Researchers survey immune molecules found inside mycetoma lesions

Researchers survey immune molecules found inside mycetoma lesions
MMP-9 expression in M. mycetomatis (A), S. somaliensis (B) and A. 639 pelletieri (C). Stromal expression (i.e. connective tissue parenchymal) of MMP-9 640 was negative in all the areas which contained thick bundles of collagen fibers 641 (arrow head) (X 10). This figure is composed of representative pictures from 642 single patients. Differences among individual patients were noted, however we 643 chose pictures which correlated with the majority of each group of patients. Credit: Emanuel Siddig (CC BY 4.0)

Mycetoma is a common neglected disease caused by either fungi or bacteria which organize themselves into grains—areas of inflammation surrounded by a collagen capsule. Now, researchers reporting in PLOS Neglected Tropical Diseases have studied two immune molecules inside these grains and discovered patterns to where the molecules appear.

Mycetoma is caused by any of 56 different microorganisms and is endemic to many tropical and subtropical areas. It usually affects and children and is most common in farmers and other workers who deal directly with soil. Mycetoma appears as a slow-growing tumor-like mass that gradually increases in size. Previous research has revealed that there are three zones of inflammatory cells surrounding mycetoma grains. However, the immunopathological mechanisms involved in the chronic inflammation of mycetoma have not been well understood.

In the new work, Ahmed Hassan Fahal of the University of Khartoum, Sudan, and colleagues studied surgical biopsies from 100 patients with confirmed mycetoma. They measured of two immune proteins—interleukin-17 (IL-17) and matrix metalloprotein-9 (MMP-9)—in cells from each zone in the mycetoma grains.

While IL-17 was mostly found in Zones I and II of the , MMP-9 was present primarily in the more outer Zones II and III. Levels of the two corresponded with each other, and were associated with the disease duration and lesion size. Moreover, levels of MMP-9 also varied by the causative agent of mycetoma. In experiments in mice, animals deficient in IL-17 , granuloma formation was impaired, suggesting an importance of the molecule in early infection.

"The observations presented here are the first step and indicate that IL-17A and MMP-9 are present within the grain," the researchers say. "The functions of IL-17A and MMP-9 in the formation of the grain will be studied in ."


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More information: Siddig EE, Mohammed Edris AM, Bakhiet SM, van de Sande WWJ, Fahal AH (2019) Interleukin-17 and matrix metalloprotease-9 expression in the mycetoma granuloma. PLOS Neglected Tropical Diseases 13(7): e0007351. doi.org/10.1371/journal.pntd.0007351
Journal information: PLoS Neglected Tropical Diseases

Citation: Researchers survey immune molecules found inside mycetoma lesions (2019, July 11) retrieved 17 October 2019 from https://medicalxpress.com/news/2019-07-survey-immune-molecules-mycetoma-lesions.html
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