Sperm precursor cells made in the lab could one day restore male fertility

August 28, 2012

(Medical Xpress)—Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) can be coaxed into becoming precursor sperm cells, suggesting that it might be possible one day to restore fertility for sterile males with an easily obtained skin sample, according to researchers at the University of Pittsburgh School of Medicine. Their findings are available today in the online version of Cell Reports.

Infertility can be a side effect of some cancer treatments because the drugs work by destroying rapidly-dividing cells, which includes sperm , explained the study's lead author Charles Easley, Ph.D., formerly a post-doctoral fellow in the Department of Obstetrics, Gynecology and Reproductive Sciences at the University of Pittsburgh School of Medicine, and now a faculty member at Emory University.

"Sperm can be banked for future procedures, but that does not help some patients, such as pre-pubertal boys," Dr. Easley said. "There are procedures to store testicular tissue prior to , but men who didn't have the opportunity to save tissue are permanently sterile, and so far there are no cures for their sterility."

There is growing research evidence that adult , such as those of the skin, can be induced or biologically prodded to return to a more primitive state and then redirected to become different cell types. To see if it was possible to derive sperm cells Dr. Easley and his colleagues cultured lab-grade hiPSCs from commercially available skin samples, as well as hESCs from established cell lines, in conditions typically used to sustain spermatogonial stem cells.

They found that both kinds of stem cells were able to generate key cells, including the spermatogonial stem cells, spermatocytes containing a full complement of chromosomes prior to cell division known as meiosis, then post-meiotic spermatocytes with half the chromosome number, and round spermatids, which are precursors to sperm. Testing of certain chromosome sites showed correct parent-of-origin genomic imprints in these haploid cells as well, the researchers noted.

"No one has been able to make human sperm from in the lab, but this research indicates it might be possible," Dr. Easley said. "This model also gives us a unique opportunity to study the molecular signals that govern the process, allowing us to learn much more about how sperm are made. Perhaps one day this will lead to new ways of diagnosing and treating male infertility."

Related Stories

Recommended for you

High-fat diet starves the brain

April 29, 2016

A high-fat diet of three days in mice leads to a reduction in the amount of glucose that reaches the brain. This finding was reported by a Research Group led by Jens Brüning, Director at the Max Planck Institute for Metabolism ...

A vitamin that stops the aging process of organs

April 28, 2016

Nicotinamide riboside (NR) is pretty amazing. It has already been shown in several studies to be effective in boosting metabolism. And now a team of researchers at EPFL's Laboratory of Integrated Systems Physiology (LISP), ...

Lifestyle has a strong impact on intestinal bacteria

April 28, 2016

Everything you eat or drink affects your intestinal bacteria, and is likely to have an impact on your health. That is the finding of a large-scale study led by RUG/UMCG geneticist Cisca Wijmenga into the effect of food and ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.