Designing animal studies to improve research reproducibility and reduce animal use

Designing animal studies to improve research reproducibility and reduce animal use
A rack of mouse cages in an animal facilty where mice are kept under highly standardized conditions. Credit: Adrian Moser

Animal experiments are typically conducted under highly standardized laboratory conditions. While standardization is meant to improve reproducibility of scientific results, in reality reproducibility is surprisingly low. To produce more robust results, experts from different fields of research now recommend introducing biological variation into the design of animal experiments.

"The ability to reproduce through an independent replication study is the acid test by which scientists distinguish facts from mere anecdotes,"says lead author Bernhard Voelkl, who hosted the workshop together with Hanno Würbel, professor of animal welfare at the University of Bern. Würbel adds: "Poor reproducibility produces economic costs and scientific uncertainty—and also raises ethical concerns, if it hampers medical progress and animals are used for inconclusive studies."

Last year, Voelkl, Würbel and other experts in animal biology, experimental design, and biostatistics convened to discuss strategies to address this challenge. They concluded that a paradigm shift in is needed and present their considerations in the journal Nature Reviews Neuroscience.

More biological variation needed

Strict standardization of both the animals' characteristics and their environment is the norm when studying the effects of an experimental intervention, such as being given a candidate drug. Eliminating all sources of variation other than the experimental intervention is meant to increase the precision of the results, while at the same time reducing the number of animals per experiment. However, this rigorous standardization narrows down the range of animals and conditions to which the findings can reliably be generalized.

"Many animal experiments are conducted under such a narrow range of conditions that there is a significant risk of obtaining results that are unlikely to be reproducible,"says Würbel. The team therefore advocates "heterogenization", the deliberate inclusion of biological variation into the design of to improve the range of conditions to which findings can be generalized and thus improve reproducibility.

Maximizing knowledge gain per animal and experiment

"With this design we can balance the need to compare interventions under similar conditions with the ability to introduce heterogeneity, which allows us to determine whether effects are robust over a range of conditions,"says Naomi Altman, professor emeritus of statistics at Penn State. Researchers can introduce biological variation to study populations in many ways, for example, by including different strains of animals, age groups, or animals housed under different housing conditions. Alternatively, they may split experiments into several independent batches of animals or conduct multi-.

"There is no single best solution for every experiment,"says Voelkl. "Therefore, we recommend heterogenization of animals and environmental conditions in general terms. Researchers should justify their choices with respect to the range of animals and conditions to which their findings should generalize."Potential strategies should be explored in future studies to provide researchers better guidance in their choices.

The experts are convinced that by introducing biological variation into study desigsn, fewer studies, and thus fewer animals, will be needed to produce robust results. Therefore, although in some cases the number of animals used in a single study may increase, the overall number of animals used in research will be reduced, the authors emphasize. "We propose a paradigm shift to increase the benefit of the research and reduce the number of animals used in research,"says Würbel. "Instead of minimizing the number of per experiment, we should maximize the amount of knowledge we gain per animal and experiment."

To promote this , the team recommends that funders, regulators and scientific journal editors consider heterogenization as the default option and ask researchers to justify study designs in terms of the range of conditions to which their findings should apply.

More information: Bernhard Voelkl et al. Reproducibility of animal research in light of biological variation, Nature Reviews Neuroscience (2020). DOI: 10.1038/s41583-020-0313-3

Journal information: Nature Reviews Neuroscience
Provided by University of Bern
Citation: Designing animal studies to improve research reproducibility and reduce animal use (2020, June 2) retrieved 17 May 2024 from
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