For more than 100 years, the humble fruit fly has been used to understand fundamental biological processes and has been a crucial tool for rapid preclinical gene discovery for myriads of human diseases. Now, an exciting study published in eLife reports on the development of a large versatile library of fruit flies that can be used to perform efficient and elegant in vivo gene-specific manipulations using the new protocol and gene-specific integration vector CRIMIC (CRISPR-Mediated Integrated Cassette).
"We anticipate that this new collection of approximately 1,000 fly strains will significantly alter how we do fly research. These transgenic flies will accelerate the pace of biomedical discovery by serving as an easy 'one-stop shop' to answer a wide array of questions for researchers all over the world," said Dr. Hugo Bellen, professor in the Departments of Molecular and Human Genetics and Neuroscience at Baylor College of Medicine and investigator of the Howard Hughes Medical Institute in the Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital.
"In a nutshell, we have now adapted existing technology to alter thousands of flies and systematically characterize the expression and function of genes in great detail," Bellen said.
For instance, the technology should allow scientists to determine the role of a gene and the corresponding protein in any cell where the gene is expressed and whether the loss of the gene causes irreversible damage. This may be important for genes that cause human disease.
"Knowing that these flies have already helped discover many new human diseases and that they allow precise dissection of function of many variants and mutations makes the venture worthy and productive," Bellen said.
Most fly stocks are available from the Bloomington Drosophila Stock Center.
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Pei-Tseng Lee et al. A gene-specific T2A-GAL4 library for Drosophila, eLife (2018). DOI: 10.7554/eLife.35574