Heparin might be the key to prevent prion conversion and disease

March 24, 2014

Prions are infectious agents responsible for neurodegenerative diseases such as bovine spongiform encephalitis (commonly known as "mad cow disease") and Creutzfeldt–Jakob disease in humans.

Since the discovery in the 60s that an incurable and fatal disease could be caused by an infectious agent formed by nothing but converted misfolded proteins, the mechanisms responsible for the conversion of a normal into its infectious counterpart – the scrapie prion – have been relentlessly investigated. Researchers now know that once converted into the scrapie form, these abnormal proteins have the ability to sequestrate normal proteins, which are then converted to form an increasing aggregate of fibrils that builds up mainly in the brain.

More recently, several studies have suggested that a yet unknown cofactor plays a role in the process of conversion from a normal prion into the scrapie form. Among the factors potentially involved in the process are molecules belonging to the family of glycosaminoglycans, or simply GAGs. In fact, GAGs have been implicated in several , including . However, while some studies point to these molecules as the culprit for prion conversion, others suggest an opposite effect in which the molecules protect against prion conversion.

In a previous study, a group headed by Dr. Jerson Silva at the Federal University of Rio de Janeiro in Brazil showed that when bound to , a molecule belonging to the family of GAGs, prions undergo aggregation that is similar to that observed with the scrapie form. However, this aggregation is only transient and in fact results in stabilization of the prion protein, which does not lead into prion conversion or disease.

In a paper entitled "Heparin binding confers prion stability and impairs its aggregation" and published ahead of print in The FASEB Journal, the group now unveils more details on heparin and prion conversion and presents additional evidence that might help explain the conflicting results previously reported.

Working with brain homogenates from animals with transmissible spongiform encephalopathy, the group found that heparin interactions with the terminal domains of a form of murine prion protein led to kinetic and thermodynamic stabilization of the prion protein, preventing its aggregation. "One possibility," explains Dr. Silva, "is that the negative charge of heparin molecules protects against the already known conversion effects caused by high temperatures. Alternatively, interaction of heparin with the prion protein might limit the access of the scrapie form to its normal counterpart, impairing the ability of the abnormal protein to sequestrate and convert normal prions."

Previous studies have shown that heparin of low molecular weight, as in Dr. Silva's study, is capable of crossing the blood–brain barrier, an ability essential to any drug or molecule thought to act in the brain. Additionally, LMWHep-Neuroparin, a small GAG of 2,100 Da, has shown a neuroprotective role in Alzheimer's disease animal models. Together, these findings may establish the groundwork for the development of GAG molecules for therapeutic use against prion diseases and other more common prion-like , such as Parkinson's, amyotrophic lateral sclerosis and Alzheimer's.

More information: www.fasebj.org/content/early/2014/03/18/fj.13-246777.abstract

Related Stories

New approach to protecting prion protein from altering shape

July 18, 2013

A team of researchers from Case Western Reserve University School of Medicine have identified a mechanism that can prevent the normal prion protein from changing its molecular shape into the abnormal form responsible for ...

Prion-like proteins drive several diseases of aging

September 5, 2013

Two leading neurology researchers have proposed a theory that could unify scientists' thinking about several neurodegenerative diseases and suggest therapeutic strategies to combat them.

Recommended for you

A cheaper, high-performance prosthetic knee

July 30, 2015

In the last two decades, prosthetic limb technology has grown by leaps and bounds. Today, the most advanced prostheses incorporate microprocessors that work with onboard gyroscopes, accelerometers, and hydraulics to enable ...

Crystal clear images uncover secrets of hormone receptors

July 31, 2015

Many hormones and neurotransmitters work by binding to receptors on a cell's exterior surface. This activates receptors causing them to twist, turn and spark chemical reactions inside cells. NIH scientists used atomic level ...

Flow means 'go' for proper lymph system development

July 27, 2015

The lymphatic system provides a slow flow of fluid from our organs and tissues into the bloodstream. It returns fluid and proteins that leak from blood vessels, provides passage for immune and inflammatory cells from the ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.