'A first for cancer research': New approach to study tumors
Current drugs to treat malignant tumors may be successful at reaching the tumor site but often fail to fully reach the cancerous cells in tumors.
The problem persists because tumor models used in cancer research and produced by cell culture in laboratories are not nearly the size of the actual tumors in patients. So even when a drug appears to be effective in the tiny tumors in research labs, they may perform much differently for patients.
Now, Purdue University cancer scientists are creating tumor models in 10 days that are much closer in size to the ones found in the body and readily mimic the pathological characteristics of human cancers.
"This is a first for cancer research," said Sophie Lelièvre, a professor of cancer pharmacology in Purdue's College of Veterinary Medicine. "For the first time we have created tumor models in the laboratory called macrotumors that are 0.5 to 1 centimeter in width and 1.5 centimeters in height. This is much closer to the size of small tumors detectable in patients, and remains viable for days, even weeks, enabling therapeutic drug testing."
Reaching such large tumor size in vitro was possible thanks to scaffolds prepared by Rahim Rahimi, an assistant professor of materials engineering in Purdue's College of Engineering. The scaffolds also enable peeling pieces of the large tumor for various types of analyses.
Lelièvre said that typical tumor models produced in the laboratory in three-dimensional (3-D) cell culture are between 400 and 800 micrometers. When produced in vivo, in mice, tumors in the centimeter size take weeks to grow and cannot be studied in a controlled microenvironment like in 3-D culture; and the production is costly.
"In addition to a comparable size of tumors, our in vitro models are valuable because they maintain the structure of the tumors as found in the body, and we can decide which microenvironmental characteristics of cancer to recapitulate," said Lelièvre, coleader of the Drug Discovery and Molecular Sensing Program of the Purdue Center for Cancer Research. "This is critical for testing drug delivery and finding medicines that readily target the cancerous cells in tumors and help save lives. It's another step forward in the pursuit of precision medicine."
Lelièvre said the novel tumor design was made possible because Purdue cancer researchers from across disciplines come together with support from the Purdue University Center for Cancer Research and the 3-D Cell Culture Core (3D3C) Facility of the Birck Nanotechnology Center in Purdue's Discovery Park.
"The 3D3C is really a unique facility that you won't find anywhere else in the world," said Lelièvre, who initiated 3D3C in 2015 and serves as the scientific director for the facility. "We are able to bring together engineers and biologists to create models based on 3-D cell culture, including tumor models, which help move research forward to the people who need it most."