Novel reagent detects memory immune response in vaccinated animals

Novel reagent detects memory immune response in vaccinated animals
Credit: Mary Ann Liebert, Inc., publishers

Researchers have developed a novel reagent capable of detecting rare, antigen-specific B cells that indicate successful vaccination in veterinary animals. The method used to create a B cell tetramer that can detect a memory immune response to porcine reproductive and respiratory syndrome virus (PRRSV) will be widely applicable for demonstrating immunity to other veterinary pathogens, and is presented in an article in Viral Immunology.

The article entitled "B Cell Tetramer Development for Veterinary Vaccinology" is part of a special issue on Challenges in Veterinary Vaccines led by Guest Editor Crystal Loving, PhD, USDA-ARS National Animal Disease Center, Ames, IA.

Coauthors Michael Rahe, Kevin Gustafson, and Michael Murtaugh, University of Minnesota, St. Paul, described how the nsp7-B cell tetramer can be used to facilitate an in-depth understanding of the characteristics and quality of the memory B cell response generated to PRRSV infection and to vaccination against the virus. The researchers reported that validation studies showed the reagent able to detect PRRSV-specific B present at a frequency of about 0.001% of the total B lymphocytes in a vaccinated animal.

"The authors have done a wonderful job of extending B cell tetramer technology to the veterinary immunology field. This approach will be tremendously useful for the study of veterinary vaccines in general," says David L. Woodland, PhD, Editor-in Chief of Viral Immunology and Chief Scientific officer for Keystone Symposia on Molecular and Cellular Biology.

Research reported in this publication was supported by grants from National Institutes of Health under Award Number T32 OD010993. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.


Explore further

Researchers identify immune component up-regulated in brain after viral infection

More information: Michael C. Rahe et al, B Cell Tetramer Development for Veterinary Vaccinology, Viral Immunology (2017). DOI: 10.1089/vim.2017.0073
Journal information: Molecular and Cellular Biology

Citation: Novel reagent detects memory immune response in vaccinated animals (2017, October 16) retrieved 20 May 2019 from https://medicalxpress.com/news/2017-10-reagent-memory-immune-response-vaccinated.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
1 shares

Feedback to editors

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more