Study identifies neuronal populations that drive defensive behavior in zebrafish

Study identifies neuronal populations that drive defensive behavior in zebrafish
c) Select z-plane images of volumes in an example fish showing live functional imaging (gray GCaMP signal) overlapping with the GCaMP signal after fixation and both rounds of in situ hybridization (left, pre-registration; middle, post-registration) or with in situ hybridization markers of neuropeptides from both rounds (right). Similar results were obtained for 7 fish. d) The same volumes shown in c, but containing a higher magnification of a small area. Arrowheads indicate labeled cells. eCredit: Lovett-Barron.

In vertebrates, animals that have a backbone or spinal column, many survival behaviors and physiological states are regulated by the hypothalamus. The hypothalamus is a region in the vertebrate forebrain located below the thalamus, which is known to coordinate the autonomic nervous system and play a part in the control of a number of vital body functions.

The includes different groups of transmitting chemical compounds that influence the activity of the brain and body in specific ways. These groups of cells are also known as neuropeptidergic cell populations.

While many neuroscientists have carried out studies investigating the function and structure of the hypothalamus, the ways in which different neuropeptidergic cell populations within it influence the behaviors of vertebrates is still poorly understood. Identifying the roles of these distinct groups of neurons could significantly enhance the current understanding of the hypothalamus' many functions.

With this in mind, researchers at Stanford University and University of California, San Francisco have recently carried out a study exploring the role that multiple neuronal populations in the hypothalamus play in controlling defensive responses to homeostatic threats (i.e., factors hindering an organism's ability to regulate internal conditions that promote its body's health and functioning). Their paper, published in Nature Neuroscience, specifically examines neuronal populations in the hypothalamus of zebrafish, a species of freshwater fish often used as a vertebrate model in genetics, biology and neuroscience studies.

Some past studies have found that activating different populations of neurons in the vertebrate hypothalamus can result in specific survival behaviors such as food-seeking, water-seeking, fighting, parental or defensive behaviors. While these findings highlight the roles that these groups of neurons may play in eliciting specific defensive behaviors, they are not supported by recordings of activity when neurons are in a natural state.

More recently, some research teams have tried to better understand the activity of different neuropeptidergic cell populations in the vertebrate hypothalamus by analyzing the gene expression of individual neurons after different experiences to unveil associations between specific neuron populations and the expression of activity-dependent genes. However, the results of these studies were mixed and inconsistent. In addition, gene expression analyses only allow neuroscientists to identify neurons that are strongly active during a long period of time, thus, they are far from ideal for investigating rapid defensive behaviors that take place over limited periods of time, such as avoidance or escape.

In their study, the researchers at Stanford and UC San Francisco recorded and manipulated the activity of multiple neuron populations in the neuroendocrine hypothalamus of zebrafish as they responded to a variety of homeostatic threats such as heat, acidity and movement. More specifically, they applied a technique known as cellular-resolution calcium imaging to zebrafish larvae, as in this stage of development, their hypothalamic and neuroendocrine classes are easier to access.

"Cellular registration of large-scale neural activity imaging to multiplexed in situ gene expression revealed that neuronal populations encoding behavioral features encompass multiple overlapping sets of neuropeptidergic cell classes," the researchers wrote in their paper. "Manipulations of different cell populations showed that multiple sets of peptidergic neurons play similar behavioral roles in this fast-timescale behavior through glutamate co-release and convergent output to spinal-projecting premotor neurons in the brainstem."

Overall, the researchers observed that different groups of cells in the hypothalamus expressing specific genes worked together to promote defensive behavior. In fact, when all these cell populations were surgically removed, the zebrafish no longer tried to avoid threats in their environment.

The findings of this recent study offer valuable new insight about the role that specific classes of in the hypothalamus play in enabling defensive behaviors in zebrafish and potentially other vertebrates. Combined with results collected in previous studies, the findings suggest that the hypothalamus is a highly flexible structure and that the behaviors it elicits can involve a large variety of cellular populations, as well as communication with other brain regions.

In their paper, the researchers highlight the possibility that if the hypothalamus is so flexible, commonly used "neuron type" descriptions may not adequately describe the dynamic nature of the functional neural groups contained within it. In the future, neuroscientists might thus need to introduce new ways to describe brain cell populations that better account for the interactions between neuronal groups and their multiple functions.


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Hypothalamus pathway drives defense behaviors

More information: Matthew Lovett-Barron et al. Multiple convergent hypothalamus–brainstem circuits drive defensive behavior, Nature Neuroscience (2020). DOI: 10.1038/s41593-020-0655-1
Journal information: Nature Neuroscience

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