Team applies fiber optics instead of lenses to open a new horizon of super-resolution photoacoustic microscopes

Exploring the world inside the body with shining fibers
A microscope system that uses optical fibers instead of lenses. Credit: POSTECH

Newly developed photoacoustic microscopy uses vibrations generated when light is absorbed and captures images of cells or blood vessels. This development has increased the possibility of peering inside the body without contrast agents.

However, obtaining super-resolution images has been limited due to the difficulty of focusing light into a small, with a lens. Recently, a POSTECH research team has opened a new horizon in the development of a super-resolution photoacoustic microscopy system by using fibers instead of lenses.

A POSTECH research team led by Professor Chulhong Kim, Dr. Byullee Park, and Ph.D. candidate Moongyu Han has successfully acquired images of photoacoustic signals using a near-field optical fiber for the first time.

When using a photoacoustic microscopy, light is usually collected into the lens at a certain distance between the light source and the sample; however, due to diffraction limitations, collecting light at a single point has been challenging.

To overcome this issue, the research team developed a microscopy system that maintains the distance between the and the sample in the near-field range (tens of nm) where the diffraction phenomenon does not occur by using a tapered fiber with a diameter of tens of nanometers.

Published in Laser & Photonics Reviews, this is the first study to apply a noncoated fiber to the photoacoustic microscopy. As the metallic component had an impact on the photoacoustic signals, conventional metallic-coated tapered fibers could not be used for photoacoustic microscopy.

As a result of the study, an evanescent wave was generated at the end of the , and the images of the sample were acquired with a resolution of 1.0 ± 0.3 micrometers. This resolution is sufficient for cellular imaging, including .

The findings from the study are an achievement that will be the cornerstone for the development of a super-resolution photoacoustic microscopy using optical tapered fibers. It can be used as a research instrument to study the fundamental life phenomena that form the basis of diseases, including and cancer.

More information: Byullee Park et al, Shear‐Force Photoacoustic Microscopy: Toward Super‐resolution Near‐Field Imaging, Laser & Photonics Reviews (2022). DOI: 10.1002/lpor.202200296

Provided by Pohang University of Science & Technology (POSTECH)
Citation: Team applies fiber optics instead of lenses to open a new horizon of super-resolution photoacoustic microscopes (2022, November 16) retrieved 20 July 2024 from
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