3-D bioprinted heart provides new tool for surgeons
Professor of Biomedical Engineering Adam Feinberg and his team have created the first full-size 3-D bioprinted human heart model using their Freeform Reversible Embedding of Suspended Hydrogels (FRESH) technique. Showcased in a recent video by American Chemical Society and created from MRI data using a specially built 3-D printer, the model mimics the elasticity of cardiac tissue and sutures realistically. This milestone represents the culmination of two years of research, holding both immediate promise for surgeons and clinicians, as well as long term implications for the future of bioengineered organ research.
The FRESH technique of 3-D bioprinting was invented in Feinberg's lab to fill an unfilled demand for 3-D printed soft polymers, which lack the rigidity to stand unsupported as in a normal print. FRESH 3-D printing uses a needle to inject bioink into a bath of soft hydrogel, which supports the object as it prints. Once finished, a simple application of heat causes the hydrogel to melt away, leaving only the 3-D bioprinted object.
While Feinberg's lab has proven both the versatility and the fidelity of the FRESH technique, the major obstacle to achieving this milestone was printing a human heart at full scale. This necessitated the building of a new 3-D printer custom made to hold a gel support bath large enough to print at the desired size, as well as minor software changes to maintain the speed and fidelity of the print.
Major hospitals often have facilities for 3-D printing models of a patient's body to help surgeons educate patients and plan for the actual procedure, however these tissues and organs can only be modeled in hard plastic or rubber. Feinberg's team's heart is made from a soft natural polymer called alginate, giving it properties similar to real cardiac tissue. For surgeons, this enables the creation of models that can cut, suture, and be manipulated in ways similar to a real heart. Feinberg's immediate goal is to begin working with surgeons and clinicians to fine tune their technique and ensure it's ready for the hospital setting.
"We can now build a model that not only allows for visual planning, but allows for physical practice," says Feinberg. "The surgeon can manipulate it and have it actually respond like real tissue, so that when they get into the operating site they've got an additional layer of realistic practice in that setting."
This paper represents another important marker on the long path to bioengineering a functional human organ. Soft, biocompatible scaffolds like that created by Feinberg's group may one day provide the structure onto which cells adhere and form an organ system, placing biomedicine one step closer to the ability to repair or replace full human organs.
"While major hurdles still exist in bioprinting a full-sized functional human heart, we are proud to help establish its foundational groundwork using the FRESH platform while showing immediate applications for realistic surgical simulation," added Eman Mirdamadi, lead author on the publication.
Published in ACS Biomaterials Science and Engineering, the paper was co-authored by Feinberg's students Joshua W. Tashman, Daniel J. Shiwarski, Rachelle N. Palchesko, and former student Eman Mirdamadi.
More information: Daniel J. Shiwarski et al, Fibronectin-based nanomechanical biosensors to map 3D surface strains in live cells and tissue, Nature Communications (2020). DOI: 10.1038/s41467-020-19659-z