Egg's movements predict embryo's chance of survival, study suggests
Researchers at the University of Cambridge have developed a new technique which could significantly increase success rates of pregnancies and reduce the frequency of multiple pregnancies associated with in vitro fertilisation (IVF).
Normally, embryos are implanted after two or three days in culture, with their progress at this stage used as a predictor of their likely development. However, around half of all human embryos stop developing before the blastocyst stage at day five of development. As a result, patients are often implanted with many embryos at once, which can lead to multiple pregnancies.
In order to improve success rates and reduce the number of multiple pregnancies, which are potentially risky for the mother and often end in miscarriage, many IVF clinics delay implantation until embryos have reached the blastocyst stage, and implant fewer embryos per round of treatment.
“There is a need to ensure that the embryos which are implanted into the mothers are those which stand the greatest chance of resulting in a successful live birth,” said lead author Professor Magdalena Zernicka-Goetz of The Wellcome Trust/Cancer Research UK Gurdon Institute at the University of Cambridge.
“It is important to be able to quantitate some indication of the health of embryos at the earliest possible stage in order to minimise the time they have to spend outside the body of the mother.”
Professor Zernicka-Goetz with her team and her co-authors from the University of Oxford and Cardiff University have developed a non-invasive method of accurately predicting the likelihood that an embryo will develop not only to the blastocyst stage, but also to birth from as early as two hours after fertilisation.
The researchers discovered that fertilisation initiates a series of pulsating movements in the cytoplasm, the jelly-like substance which fills the cell. The movements are caused by an interaction between the sperm and a part of the egg known as the cortex. They coincide with pulsations of the fertilisation cone, which is formed when the sperm and egg plasma membranes fuse to draw the sperm into the egg.
By constructing time-lapse recordings of the cytoplasmic movements and pulsations of the fertilisation cone over a period of two hours after fertilisation, the researchers charted the frequency of these movements in a study involving fertilised mouse eggs. These recordings showed a linear relationship between the speed of the movements and the likelihood that an embryo will develop to the blastocyst stage. This allowed the group to develop a mathematical model enabling them to predict successful development beyond this stage – all the way to birth.
Since similar movements occur in human embryos, these movements should be similarly predictive of embryo viability in humans. The invention provides a quantitative method of determining embryo viability which is much more reliable than current qualitative visual inspection. The work is being supported in their commercialisation efforts by Cambridge Enterprise, the University’s commercialisation group.
“In vitro fertilisation is an emotionally and physically demanding experience for prospective parents, with current success rates of only 30 per cent,” said Dr Steven Suchting of Cambridge Enterprise. “Improving success rates for IVF by choosing the best embryo to implant not only decreases the risks associated with multiple embryo transfers but also potentially minimises the number of rounds of IVF that potential mothers must go through in order to achieve a successful pregnancy. This saves prospective parents both the trauma and cost of the IVF experiences.”
