The RNA encoding mutant huntingtin (red) forms foci in the nucleus (blue) of a skeletal muscle fiber from a mouse with early-onset Huntington's disease. Credit: Miranda et al., 2017

Researchers have discovered that mice with Huntington's disease (HD) suffer defects in muscle maturation that may explain some symptoms of the disorder. The study, "Progressive Cl channel defects reveal disrupted skeletal muscle maturation in R6/2 Huntington's mice," which will be published online November 29 in The Journal of General Physiology, suggests that HD is a disease of muscle tissue as well as a neurodegenerative disorder and that therapies targeting skeletal muscle may improve patients' motor function.

HD is a progressive, and ultimately fatal, disorder caused by a mutation in the huntingtin gene that results in the production of defective huntingtin RNA and protein molecules that disrupt various cellular processes. The cognitive and psychiatric disturbances associated with HD, including memory loss and mood swings, are thought to result from the death of neurons in the striatum and cerebral cortex. But some of the disease's motor symptoms, such as involuntary movements and muscle rigidity, could arise from the effects of mutant huntingtin in skeletal muscle.

Andrew Voss and colleagues previously found that with an early-onset form of HD showed skeletal muscle defects at late stages of the disease, particularly a decrease in the function of a protein called ClC-1, which conducts chloride ions into the cell. This appeared to be caused by defective processing of the messenger RNA encoding ClC-1 and contributed to muscle hyperexcitability, potentially causing some of the motor symptoms associated with HD. But the loss of ClC-1 function could simply be a late response to the death of neurons innervating skeletal muscle; whether the chloride channel is affected during the onset and progression of HD remained unclear.

In the new study, Voss and colleagues at Wright State University in Dayton, Ohio, and California State Polytechnic University in Pomona, California, examined their HD model mice throughout the course of the disease. They found that the RNA encoding ClC-1 was misprocessed in both HD and control mice when they were young, but, as they grew older, only healthy animals were able to start correctly processing the RNA to produce functional ClC-1. Thus, even before their began to appear, ClC-1 function was reduced in the skeletal muscle of HD mice compared with healthy, control animals.

This suggested that muscle maturation might be disrupted in HD mice. Voss and colleagues found that HD mice expressed a form of the muscle motor protein myosin that is usually only produced in newborn mouse muscle. Moreover, they identified similar defects in muscle maturation in a different strain of mice with adult-onset HD.

"Our results support the idea that HD is a myopathy as well as a neurodegenerative disease and may provide a new opportunity to improve patient care by targeting ," Voss says. In addition, researchers and clinicians may be able to use the defects as biomarkers to track the progress of HD, a much easier task than examining patients' brain tissue.

More information: Miranda et al. 2017. J. Gen. Physiol.

Journal information: Journal of General Physiology

Citation: Huntington's disease affects muscle as well as neurons, study reveals (2016, November 29) retrieved 30 November 2023 from
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