Evolutionary conservation of fat metabolism pathways

May 12, 2011
The metabolic system functions like a hybrid car. In the daytime we use glucose as high octane fuel, but at night we switch to the battery, which in this case is stored fat. Credit: Image: Courtesy of Dr. Marc Montminy and Jamie Simon, Salk Institute for Biological Studies.

By virtue of having survived, all animals-from flies to man-share a common expertise. All can distinguish times of plenty from famine and adjust their metabolism or behavior accordingly. Failure to do so signals either extinction or disease.

A collaborative effort by investigators at the Salk Institute for Biological Studies recently revealed just how similarly mammals and insects make critical metabolic adjustments when food availability changes, either due to environmental catastrophe or everyday changes in sleep/wake cycles. Those findings may suggest novel ways to treat metabolic conditions such as obesity and .

In a study published in the May 13, 2011, issue of Cell, co-investigators Marc Montminy, M.D., Ph.D, professor in the Clayton Foundation Laboratories for Peptide Biology, and John Thomas, Ph.D., professor in the Molecular Neurobiology Laboratory, use the fruitfly Drosophila melanogaster to show that activation of a factor called SIK3 by insulin dampens a well-characterized pathway promoting fat breakdown, providing a molecular link between and lipid storage.

"The is like a hybrid car. In the daytime we use glucose as high octane fuel, but at night we switch to the battery, which in this case is stored fat," says Montminy. "This new study shows how SIK3 promotes storage during daytime feeding hours by blocking fat breakdown programs that normally only function during night-time fasting periods."

During fasting, a group of fat-busting enzymes, called lipases, trigger the flow of energy from the fly's low-power battery fat pack to different organs in the body. These lipases are turned on by a , called FOXO, part of the central transmission for fasting metabolism. When the flies eat food, SIK3 shuts off the FOXO switch, which both cuts off the battery's energy stream by silencing the fat-busting enzymes and allowing the "fat pack" to recharge its batteries.

Having teamed up previously to analyze pathways regulating glucose availability, Montminy, an expert in metabolism, and Thomas, a fly geneticist, focused on SIK3 in part because it is expressed in the fly fat body-a structure equivalent to mammalian adipose tissue and liver-but primarily because it is the Drosophila counterpart of a mammalian liver enzyme that antagonizes fat breakdown.

In experiments led by Biao Wang, Ph.D., a postdoctoral fellow in the Montminy lab and the paper's first author, the team mutated the Drosophila SIK3 gene, thereby disabling it, and monitored changes in . Mutant flies showed abnormally meager fat stores in the fat body and rapidly starved to death when deprived of food. "A normal fly can store enough fat to survive that period of food deprivation," says Thomas. "Flies in the wild lacking SIK3 would not make it from one feeding to the next."

That lack of fat was explained in part by the team's observation that SIK3 indirectly represses expression of a fat-burning enzyme active only in times of starvation. But the group immediately suspected that SIK3 was antagonizing a much bigger metabolic fish, namely a well-characterized "master regulator" known as FOXO, which in many organisms works in the nucleus to switch on genes that promote fat burning in times of nutrient deprivation.

Unexpectedly, SIK3 does not control the FOXO switch directly. Rather, much like a runner in a relay race, the SIK3 enzyme had to pass the baton to another enzyme called HDAC4, which in turn regulates FOXO.

"The complexity of this molecular machine likely reflects its importance in determining when the batteries should be turned on or off," says Montminy. "Indeed, perhaps the best argument for the importance of a group of molecules is that you see them doing the same thing over and over again in different organisms."

The investigators found that the SIK3/HDAC4/FOXO machine they had characterized in the fruitfly also controls the metabolic hybrid engine in mice. There, disabling one of these molecules in liver also disrupted the metabolic switch from fasting to feeding.

Ease of genetic manipulation makes flies a popular model organism for biological research. But ease was not the primary motivator of these studies. "Virtually all important components of the insulin pathway are conserved in flies and mammals," says Montminy. "Numerous human disease genes are expressed in Drosophila, and you can even mimic certain aspects of diabetes in fly models as well."

Thomas agrees, suggesting that metabolic similarities between flies and mammals exemplify mother nature's reluctance to improve on a good thing, especially when that good thing determines life or death. "The fact that these same pathways are used wholesale in flies and humans is quite striking," says Thomas. "A fly has to regulate its metabolism just like a human-if you create something during evolution that works well, it's likely going to remain conserved."

Unraveling SIK3/HDAC4/FOXO regulatory activity puts, as Thomas says, "more pharmacological possibilities on the table" in treating metabolic disease, an opinion echoed by Montminy.

"Currently, we have over 20 million people with type 2 diabetes and close to 60 million with insulin resistance," says Montminy. "This is a huge problem tied to obesity. Finding a way to curb obesity will essentially require consideration of both environmental and genetic factors. The human counterparts of HDAC4 and SIK3 may be mutated in ways that make them work less effectively and enhance our proclivity to become obese."

Explore further: 'Fasting pathway' points the way to new class of diabetes drugs

Related Stories

'Fasting pathway' points the way to new class of diabetes drugs

May 12, 2011
A uniquely collaborative study by researchers at the Salk Institute for Biological Studies uncovered a novel mechanism that turns up glucose production in the liver when blood sugar levels drop, pointing towards a new class ...

Recommended for you

Want to win at sports? Take a cue from these mighty mice

July 20, 2017
As student athletes hit training fields this summer to gain the competitive edge, a new study shows how the experiences of a tiny mouse can put them on the path to winning.

'Smart' robot technology could give stroke rehab a boost

July 19, 2017
Scientists say they have developed a "smart" robotic harness that might make it easier for people to learn to walk again after a stroke or spinal cord injury.

Engineered liver tissue expands after transplant

July 19, 2017
Many diseases, including cirrhosis and hepatitis, can lead to liver failure. More than 17,000 Americans suffering from these diseases are now waiting for liver transplants, but significantly fewer livers are available.

Lunatic Fringe gene plays key role in the renewable brain

July 19, 2017
The discovery that the brain can generate new cells - about 700 new neurons each day - has triggered investigations to uncover how this process is regulated. Researchers at Baylor College of Medicine and Jan and Dan Duncan ...

New animal models for hepatitis C could pave the way for a vaccine

July 19, 2017
They say that an ounce of prevention is worth a pound of cure. In the case of hepatitis C—a disease that affects nearly 71 million people worldwide, causing cirrhosis and liver cancer if left untreated—it might be worth ...

Omega-3 fatty acids fight inflammation via cannabinoids

July 18, 2017
Chemical compounds called cannabinoids are found in marijuana and also are produced naturally in the body from omega-3 fatty acids. A well-known cannabinoid in marijuana, tetrahydrocannabinol, is responsible for some of its ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.