New way to harvest stem cells better for donors

March 15, 2016, CSIRO
A hematopoietic stem cell (HSC) is being mobilized from the bone marrow microenvironment into a blood vessel. Credit: Dr Kate Patterson / Garvan Institute ofMedical Research, NSW

Australian scientists have developed a new method for harvesting stem cells, which is less invasive and reduces side effects for donors.

For bone marrow transplantation, are routinely harvested from healthy donors and used to treat patients with cancers including leukaemia.

Current harvesting methods take a long time and require injections of a growth factor to boost stem cell numbers. This often leads to side effects.

The discovery, published today in Nature Communications, reduces the time required to obtain adequate numbers of stem cells, without the need for a growth factor.

The method, developed by a team of CSIRO researchers working within the manufacturing arm of CSIRO with the Australian Regenerative Medicine Institute (ARMI) at Monash, combines a newly discovered molecule (known as BOP), with an existing type of molecule (AMD3100) to mobilise the stem cells found in bone marrow out into the blood stream.

CSIRO researcher Dr Susie Nilsson said her team was able to demonstrate that combining the two molecules directly impacts stem cells so they can be seen in the within an hour of a single dosage.

"Current treatment requires the patient to have growth factor injections for several days leading up to the procedure," Dr Nilsson said.

"Using the new method eliminates the need for this, meaning a procedure that once took days can be reduced to around an hour."

Until now AMD3100 has only been effective in increasing stem cell numbers when combined with the growth factor.

"But the growth factor can cause unpleasant side effects like bone pain and spleen enlargement for some patients," Dr Nilsson said.

"Other patients simply don't respond well, and their stem cell count never gets high enough for a successful transplant."

The scientists found that combining the two small molecules not only eliminates the need for the growth factor, but when the harvested cells are transplanted they can replenish the entire system, and there are no known side effects.

Professor Peter Currie, ARMI Director, said a major benefit of the discovery is that harvesting stem cells will become more efficient and effective, considerably reducing the stress for donors.

"We're looking forward to seeing patients benefit from this discovery," Professor Currie said.

So far successful pre-clinical studies have demonstrated the effectiveness of the treatment.

The next step is a phase 1 clinical trial assessing the combination of BOP molecule with the , prior to the eventual successful combination of the two small molecules BOP and AMD3100.

Explore further: Blood stem cells study could pave the way for new cancer therapy

More information: Benjamin Cao et al. Therapeutic targeting and rapid mobilization of endosteal HSC using a small molecule integrin antagonist, Nature Communications (2016). DOI: 10.1038/ncomms11007

Related Stories

Blood stem cells study could pave the way for new cancer therapy

March 10, 2016
People with leukaemia could be helped by new research that sheds light on how the body produces its blood supply.

Scientists find way to predict activity of stem cells

February 29, 2016
Scientists from the Florida campus of The Scripps Research Institute (TSRI) have for the first time developed a way to predict how a specific type of stem cell will act against different diseases. With more than 500 stem ...

Newly identified genes impact how transplanted stem cells give rise to blood cells

February 16, 2016
A team of researchers led by scientists at St. Jude Children's Research Hospital is looking at ways to improve how blood-forming stem cells can be used for therapeutic interventions. The work has uncovered a group of genes ...

Team identifies emergency response system for blood formation

November 16, 2015
Scientists at the Children's Medical Center Research Institute at UT Southwestern (CRI) have determined how the body responds during times of emergency when it needs more blood cells. In a study published in Nature, researchers ...

Researchers find NSAIDs help push stem cells into bloodstream prior to transplantation

March 14, 2013
(Medical Xpress)—A team of researchers at Indiana University's School of Medicine has found that giving meloxicam, a non-steroidal anti-inflammatory drug (NSAID), to people and baboons boosts the number of haematopoietic ...

Source of stem cells used for bone marrow failure treatment varies worldwide

January 12, 2016
Ayami Yoshimi, M.D., Ph.D., of the University of Freiburg, Germany, and colleagues examined the use of peripheral blood stem cells and bone marrow as stem cell sources for hematopoietic stem cell transplantation in patients ...

Recommended for you

Japanese team creates human oogonia using human stem cells in artificial mouse ovaries

September 21, 2018
A team of researchers with members from several institutions in Japan has successfully generated human oogonia inside of artificial mouse ovaries using human stem cells. In their paper published in the journal Science, the ...

A new approach to developing a vaccine against vivax malaria

September 21, 2018
A novel study reports an innovative approach for developing a vaccine against Plasmodium vivax, the most prevalent human malaria parasite outside sub-Saharan Africa. The study led by Hernando A. del Portillo and Carmen Fernandez-Becerra, ...

Researchers explore how changes in diet alter microbiome in artificial intestine

September 21, 2018
Using an artificial intestine they created, researchers have shown that the microbiome can quickly adapt from the bacterial equivalent of a typical western diet to one composed exclusively of dietary fats. That adaptation ...

Study identifies stem cell that gives rise to new bone and cartilage in humans

September 20, 2018
A decade-long effort led by Stanford University School of Medicine scientists has been rewarded with the identification of the human skeletal stem cell.

Scientists grow human esophagus in lab

September 20, 2018
Scientists working to bioengineer the entire human gastrointestinal system in a laboratory now report using pluripotent stem cells to grow human esophageal organoids.

Researchers identify human skeletal stem cells

September 20, 2018
Human skeletal stem cells that become bone, cartilage, or stroma cells have been isolated from fetal and adult bones. This is the first time that skeletal stem cells, which had been observed in rodent models, have been identified ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.