How changing gut microbiota can affect lupus disease activity in mice

How changing gut microbiota can affect lupus disease activity in mice
Figure 1. NZM2410 colonized with SFB exhibit intensified kidney disease with elevated immune-complex deposition. 10-week old mice were oral gavaged with fecal matter from mice harboring SFB or control mice and sacrificed at 30-weeks of age and subjected to biochemical analysis. (A) Serum blood urea nitrogen (BUN). (B) Kidney disease was directly assessed by performing hematoxylin & eosin (H&E), periodic acid-Schiff (PAS) and Jones’ silver stains. H&E, PAS and silver stain highlight enlarged glomeruli, hyaline deposits (black arrow) and subendothelial deposits (black arrowhead) in SFB colonized mice, respectively. (C) Histopathological assessment of kidney tissue was blindly scored by a veterinary pathologist; kidney pathology was defined as tubular necrosis, cellular proliferation, hyaline deposits, cellular crescents and protein casts. (D) Immunofluorescence staining of IgG and C3 deposition in the glomerular and tubulointerstitium of kidney tissue; differential interference contrast (DIC) also shown. Error bars represent mean ± SEM (A,C,D). Unpaired Student t test (A,C,D). *p < 0.05; **p < 0.005. Credit: DOI: 10.1038/s41598-021-03886-5

Research led by The Ohio State University Wexner Medical Center and College of Medicine found that introducing a single bacterium called segmented filamentous bacteria (SFB) to the gut microbiota causes detrimental effects on lupus nephritis in mice.

Study findings are published in the journal Scientific Reports.

"This is a major finding, because it provides a basis for future studies that will examine the effects of interventions that target the in the management of lupus," said lead author Dr. Wael Jarjour, a rheumatologist and director of the Division of Rheumatology and Immunology at Ohio State.

Lupus is an autoimmune disease in which the body's immune system attacks the body's own cells and organs. Lupus affects predominantly young women and can be associated with significant internal organ damage.

The Lupus Foundation of America estimates that 1.5 million Americans—and at least five million people worldwide—have a form of lupus. Men, children and teenagers also can develop this complex disease, and factors like genetics, hormones and the environment play a role in making an individual at risk for lupus.

"One of the environmental factors that plays a role is the various bacteria found in the gut, otherwise referred to as the gut . We examined the effects of a specific bacterium called segmented filamentous bacteria (SFB) in a lupus mouse model and demonstrated that the introduction of this bacterium causes severe worsening of lupus nephritis, a type of kidney disease caused by systemic lupus," Jarjour said.

Colonization of these mice with this bacterium greatly enhances inflammatory kidney lesions, he said. The normally provides a barrier similar to skin, but it becomes "leaky" once this bacterium is introduced, thus allowing some material in the intestine to leak into the circulation. SFB also alters other types of bacteria that form the microbiota in these mice, resulting in a loss of the normal balance of different bacterial species, Jarjour said.

"This bacterium has been shown to be important in rheumatoid arthritis models, and we wanted to examine its effects in lupus. Our research establishes a mouse model to test various approaches to restore gut microbiota balance," said first-author Giancarlo R. Valiente, who is a graduate of the Medical Scientist Training Program at The Ohio State University.

Researchers next plan to study if the eradication of this promotes improvement of . This would lay the groundwork for examining the effects of modifying gut microbiota in lupus patients, Jarjour said. Kidney disease caused by lupus may get worse over time and lead to kidney failure. If this happens, dialysis or a kidney transplant would be needed.

More information: Giancarlo R. Valiente et al, Gut dysbiosis is associated with acceleration of lupus nephritis, Scientific Reports (2022). DOI: 10.1038/s41598-021-03886-5

Journal information: Scientific Reports
Citation: How changing gut microbiota can affect lupus disease activity in mice (2022, January 7) retrieved 15 June 2024 from
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