Convergence of synaptic signals is mediated by a protein critical for learning and memory

Convergence of synaptic signals is mediated by a protein critical for learning and memory
(TOP) Average time course of PKCα activity measured by ITRACKα biosensor during structural Long-Term Potentiation (sLTP).(BOTTOM) PKC activity was compartmentalized in the stimulated spine, suggesting a local, spine-specific role in plasticity. White spot indicates location of glutamate uncaging. Credit: Max Planck Florida Institute for Neuroscience

Inside the brain, is a complex symphony of perfectly coordinated signaling. Hundreds of different molecules amplify, modify and carry information from tiny synaptic compartments all the way through the entire length of a neuron. The precise interplay of these proteins is critical for normal neuronal function; ultimately allowing the brain to achieve feats like cognition, decision making, and sensory perception.

Researchers from the lab of Ryohei Yasuda at the Max Planck Florida Institute for Neuroscience (MPFI), study the elaborate nexus of signaling proteins involved in learning and memory; looking at a unique process called synaptic plasticity, the innate ability of a neural synapse to either strengthen or weaken. Understanding how proteins behave during plasticity is key to unlocking how we learn and how memories are formed.

In a study published in Nature Neuroscience in July 2018, Dr. Lesley Colgan, a Research Fellow in the Yasuda Lab and leading author of the paper, delves into the role that Protein Kinase C (PKC) plays in synaptic plasticity. PKC represents an entire family of 12 distinct kinases, that propagate a biochemical signal through the phosphorylation of target proteins they interact with. While previously implicated in synaptic plasticity, engineering tools sensitive enough to study PKC in the brain has been a major challenge. Yasuda's team developed a host of sensors that can track the activity of PKCs with unprecedented specificity and sensitivity, probing when and where activation occurs as well as how these enzymes turn on and off. With these powerful sensors, they have been able to look more closely at the role that PKC plays at the single synapse level.

This study demonstrates that PKCα is a highly versatile molecule, capable of assimilating many disparate sources of information. Because of this adaptability, the protein is well poised as a mediator of the processes underlying synaptic plasticity and learning. Credit: Max Planck Florida Institute for Neuroscience

One enzyme in the PKC family, PKC alpha, plays a direct role in facilitating synaptic plasticity. When PKC alpha activity is perturbed, plasticity is subsequently disrupted; demonstrating that the protein is a critical first step in the initial molecular cascade. PKC alpha also has very robust activation and is rather spine specific, confining its activity to only the stimulated spine and not spreading to the surrounding dendrites.

A unique discovery of the study, found that PKC alpha acts to integrate two temporally distinctive signaling pathways both known to be critical for synaptic plasticity. The first is BDNF mediated, a slower, diffuse signal developing over minutes and the second, is NMDA receptor mediated calcium influx, a spine-specific signal occurring in a matter of milliseconds and necessary to begin the rapid molecular cascade of . Adaptably responding to both of these signals, PKC alpha is able to sample recent synaptic history and integrate it with current synaptic events, enabling complex processing of information at the single spine level.

Investigating these intriguing findings further, the team was able to successfully link molecules to memory, uncovering that PKC alpha is required for efficiency of learning. Mice with PKC alpha removed from the brain, take a significantly longer amount of time to become proficient at a learning task than counterparts containing intact PKC alpha . Despite this lengthier learning curve, if given additional training, mice lacking PKC alpha will eventually learn the task just as well as wild type mice; supporting the notion that PKC alpha is critical for encoding a memory but may not play a significant role in the recall of a memory once it's established.

Convergence of synaptic signals is mediated by a protein critical for learning and memory
A subthreshold stimulus that was unable to induce plasticity was given alone (unpaired) or after a nearby plasticity-inducing stimulation (paired). Unpaired, the stimulus could not activate PKCα or induce plasticity. However, when paired with recent plasticity in a nearby spine, which induces a long-lasting, spreading TrkB activity, this same subthreshold stimulus led to PKCα activation and plasticity. Credit: Max Planck Florida Institute for Neuroscience

This work represents a substantial advancement in the field, providing integral tools that will enable the disentanglement of complex signaling related to learning and . According to Dr. Colgan, "These newly developed sensors will have broad usage in neuroscience and cell biology alike, allowing the study of many complex signaling pathways that PKC has a hand in."


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More information: Lesley A Colgan et al, PKCĪ± integrates spatiotemporally distinct Ca2+ and autocrine BDNF signaling to facilitate synaptic plasticity, Nature Neuroscience (2018). DOI: 10.1038/s41593-018-0184-3
Journal information: Nature Neuroscience

Citation: Convergence of synaptic signals is mediated by a protein critical for learning and memory (2018, July 16) retrieved 19 October 2019 from https://medicalxpress.com/news/2018-07-convergence-synaptic-protein-critical-memory.html
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