Bioengineering approach for functional muscle regeneration

Bioengineering approach for functional muscle regeneration
The dECM-MA derived from porcine skeletal muscles is combined with PVA fibrillation, which provides the topographical cues for proper cellular alignment when bioengineering rat muscles. Credit: Sang Jin Lee

When trauma, illness, or injury causes significant muscle loss, reconstructive procedures for bioengineering functional skeletal muscles can fall short, resulting in permanent impairments.

Finding a synergy in the importance of biochemical signals and topographical cues, researchers from Wake Forest Institute for Regenerative Medicine, Sungkyunkwan University, and Chonnam National University developed an efficient technique for and functional restoration in injured rats. They describe results from the technique in the journal Applied Physics Reviews, from AIP Publishing.

The group expanded on a method they previously developed using muscle-specific materials derived from an organism's tissues (dECM-MA) to construct bioinks, which are materials used for 3D-printing .

They combined dECM-MA from pigs' skeletal muscles with pol(yvinyl alcohol) (PVA) fibrillation, a technique that provides cues to the molecules in the bioink to guide them to their target tissue and ensure they are properly aligned. By optimizing the PVA to enable stable, viable, well-aligned cell structures, the researchers were able to improve muscle regeneration and function restoration.

"One major benefit over previous approaches is the self-alignment of muscle cells in the 3D structure without any supporting components," said Sang Jin Lee, one of the authors. "This could allow us to fabricate more clinically relevant bioengineered muscle constructs."

They tested the technique on rats with injured muscles in their feet. When compared with uninjured rats of the same age and rats with the same injury but no treatment, the rats treated with the muscle regeneration technique showed over 80% muscle function restoration. Moreover, the bioengineered muscles integrated well with the ' neural and vascular systems.

These promising results suggest combining dECM-MA with PVA is a clinically feasible method for achieving large-scale tissue regeneration, provided the damage does not extend to nearby cells from which the tissue materials can be derived.

"This advanced bioprinting approach for bioengineering functional skeletal muscle constructs may be an effective therapeutic option for treating extensive muscle defect injuries with accelerated innervation and vascularization," Lee said.

Since the technique requires cells from the patient, the group anticipates some hurdles in translating the methodology for , where it will be especially beneficial for systems that require cellular-level alignment, like cardiac and skeletal muscles. In the meantime, they plan to continue preclinical trials on larger, more clinically relevant constructs in larger animals, such as rabbits, dogs, and pigs.

More information: Hyeongjin Lee et al. Self-aligned myofibers in 3D bioprinted extracellular matrix-based construct accelerate skeletal muscle function restoration
Applied Physics Reviews (2021); DOI: 10.1063/5.0039639

Journal information: Applied Physics Reviews
Citation: Bioengineering approach for functional muscle regeneration (2021, May 11) retrieved 26 February 2024 from
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